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Ecological toilet treatment strain and application thereof

A toilet and bacillus technology, applied in the field of environmental microorganisms, can solve the problems of reducing pathogenic microorganisms, less than ideal effect, restricting the application of microorganisms, etc., and achieve the effect of wide temperature range

Active Publication Date: 2020-02-14
SINO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Japan and Europe have achieved rich results in the study of microbial degradation of feces, while my country has self-developed functional microorganisms, but the effect is not satisfactory
In addition, the growth environment of microbial strains is relatively harsh. If the environment is not suitable, that is, the temperature and water activity cannot meet the appropriate conditions, the efficiency of microorganisms to decompose organic matter in feces and kill pathogenic microorganisms will be greatly reduced.
Therefore, the toilet, which is an environment with relatively harsh conditions for the growth of microorganisms, severely restricts the application of microorganisms in ecological toilets. The development of efficient and functional microbial strains is an urgent and difficult point to break through

Method used

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  • Ecological toilet treatment strain and application thereof
  • Ecological toilet treatment strain and application thereof
  • Ecological toilet treatment strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Screening, functional verification and strain identification of manure-degrading bacteria in ecological toilets

[0024] The samples used in the invention for screening the manure-degrading bacteria in ecological toilets are collected in a rural dry toilet. The samples were subjected to experiments such as enrichment culture, separation and purification, fecal degradation function verification, and strain identification, and finally the strain BM-Y3 was obtained. BM-Y3 was identified as Bacillus Mojaves ( Bacillus mojavensis ), preserved in China Center for Type Culture Collection (CCTCC), address: Wuhan University, Wuhan, China; strain classification and name: Bacillus mojave desert Bacillus mojavensis BM-Y3, deposit date: October 12, 2019, deposit number: CCTCC M 2019815.

[0025] Enrichment culture: Take 5 g of collected samples and inoculate them in a conical flask filled with 100 mL of liquid medium for culture. Liquid medium composition: peptone 10 g...

Embodiment 2

[0033]Example 2: Bacillus mojave desert Bacillus mojavensis Preparation of BM-Y3 bacterial agent

[0034] Preparation of liquid bacterial agent: Inoculate BM-Y3 pure bacteria into 10 mL liquid seed medium, culture at 30 °C and 200 r / min for 24 h, and then insert the same inoculum with a volume fraction of 1%. Expand the culture in the liquid medium, and cultivate it for 24 hours under the conditions of 30 °C and 200 r / min to obtain the liquid bacterial agent of the strain.

[0035] Preparation of solid inoculum: It is prepared by mixing liquid inoculum of BM-Y3 strain with wood chips and drying, wherein the mass ratio of wood chips to liquid inoculum is 1:2.

Embodiment 3

[0036] Example 3: Bacillus mojave desert Bacillus mojavensis Treatment effect of BM-Y3 on human feces

[0037] Add BM-Y3 strain solid bacterial agent to the human feces to be treated. The mass ratio of solid bacterial agent to feces is 1:3. After stirring evenly, carry out aerobic fermentation at 40 ° C. During the process, uninterrupted Ventilate and stir to ensure uniform heating and under aerobic conditions. The treatment system was weighed every 12 hours to obtain the solid degradation rate of manure, and the weight reduction effect was as follows: figure 2 shown. From figure 2 It can be seen from the figure that the experimental group inoculated with the strain BM-Y3 has always presented a higher wet weight loss rate than the uninoculated control group, and the wet weight loss rate of the inoculated strain BM-Y3 reached 76 at 48 h. %, 46% higher than 52% in the control group.

[0038] At the same time, samples were taken every 12 hours to measure the content of dry...

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Abstract

The invention relates to the technical field of environmental microorganisms, in particular to an ecological toilet treatment strain and an application thereof. The invention provides bacillus mojavensis BM-Y3. A preservation number of the bacillus mojavensis BM-Y3 is CCTCC NO: M 2019815. The decomposition rate of organic matters in the excrement can be effectively increased by the bacillus mojavensis BM-Y3; the strain can be used for reduction and stabilization treatment of excrement in the ecological toilet treatment process, and has high application value.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to an ecological toilet treatment strain and its application. Background technique [0002] Toilets play a vital role in people's daily life, receiving, accumulating and eliminating most of the waste excreted in human's daily physiological activities every day. After these manures are discharged into urban sewers, they become an important source of pollutants in domestic sewage and are also an important part of urban sewage pollutants. Therefore, the problem of environmental pollution caused by manure has been paid more and more attention by management departments. [0003] In today's society that advocates "environmental protection", "green" and "ecology", ecological toilets have emerged as the times require. Ecological toilet refers to a type of toilet that does not pollute the environment, can make full use of various resources, and emphasizes t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F11/02C12R1/07
CPCC02F11/02C12N1/20C12N1/205C12R2001/07
Inventor 姜传胜时公玉付林周志刚王光磊肖潇段夕强慕进忠杨斐然
Owner SINO TECH CO LTD
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