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a kind of alpine meadow n 2 oMeasurement method of emission rate and source contribution rate

A technology of emission rate and contribution rate, applied in the field of calculation of N2O emission rate and source contribution rate of alpine grassland, can solve the problems of lack of emission and mechanism

Active Publication Date: 2022-06-21
成都栢晖生物科技有限公司
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Problems solved by technology

[0007] In order to overcome the deficiencies in the prior art, the invention provides a kind of alpine grassland N 2 The calculation method of O emission rate and source contribution rate solves the lack of understanding of alpine grassland N in the existing technology 2 The research on O emission and its mechanism is unable to study the effect of nitrification, denitrification and denitrification of nitrifying bacteria on N. 2 The problem of making precise quantification of O emission contribution

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  • a kind of alpine meadow n <sub>2</sub> oMeasurement method of emission rate and source contribution rate
  • a kind of alpine meadow n <sub>2</sub> oMeasurement method of emission rate and source contribution rate
  • a kind of alpine meadow n <sub>2</sub> oMeasurement method of emission rate and source contribution rate

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Embodiment

[0042] A kind of alpine grassland N shown in the preferred embodiment of the present invention 2 The calculation method of O emission rate and source contribution rate includes the following steps:

[0043] Step 1: Collect soil samples in the alpine grassland, preferably soil with a thickness of 0-20cm. The so-called "soil with a thickness of 0-20cm" refers to 0-20cm from the ground, and the soil within this depth range is also called It is surface soil or shallow soil. It is beneficial to obtain more realistic experimental results by taking the soil within this depth range. After taking the soil, pass it through a 2.0mm sieve, then mix it evenly, remove the plant roots and stones in the soil, and then use an incubator Bring the soil samples back to the laboratory for dark cultivation. The cultivation temperature is preferably 10-15 °C. It is used for gas suppression cultivation experiments. During the cultivation process, the soil moisture is not adjusted, and the original so...

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Abstract

The invention discloses an alpine grassland N 2 The calculation method of O emission rate and source contribution rate includes the following steps: Step 1: Collect soil samples in the alpine grassland; Step 2: Divide the soil samples into three treatment groups; Step 3: Collect the gases released by the soil and measure N 2 concentration of O, and N 2 O isotopic eigenvalue δ 15 N α ,δ 15 N bulk and δ 18 O; Step 4: Calculate N for each treatment group 2 O emission rate and N 2 The isotope value SP of O; Step 5: Calculate the denitrification of nitrifying bacteria to discharge N 2 δ of O 18 o NiD ; Step 6: Calculate the respective contribution rates of nitrification, denitrification and denitrification of nitrifying bacteria. The present invention adopts method, can realize the denitrification of nitrification and nitrifying bacteria to N 2 Accurate quantitative identification of O emission contribution for alpine grassland N 2 The research on the emission pathway and mechanism of O provides theoretical basis and technical support.

Description

technical field [0001] The present invention relates to the greenhouse gas N 2 The field of research on the source of O, specifically an alpine grassland N 2 Calculation method of O emission rate and source contribution rate. Background technique [0002] Grass is the greenhouse gas N 2 An important source of O emission, soil microorganisms produce N through nitrification, denitrification, and denitrification by nitrifying bacteria. 2 The process of O is regulated by various factors such as soil texture, temperature and moisture. Due to the limitation of research methods and means, the N produced by different processes 2 Precise quantification of the contribution of O to emissions is still lacking, limiting the understanding of the nitrogen cycle and its response to warming. Therefore, grass N 2 There are still great uncertainties in the dynamic law of O emission, the identification and contribution quantification of different production processes, and their response t...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 李娜常瑞英周星梅王根绪陈建中徐毅蒋小强
Owner 成都栢晖生物科技有限公司
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