Housefly acetylcholinesterase gene optimized by codon, protein and applications
A technology for acetylcholinesterase and codon optimization, which can be used in application, genetic engineering, plant genetic improvement and other directions, can solve the problems of low expression efficiency, inability to obtain active recombinase, etc., and achieves improved expression, great application value and production cost. low effect
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[0037] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.
[0038] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.
[0039] A codon-optimized housefly acetylcholinesterase gene, the nucleotide sequence of the housefly acetylcholinesterase gene is SEQ ID NO.3.
[0040] Preferably, the housefly acetylcholinesterase gene is codon-optimized on the basis of the existing housefly acetylcholinesterase gene, which can encode a protein with acetylcholinesterase activity.
[0041] Preferably, the housefly acetylcholinesterase gene is obtained by optimizing 29 codons on the basis of the original gene sequence SEQ ID NO.1, namely ccg→cca cgc, c...
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