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An efficient and rapid method for analyzing the characteristic volatile components of Eucommia ulmoides leaves and its fermentation products

A technology for volatile components and fermentation products, which is applied in the field of efficient and rapid analysis of the characteristic volatile components of Eucommia ulmoides leaves and its fermentation products, to achieve the effect of improving efficiency, easy operation, and high-throughput analysis

Active Publication Date: 2022-04-01
INST OF ANALYSIS GUANGDONG ACAD OF SCI (CHINA NAT ANALYTICAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there are few reports on the study of volatile components in Eucommia ulmoides leaves and its fermentation products by gas phase ion mobility spectrometry

Method used

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  • An efficient and rapid method for analyzing the characteristic volatile components of Eucommia ulmoides leaves and its fermentation products
  • An efficient and rapid method for analyzing the characteristic volatile components of Eucommia ulmoides leaves and its fermentation products
  • An efficient and rapid method for analyzing the characteristic volatile components of Eucommia ulmoides leaves and its fermentation products

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Experimental program
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Embodiment 1

[0027] (1) Prepare samples to be tested of Eucommia leaves and the fermentation products of Eucommia leaves. Eucommia ulmoidesleaves (EUl) was collected in July 2018. After microwave cleaning, drying, and crushing, Eucommia ulmoides leaves powder samples (denoted as EUl) were obtained, and Eucommia ulmoides leaves were sealed and stored at low temperature; different Eucommia ulmoides leaves fermentation products Sample preparation: firstly, the strains of Ganoderma lucidum (Ganoderma Lucidum, GL), Hericium Erinaceus (HE) or Grifola frondosa (GF) were activated at 24°C and then transferred to the inclined surface of test tube PDA to prepare dense mycelium. , white and strong inclined mother species. Select several blocks of Ganoderma lucidum, Hericium erinaceus or Grifola frondosa from the parent species of the inclined plane, respectively insert them into the sterilized and cooled liquid culture medium, shake and culture them at 24°C for 20 days, and obtain a single liquid str...

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Abstract

The invention discloses an efficient and rapid method for analyzing characteristic volatile components of eucommia leaves and their fermentation products. Through the analysis and detection of different samples of Eucommia leaves and their fermentation products by gas phase-ion mobility spectrometry, three-dimensional qualitative analysis is carried out according to the migration rate, retention time and relative ion peak intensity, the corresponding spectra are drawn, and fingerprints are established, which can visually compare Eucommia At the same time, according to the characteristic information of different samples, select the characteristic area in the spectrum, qualitatively identify the characteristic volatile components, and further use data preprocessing methods and chemometric methods to realize rapid classification of samples . This method does not require complex pretreatment of samples, is simple to operate, and the characteristic area is fully visualized. It has broad application prospects in accurately and efficiently identifying the characteristic volatile components in Eucommia leaves and their fermentation products. For the high value of Eucommia resources It is of great significance for comprehensive utilization and sustainable development.

Description

technical field [0001] The invention belongs to the field of rapid analysis and detection, and in particular relates to a method for efficiently and rapidly analyzing characteristic volatile components of eucommia leaves and their fermentation products. Background technique [0002] Eucommia ulmoides Oliv. is a traditional nourishing medicinal material in my country. Eucommia ulmoides leaves contain many natural active ingredients, including iridoids, lignans and flavonoids, and are effective in regulating blood pressure, lowering blood sugar, lowering blood fat, enhancing immunity, anti-oxidation, antibacterial and anti-aging, etc. has a good effect. Eucommia ulmoides is very rich in resources in my country. It has been included in the "Chinese Pharmacopoeia" since 2005. In April 2018, the National Health and Medical Commission planned to manage eucommia ulmoides as both food and Chinese medicinal materials. Therefore, eucommia leaves in food , pharmaceuticals and cosmetic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/72G01N30/86
CPCG01N30/88G01N30/72G01N30/8686G01N2030/8809
Inventor 王志宏彭密军杨秋玲张命龙
Owner INST OF ANALYSIS GUANGDONG ACAD OF SCI (CHINA NAT ANALYTICAL
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