Triple test strip for detecting methamphetamine, morphine and ketamine as well as preparation method and application method of triple test strip

Abstract

The invention relates to a triple test strip for detecting methamphetamine, morphine and ketamine as well as a preparation method and an application method of the triple test strip. The test strip sequentially comprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate, a methamphetamine antibody, a morphine antibody and a ketamine antibody marked by the time-resolved fluorescent microspheres are sprayed on the conjugate release pad, and the reaction membrane is coated with a detection line T1 of a methylamphetamine antigen, a detection line T2 of a morphine antigen, a detection line T3 of a ketamine antigen and a quality control line C of a goat anti-mouse secondary antibody or Biotin-BSA. According to the immunochromatographytechnology, the simultaneous and rapid immunoassay of three drugs including methamphetamine, morphine and ketamine is realized. The invention also provides a method for simultaneously detecting the methamphetamine, morphine and ketamine in a sample by using the above test strip. The triple test strip and the method have the advantages of being high in sensitivity and rapid in detection, being capable of achieving accurate quantification, being convenient to operate, and being capable of achieving the rapid detection and on-site detection on a large batch of samples.

Description

technical field [0001] The invention relates to the detection of drugs such as methamphetamine, morphine and ketamine, in particular to a triple test strip for simultaneous quantitative detection of methamphetamine, morphine and ketamine by using time-resolved fluorescent microsphere immunochromatography technology and its preparation method and application method. Background technique [0002] Methamphetamine, commonly known as "ice", is a stimulant psychotropic drug with a strong central nervous system excitatory effect. It is a class of psychotropic drugs and narcotic drugs strictly controlled by the state. At present, the abuse of amphetamine-like substances in the world is on the rise. Experts predict that amphetamine-type stimulants will become one of the most widely abused drugs of the 21st century. [0003] As criminal cases related to methamphetamine increase year by year and the country's management of methamphetamine becomes increasingly strict, this field has p...

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to overcome the shortcomings of the existing methods for detecting methamphetamine, morphine and ketamine, which are complex in operation, time-consuming, unable to realize rapid detection of large batches of samples, and unable to detect multiple drugs at the same time, Provide a triple test strip for detecting drugs with simple operation, high sensitivity, fast detection speed and low cost, its preparation method and application method, so as to realize rapid detection and on-site monitoring of multiple drugs at the same time for a large number of samples

Images