African swine fever virus vaccine

A technology of African swine fever virus and virus particles, applied in the direction of viruses, vaccines, virus peptides, etc.

Pending Publication Date: 2020-03-24
STICHTING WAGENINGEN RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These live attenuated vaccines typically provide up to 100% protection against homologous strains but only partial cross-protection against heterologous strains

Method used

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  • African swine fever virus vaccine
  • African swine fever virus vaccine
  • African swine fever virus vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0108] Example 1: DNA vaccination based on the expression of ASFV T cell epitopes

[0109] method:

[0110] Based on the complete genome sequence of the known ASF isolates and the genome sequence of pigs, the epitope prediction program NetMHCpan is used to provide a list of T cell epitopes independent of the virus strain or pig breed (see Table 1). The 19 highest-ranking T cell epitopes were used to produce a multi-epitope synthetic DNA vaccine. The DNA sequence encodes 19 nonapeptide epitopes separated by 3-5 amino acid spacers containing signals for proteasome cleavage and further processing (see Figure 1A ). Epitope 14 is not used because it is likely to interfere with correct processing. For this purpose, the so-called PADRE universal T cell epitope sequence (see Figure 1B ). To enhance proteasome degradation, a nucleotide sequence for ubiquitin was added to the 5'end of the synthetic gene. This results in more efficient degradation by the proteasome and improved presenta...

Embodiment 2

[0114] Example 2: Vaccination with MVA vector expressing ASFV T cell and B cell epitopes

[0115] method:

[0116] Based on the promising results of the multi-epitope DNA vaccine, the synthetic ASFDVAC2 gene is provided with the MVA 13.5 promoter sequence and inserted into the TK gene of the MVA (attenuated smallpox) vaccine vector by BAC recombination engineering according to the disclosed method (Cottingham, 2012. Methods Mol Biol 890:37-57). The obtained virus was named MVA-VAC2. The insert of MVA-VAC2 includes the left wing of TK, MVA 13.5L promoter, Kozak sequence, synthetic ASFDVAC2 gene, termination sequence from mH5, and right wing of TK starting from the 5'end.

[0117] In addition, using cascade (p30+B602L; p72+A104R) or triple (p54+EP402R+K205R) gene expression cassettes (MVA-p30 / B602L, MVA-p72 / A104R and MVA-p54 / EP402R / K205R, respectively), The genes encoding six well-known major ASFV B cell antigens (p30, p54, p72, EP402R, A104R, and B602L) were inserted into the MVA v...

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Abstract

The invention relates to an African swine fever virus vaccine. To be specific, the invention is directed to a recombinant nucleic acid molecule comprising an expression cassette encoding a polyepitopecomprising T-cell antigens from proteins of African Swine Fever Virus. The invention further relates to a viral particle, comprising said recombinant nucleic acid molecule, and to a viral particle comprising B-cell antigens of African Swine Fever Virus. The invention further relates to methods of stimulating an immune response in a pig comprising administering the recombinant molecule of the invention, and / or the viral particle of the invention, to the pig in an amount effective to induce an immune response.

Description

Technical field [0001] The present invention relates to the field of viruses, and more specifically to the field of African Swine Fever Virus (ASFV). The present invention relates to a method for producing a vaccine against ASFV infection, and to the use of this vaccine to prevent or ameliorate (ameliorate) African swine fever virus infection and / or transmission in pigs. Background technique [0002] African swine fever (ASF) is a viral disease that is only susceptible to pigs and wild boars. After the initial infection, symptoms may take 2 to 10 days to appear. Depending on the pathogenicity of the virus, 30% to 100% of infected animals will die. Common symptoms include: loss of appetite, weakness, redness of the skin, inflammation of the eye mucous membranes, vomiting, bleeding diarrhea, and fever. In addition, the skin may turn blue, the skin in some areas may die (black stains) and bleeding may occur. In addition, diseases can cause spontaneous abortions in pregnant sows....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/34C07K14/005A61K39/12A61P31/20
CPCC07K14/005A61K39/12A61P31/20C12N2710/12022C12N2710/12023C12N2710/12034A61K2039/5258A61K2039/552A61K2039/53C07K2319/40C07K2319/50A61K2039/70C12N2710/24143C12N15/86A61K39/187C12N2710/24043C12N7/00
Inventor 彼得鲁斯·特奥多鲁斯·约翰内森·威廉森贝尔纳杜斯·彼得鲁斯·许贝特斯·佩特斯
Owner STICHTING WAGENINGEN RES
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