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A kit for precise structure identification of unsaturated fatty acids in oil

A technology of unsaturated fatty acid and kit, which is applied in the field of unsaturated fatty acid determination kit, which can solve the problems of complex derivatization reactions and the inability to accurately determine the position of double bonds, and achieve high-sensitivity and accurate structural analysis, accurate characterization, and use convenient effect

Active Publication Date: 2021-08-31
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional mass spectrometric identification method for the double bond position of fatty acids is gas chromatography tandem mass spectrometry, which can distinguish each fatty acid, but this method requires complex derivatization reactions on the sample, and can only accurately identify one double bond
For unsaturated fatty acids with a large number of double bonds, co-elution often occurs in gas chromatography, making it impossible to accurately determine the position of the double bond

Method used

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  • A kit for precise structure identification of unsaturated fatty acids in oil
  • A kit for precise structure identification of unsaturated fatty acids in oil
  • A kit for precise structure identification of unsaturated fatty acids in oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A kit for precise mass spectrometry structure identification of unsaturated fatty acids in oils, including alkaline hydrolyzate, hydrolysis reaction quencher, extract, PB reaction solution, DEEA reactant, salt cleaning agent, complex solution, the specific components are as follows :

[0039] ① Alkaline hydrolyzate: 0.35mol / L potassium hydroxide solution, 200μL;

[0040] ② Hydrolysis reaction quencher: 0.5mol / L hydrochloric acid, 500μL;

[0041] ③ Extraction solution: n-hexane, 9mL;

[0042] ④Patenò-Büchi (P-B) reaction solution: acetone / water / ethanol (570 / 380 / 50, v / v / v), 1 mL;

[0043] ⑤ DEEA reagent: N, N-diethylethylenediamine (N, N-diethyl-1, 2-ethanediamine, DEEA) solution: 20 μmol / mL, 50 μ L; 2-chloro-1-methylpyridinium iodide ( 2-chloro-1-methylpyridinium iodide (CMPI) solution: 20 μmol / mL, 50 μL; triethylamine (TEA) solution: 20 μmol / mL, 30 μL;

[0044] ⑥Salt cleaning agent: formic acid / water (90 / 10, v / v / v), 5mL;

[0045] ⑦ Complex solution 1: Chloroform, 1...

Embodiment 2

[0054] 4) The difference between Example 2 and Example 1 is that 3 mg of ARA bacteria oil is used to replace echium oil as the sample to be tested. The spectrum and peak data of free fatty acid derivatives in ARA bacterial oil are as follows: image 3 As shown, the type of fatty acid can be judged, such as C16:1, C18:1, C18:2, C18:3, C20:1, C20:2, C20:3, C20:4 ( image 3 A); At the same time, for the position of the double bond of unsaturated fatty acids, for example, the derivative product of C18:1 can produce 2 pairs of diagnostic ions (m / z 198.1, 224.1) (m / z 226.1, 252.1), thereby judging that the position of the double bond is C18:1n9,C18:1n7( image 3 B); The derivative product of C18:2 produces two pairs of diagnostic ions (m / z 198.1, 224.1), (m / z 238.1, 264.1), so that it can be judged that the position of the double bond is C18:2n6 image 3 C); The derivative product of C18:3 produces six pairs of diagnostic ions (m / z 156.1, 182.1), (m / z 196.1, 222.1) (m / z 236.1, 272...

Embodiment 3

[0056] The difference between Example 3 and Example 1 is that 3 mg of DHA algae oil is used instead of echium oil as the sample to be tested. Spectrum and peak data of free fatty acid derivatives in DHA algae oil such as Figure 4 As shown, the type of fatty acid can be judged, such as C16:1, C18:1, C18:2, C20:2, C22:1, C22:5C22:6( Figure 4 A); At the same time, for the double bond position of unsaturated fatty acids, for example, the derivative product of C18:1 can generate 2 pairs of diagnostic ions (m / z 198.1, 224.1), thereby judging that the double bond position is C18:1n9( Figure 4 B); The derivative product of C18:2 produces two pairs of diagnostic ions (m / z 198.1, 224.1), (m / z 238.1, 264.1), so that it can be judged that the position of the double bond is C18: 2n6 ( Figure 4 C); The derivative product of C18:3 produces three pairs of diagnostic ions (m / z 156.1, 182.1), (m / z 196.1, 222.1) (m / z 236.1, 272.1), so that it can be judged that the position of the double bo...

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Abstract

The invention discloses a kit for precise structural identification of unsaturated fatty acids in fats and oils, which has the advantages of high specificity, ability to realize precise structural identification, and the like. The kit includes alkali hydrolysis solution, hydrolysis reaction quencher, extract solution, P-B reaction solution, DEEA reactant, salt cleaning agent, complex solution, which uses mass spectrometry to analyze the positions of fatty acids and their double bonds, Improve the quality of food testing. The invention has the advantages of high sensitivity, accurate structure identification, etc., can avoid the operation error caused by the corresponding self-prepared reagents, and can improve the simplicity of operation and the stability of the structure.

Description

technical field [0001] The invention relates to a kit, in particular to a kit for measuring unsaturated fatty acids in the field of agricultural products such as fats and oils or food. Background technique [0002] Edible oils and fats are an important source of dietary lipids and play an important role in various biological functions of humans. They are sources of energy and contain a large amount of essential fatty acids (such as linoleic acid and linolenic acid) and fat-soluble vitamins (A, D , E and K), the main component of triacylglycerols (TAGs) edible oil (accounting for 95%-98%), triglyceride is the main form of fatty acid, the carbon chain length of fatty acid, the number of double bonds and double The cis / trans configuration of the bond has an important influence on the physicochemical and nutritional properties of oil. [0003] The position of the double bond of unsaturated fatty acids is closely related to its function. According to the position of the first do...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 魏芳徐淑玲吕昕董绪燕陈洪黄凤洪
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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