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Housefly acetylcholinesterase gene optimized by codon, protein and applications

A technology for acetylcholinesterase and codon optimization, which can be used in application, genetic engineering, plant genetic improvement and other directions, can solve the problems of low expression efficiency, inability to obtain active recombinase, etc., and achieves improved expression, great application value and production cost. low effect

Inactive Publication Date: 2020-03-27
TIANJIN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the expression of prokaryotic system cannot obtain active recombinant enzymes, and the expression efficiency of eukaryotic system is low.

Method used

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  • Housefly acetylcholinesterase gene optimized by codon, protein and applications
  • Housefly acetylcholinesterase gene optimized by codon, protein and applications
  • Housefly acetylcholinesterase gene optimized by codon, protein and applications

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Embodiment Construction

[0037] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0038] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0039] A codon-optimized housefly acetylcholinesterase gene, the nucleotide sequence of the housefly acetylcholinesterase gene is SEQ ID NO.3.

[0040] Preferably, the housefly acetylcholinesterase gene is codon-optimized on the basis of the existing housefly acetylcholinesterase gene, which can encode a protein with acetylcholinesterase activity.

[0041] Preferably, the housefly acetylcholinesterase gene is obtained by optimizing 29 codons on the basis of the original gene sequence SEQ ID NO.1, namely ccg→cca cgc, c...

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Abstract

The invention relates to a housefly acetylcholinesterase gene optimized by codon. The nucleotide sequence of the gene is SEQ ID NO. 3. The optimized gene has significant sensibility on organophosphorus and aminocaproate pesticides with low concentrations and can be used for the pesticide residue detection of fruits and vegetables; and the gene is simple in operation and low in production cost, andcan provide a stable and sensitive protease material for the developing of pesticide residue detection products. The sequence is beneficial to massively express housefly acetylcholinesterase throughgenetic engineering, and an encoded amino acid sequence cannot be changed, so that the gene can be more stable in enzymatic property and close to bio-extracted acetylcholinesterase; and the gene provides convenience for cost reducing and wide application, and has great application values.

Description

technical field [0001] The invention belongs to the technical field of genetic bioengineering, in particular to a codon-optimized gene, protein and application of housefly acetylcholinesterase. Background technique [0002] Acetylcholinesterase is a key enzyme in biological nerve transmission, which can degrade acetylcholine, terminate the excitatory effect of neurotransmitters on postsynaptic membranes, and ensure the normal transmission of nerve signals in organisms. [0003] Studies have shown that a variety of compounds can inhibit the activity of acetylcholinesterase, among which organophosphorus and carbamate pesticides have obvious inhibitory effects. Organophosphorus pesticides are the most widely used pesticides in my country. Excessive pesticide residues in fruits and vegetables can easily cause acute organophosphorus pesticide poisoning, further causing heart damage and even sudden cardiac death. As a big country in the production and consumption of fruits and ve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/55C12N9/18C12N15/81C12N1/19C12Q1/46C12R1/84
CPCC12N9/18C12N15/815C12Q1/46C12Y301/01007C12N2800/22G01N2333/918
Inventor 杜欣军贾起起李萍杜婷王硕
Owner TIANJIN UNIV OF SCI & TECH
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