Marine bacteria capable of antagonizing two important pathogenic vibrios of marine culture and application of marine bacteria

Abstract

The invention relates to marine bacteria capable of antagonizing two important pathogenic vibrios of marine culture and an application of the marine bacteria. The antagonistic bacteria are marine-derived pseudoalteromonas agarivorans and are preserved in the common microorganism center of the China General Microbiological Culture Collection Center, and the preservation number of the antagonistic bacteria is CGMCC No17154. The pseudoalteromonas screened by the method is separated from Antarctic ice sea water, and the marine bacteria capable of antagonizing important pathogenic vibrio anguillarum and vibrio alginolyticus in aquaculture is a biological prevention and control potential strain with high prevention and control efficiency, wide prevention and control range and good environmentalsafety, and has good development and application prospects.

Description

technical field

[0001] The invention belongs to the technical field of microorganism prevention and control, and in particular relates to a pseudoalteromonas capable of antagonizing two kinds of seawater cultured pathogenic Vibrio and application thereof. Background technique

[0002] In recent years, my country's mariculture industry has developed rapidly and has become a major aquaculture country in the world. However, with the continuous expansion of aquaculture scale and the continuous improvement of intensification, the pollution of aquaculture water is becoming more and more serious, and many diseases have appeared one after another, especially bacterial diseases. Cause serious losses to the aquaculture industry. The bacterial pathogens reported at home and abroad mainly include Vibrio anguillarum, Vibrio solendidus, Vibrio parahaemolyticus, Vibrio pelagius, and Vibrio alginolyticus. bacteria (Vibrio alginolyticus) and mermaid Vibrio (Vibrio damsela), etc. In addition...

Examples

Embodiment 1

[0013] Example 1 Isolation, Identification and Preservation of Pseudoalteromonas

[0014] The screening method of the above-mentioned antagonistic bacteria is as follows: firstly, the pathogenic bacteria Vibrio anguillarum (Vibrio anguillarum) and alginolyticus (vibrio parahemolyticus) were used as indicator bacteria respectively, and more than 300 strains of bacteria isolated from Antarctic ice seawater and turbot breeding pond water were used, The antagonistic bacteria were screened by spot method and filter paper method. The extracellular products of antagonistic bacteria obtained through screening will be used for the prevention of hemorrhage and ulcer epidemic diseases in turbot fins. The specific technical scheme is as follows:

[0015] Screening of antagonistic bacteria: Inoculate the indicator bacteria Vibrio anguillarum and vibrioparahemolyticus in LB liquid medium containing 3% NaCl respectively, culture with shaking at 28°C for 12-16 hours, take 40μl-50μl Spread t...

Embodiment 2

[0024] Example 2 Application of antagonistic bacteria Pseudoalteromonas agarivorans

[0025] 100 healthy and uniform turbots (average body length 10±1cm, average body weight 12±2g) were raised in a 30L seawater storage tank. During the experiment, the water was continuously aerated at a temperature of 20°C and the water was changed every day. Do not cast bait. Vibrio anguillarum, Vibrio parahemolyticus and Pseudoalteromonas agarivorans isolated by screening were inoculated in LB medium containing 3% NaCl, and cultured at 28°C for 24 hours. Vibrio anguillarum and Vibrio alginolyticus were centrifuged and resuspended in sterile sea water respectively to obtain the bacterial suspension. Pseudoalteromonas was centrifuged to discard the precipitate, and the supernatant was filtered through a 0.22 μm microporous membrane. Carry out the following three groups of experimental treatments, 30 tails in each group; (1) the control group, without adding pathogenic bacteria and antagonisti...

technical field

[0001] The invention belongs to the technical field of microorganism prevention and control, and in particular relates to a pseudoalteromonas capable of antagonizing two kinds of seawater cultured pathogenic Vibrio and application thereof. Background technique

[0002] In recent years, my country's mariculture industry has developed rapidly and has become a major aquaculture country in the world. However, with the continuous expansion of aquaculture scale and the continuous improvement of intensification, the pollution of aquaculture water is becoming more and more serious, and many diseases have appeared one after another, especially bacterial diseases. Cause serious losses to the aquaculture industry. The bacterial pathogens reported at home and abroad mainly include Vibrio anguillarum, Vibrio solendidus, Vibrio parahaemolyticus, Vibrio pelagius, and Vibrio alginolyticus. bacteria (Vibrio alginolyticus) and mermaid Vibrio (Vibrio damsela), etc. In addition...

Examples

Embodiment 1

[0013] Example 1 Isolation, Identification and Preservation of Pseudoalteromonas

[0014] The screening method of the above-mentioned antagonistic bacteria is as follows: firstly, the pathogenic bacteria Vibrio anguillarum (Vibrio anguillarum) and alginolyticus (vibrio parahemolyticus) were used as indicator bacteria respectively, and more than 300 strains of bacteria isolated from Antarctic ice seawater and turbot breeding pond water were used, The antagonistic bacteria were screened by spot method and filter paper method. The extracellular products of antagonistic bacteria obtained through screening will be used for the prevention of hemorrhage and ulcer epidemic diseases in turbot fins. The specific technical scheme is as follows:

[0015] Screening of antagonistic bacteria: Inoculate the indicator bacteria Vibrio anguillarum and vibrioparahemolyticus in LB liquid medium containing 3% NaCl respectively, culture with shaking at 28°C for 12-16 hours, take 40μl-50μl Spread t...

Embodiment 2

[0024] Example 2 Application of antagonistic bacteria Pseudoalteromonas agarivorans

[0025] 100 healthy and uniform turbots (average body length 10±1cm, average body weight 12±2g) were raised in a 30L seawater storage tank. During the experiment, the water was continuously aerated at a temperature of 20°C and the water was changed every day. Do not cast bait. Vibrio anguillarum, Vibrio parahemolyticus and Pseudoalteromonas agarivorans isolated by screening were inoculated in LB medium containing 3% NaCl, and cultured at 28°C for 24 hours. Vibrio anguillarum and Vibrio alginolyticus were centrifuged and resuspended in sterile sea water respectively to obtain the bacterial suspension. Pseudoalteromonas was centrifuged to discard the precipitate, and the supernatant was filtered through a 0.22 μm microporous membrane. Carry out the following three groups of experimental treatments, 30 tails in each group; (1) the control group, without adding pathogenic bacteria and antagonisti...