Method of measuring N2O emission rate and source contribution rate of alpine grassland

Abstract

The invention discloses a method of measuring the N2O emission rate and the source contribution rate of alpine grassland. The method comprises the following steps of: 1, collecting a soil sample in the alpine grassland, 2, dividing the soil sample into three treatment groups, 3, collecting gas released by the soil, and measuring the concentration of N2O and isotope characteristic values delta<15>N<alpha>, delta<15> N<bulk> and delta18O of the N2O, 4, calculating the emission rate of N2O of each treatment group and the isotope site preference SP of the N2O, 5, calculating to obtain delta<18>ONiD of N2O emitted by denitrification of the nitrifying bacteria, and 6, calculating respective contribution rates of the nitrification effect, the denitrification effect and the denitrification effectof the nitrifying bacteria. By adopting the method, the contribution of nitrification and denitrification of nitrifying bacteria to N2O emission can be accurately and quantitatively identified, and atheoretical basis and a technical support are provided for researching an emission path and a mechanism of N2O in the alpine grassland.

Description

technical field [0001] The present invention relates to the greenhouse gas N 2 The source research field of O, specifically a kind of alpine grassland N 2 O emission rate and source contribution rate calculation method. Background technique [0002] Grassland is a greenhouse gas N 2 An important emission source of O. Soil microorganisms produce N through nitrification, denitrification, and denitrification of nitrifying bacteria. 2 The process of O is regulated by various factors such as soil texture, temperature, and moisture. Due to the limitations of research methods and means, the N produced by different processes 2 The precise quantification of the contribution of O to emissions is still insufficient, which limits the understanding of the nitrogen cycle and its response to warming. Therefore, grassland N 2 There are still great uncertainties in the dynamic law of O emission, the identification and quantification of different production processes, and its response t...

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Problems solved by technology

[0007] In order to overcome the deficiencies in the prior art, the invention provides a kind of alpine grassland N 2 The calculation method of O emission rate and source contribution rate solves the lack of understanding of alpine grassland N in the existing technology 2 The research on O emission and its mechanism is unable to study the effect of nitrification, denitrification and denitrification of nitrifying bacteria on N. 2 The problem of making precise quantification of O emission contribution

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