Porphyrin-modified cell-penetrating peptide and preparation and application thereof
A technology of penetrating peptides and porphyrins, applied in the field of anti-tumor drug design and synthesis, achieving low cost, good biocompatibility, and favorable effects for industrial production
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[0034] A preparation method of porphyrin-modified penetrating peptide PPA-K6L9 specifically includes the following steps:
[0035] (1) Add 0.0215 mmol pyropheophytin a, 0.0633 mmol, benzotriazole-N,N,N',N'-tetramethylurea hexafluorophosphate (HBTU) to 2 mL N,N- Add 93.3 μL of N,N-diisopropylethylamine (DIEA) to dimethylformamide (DMF), and stir for 30 min at room temperature;
[0036] (2) Add 43.3 mg of N-terminal exposed and C-terminal GGK6L9 penetrating peptide with resin to the reaction system of step (1), and stir overnight at room temperature;
[0037] (3) After the reaction is completed, the remaining reactants are washed with DMF until the supernatant is colorless, and then the DMF is replaced with methanol and then lyophilized;
[0038] (4) Soak the lyophilized solid with 95% trifluoroacetic acid for 4 hours, and then filter to obtain the filtrate; use 10 times the volume of the filtrate to precipitate the solid, and centrifuge to obtain the solid;
[0039] (5) The solid is fre...
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