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Hericium erinaceus polysaccharide extraction and separation method

A technology of Hericium erinaceus polysaccharide and separation method, which is applied in the field of extraction and separation of Hericium erinaceus polysaccharide, can solve the problems of destroying polysaccharide structure, long extraction time, limitation, etc., and achieve the effect of high purity

Active Publication Date: 2020-03-27
福州福德恩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, there are multiple methods for extracting Hericium erinaceus polysaccharides, such as utilizing heat treatment to destroy the cell structure, promoting the release and transfer of intracellular substances, thereby extracting polysaccharides, but the extraction time is long and the extraction rate is low; while acid extraction, Alkaline extraction is a method of extracting polysaccharides by chemically destroying the cell structure. While destroying the cell wall and other structures, it will also destroy the structure of polysaccharides, so it is not commonly used; with the promotion of enzyme preparations, enzymatic hydrolysis is gradually applied to the extraction of polysaccharides , mainly using the enzymatic hydrolysis of pectinase to achieve the purpose of destroying the cell structure, but it is limited due to its high cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Weigh 100g of the fruiting body of Hericium erinaceus, grind the powder through a 80-mesh sieve to obtain Hericium erinaceus powder, put the obtained Hericium erinaceus powder into -180°C to -200°C liquid nitrogen, cool down sharply for 3-5 minutes, and freeze the frozen Hericium erinaceus powder was moved to a high-pressure tank, and 200ml of 1mol / L NaCl solution was added to dissolve and thawed at 30°C. The freezing and thawing process was repeated 3 times, and the high-pressure tank was filled with nitrogen to make the pressure of the high-pressure tank 5500kPa. Dissolve a large amount of nitrogen, open the pressure reducing valve, and reduce the pressure in the tank to normal atmospheric pressure within 1-3 minutes, take out the Hericium erinaceus cell suspension, centrifuge at 5000r / min to get the supernatant, and add 0.8g at a stirring speed of 30r / min / ml chitosan, after 30 minutes of heat preservation in a water bath at 40°C, centrifuge at 5000r / min to get the su...

Embodiment 2

[0032] Weigh 100g of the fruiting body of Hericium erinaceus, grind the powder through a 80-mesh sieve to obtain Hericium erinaceus powder, put the obtained Hericium erinaceus powder into -180°C to -200°C liquid nitrogen, cool down sharply for 3-5 minutes, and freeze the frozen Hericium erinaceus powder was moved to a high-pressure tank, added 200ml of 0.5mol / L sucrose solution to dissolve and thawed at 40°C, the freezing and thawing process was repeated 3 times, and carbon dioxide was filled in the high-pressure tank so that the pressure of the high-pressure tank was 4000kPa, and the cell suspension Dissolve a large amount of carbon dioxide in the tank, open the pressure reducing valve, reduce the pressure in the tank to normal atmospheric pressure within 1-3min, take out the Hericium erinaceus cell suspension, centrifuge at 5000r / min to get the supernatant, and add 2 g / ml ZTC1+1II, keep warm in a water bath at 50°C for 30min, centrifuge at 5000r / min to get the supernatant, an...

Embodiment 3

[0034] Weigh 100g of the fruiting body of Hericium erinaceus, grind the powder through a 80-mesh sieve to obtain Hericium erinaceus powder, put the obtained Hericium erinaceus powder into -180°C to -200°C liquid nitrogen, cool down sharply for 3-5 minutes, and freeze the frozen Hericium erinaceus powder was moved to a high-pressure tank, added 200ml 0.8mol / L NaCl solution to dissolve and thawed at 35°C, the freezing and thawing process was repeated 3 times, and carbon dioxide was filled in the high-pressure tank so that the pressure of the high-pressure tank was 4500kPa, and the cell suspension Dissolve a large amount of carbon dioxide in the tank, open the pressure reducing valve, and reduce the pressure in the tank to normal atmospheric pressure within 1-3 minutes, take out the Hericium erinaceus cell suspension, centrifuge at 5000r / min to get the supernatant, and add 1 g / ml chitosan, keep warm in a water bath at 45°C for 30min, centrifuge at 5000r / min to get the supernatant,...

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Abstract

The invention discloses a hericium erinaceus polysaccharide extraction and separation method, which comprises the following steps: S1, obtaining hericium erinaceus powder; S2, freezing the hericium erinaceus powder with liquid nitrogen, unfreezing the hericium erinaceus powder with an unfreezing agent, and repeatedly freezing and thawing the hericium erinaceus powder for three times; S3, introducing inert gas until the pressure is 4000-5500kpa, and rapidly recovering to normal atmospheric pressure to obtain a hericium erinaceus cell suspension; S4, conducting centrifugal separation, and flocculating supernate through a flocculating agent, thus obtaining a hericium erinaceus polysaccharide extract. Hericium erinaceus polysaccharide is extracted evenly and thoroughly, the extraction speed ishigh, and the operation is easy and convenient. The structural integrity of polysaccharide is protected. The extraction rate is high, and pure physical extraction is achieved. The hericium erinaceuspolysaccharide extract is non-toxic, harmless and low in cost.

Description

technical field [0001] The invention relates to the field of Hericium erinaceus, in particular to a method for extracting and separating polysaccharides from Hericium erinaceus. Background technique [0002] Polysaccharides are the main functional components of Hericium erinaceus, which are water-soluble. There are evidences to prove that the polysaccharides of Hericium erinaceus are mainly composed of glucose, fructose, galactose, mannose, rhamnose, etc. Among them, the content of glucose is the highest, and its structure is mainly composed of The main chain and the branch chain are connected by β(1-3) bonds and β(1-6) bonds, which are natural substances and play an important role in maintaining the life activities of animals and plants. [0003] Hericium erinaceus polysaccharides have many physiological functions, including anti-cancer, treatment of gastritis and gastric ulcer, lowering blood sugar, anti-radiation and improving immunity, scavenging free radicals, anti-agin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/006
Inventor 叶艳鹏方柳慧郑秋芳方成
Owner 福州福德恩生物科技有限公司
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