A molecular marker method for predicting and identifying chicken belly fat mass and its application
A molecular marker, abdominal fat technology, applied in the field of animal molecular genetics, can solve the problems that have not yet been discovered, and achieve the effect of high accuracy, low cost, and speeding up the breeding process
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[0036] Example 1. Molecular marker method for predicting and identifying chicken belly fat mass.
[0037] 1. Design and synthesis of primers.
[0038] Primers were designed according to the SNP site g.-4354T>A upstream 337bp downstream 27bp sequence of the chicken HBP1 gene, and were synthesized by Jinweizhi Biotechnology Co., Ltd. The primer sequences are as follows:
[0039] HBP1-F:5'-TGTTAATTGCTAAGGGTGGA-3'
[0040] HBP1-R:5'-AGCACAAATAACCAAGAATTACTCG-3'
[0041] 2. Using the amplification primers obtained in step 1 to perform PCR amplification of chicken genomic DNA to obtain amplification products.
[0042] DNA extraction, product amplification:
[0043] 1) Extraction of chicken DNA, the following two methods can be used for the extraction of a small sample of chicken DNA:
[0044] Method 1: (1) Take 20 μl of anticoagulated blood, add 500 μl of poultry lysate, add proteinase K to a final concentration of 100-200 μg / ml, mix and digest at 55°C for 12 hours until there a...
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