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Lactobacillus plantarum proliferation agent and applications thereof

A technology of Lactobacillus plantarum and a proliferating agent, applied in the field of microorganisms, can solve problems such as no growth, achieve the effects of low cost, increase application potential, and enhance health care and probiotic effects

Inactive Publication Date: 2020-04-03
南昌诺汇医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is generally accepted that Lactobacillus plantarum is very difficult to grow in milk, with little growth

Method used

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  • Lactobacillus plantarum proliferation agent and applications thereof
  • Lactobacillus plantarum proliferation agent and applications thereof
  • Lactobacillus plantarum proliferation agent and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The following experimental groups were set up, and the average of 5 repetitions in each group was taken.

[0034] Group 1:

[0035] A plant lactobacillus proliferator, which is:

[0036] Bacillus subtilis CGMCC 18057 fermentation supernatant.

[0037] Preferably, the fermentation supernatant is obtained by fermentation in a medium containing soybean components.

[0038] As a further preference, the preparation method of the fermentation supernatant is:

[0039] (1) Activated strains:

[0040] A ring of Bacillus subtilis CGMCC 18057 was inoculated into LB solid medium to streak, and cultured aerobically at 37°C for 24 hours. Pick a single colony with better growth in the plate and inoculate it into LB liquid medium for activation, and finally adjust the concentration to 10 8 cfu / ml Bacillus subtilis seed solution.

[0041] (2) According to the inoculum amount of 3% by volume, the seed solution was inoculated into the fermentation medium for fermentation, the cultur...

Embodiment 2

[0059] Determination of its growth-promoting effect on Lactobacillus plantarum ST-III in milk, it specifically comprises the steps:

[0060] (1) Preparation of Lactobacillus plantarum ST-III seed liquid: Take a ring of pure Lactobacillus and inoculate it into MRS solid medium to streak, and culture anaerobically at 37°C for 24 hours. Pick a single colony with good growth in the plate and inoculate it into MRS liquid medium for activation, and culture it anaerobically to 10 8 cfu / ml Lactobacillus plantarum seed solution. ;

[0061] (2) Take the proliferating agents of groups 1-4 and dissolve them in water to prepare a solution with a concentration of 10%;

[0062](3) Inoculate the Lactobacillus plantarum seed solution prepared in step (1) into sterilized raw milk at a ratio of 2% (v / v), and add the Lactobacillus plantarum obtained in step (2) to proliferate by adding 5% of the volume of raw milk agent, mix evenly, and let it stand for cultivation.

[0063] Preferably, cultu...

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Abstract

The invention discloses a lactobacillus plantarum proliferation agent and applications thereof. The proliferation agent is the fermentation supernatant of B.subtilis sp CGMCC 18057; 6'-acetyl ginsenoside F1 is also included; and the fermentation supernatant is obtained in a culture medium containing soybean components through fermentation. The proliferation agent is simple in preparation method and low in cost; the used strain of the proliferation agent is probiotics, so that other health probiotic effects can be enhanced; and the prepared proliferation agent has significant promotion effectson the growth of lactobacillus plantarum in milk, so that the application space and application potential of the lactobacillus plantarum in actual product development can be greatly enhanced.

Description

technical field [0001] The invention relates to a proliferating agent for plant lactobacillus, which belongs to the technical field of microorganisms. Background technique [0002] Lactobacillus plantarum is a kind of lactic acid bacteria, the optimum growth temperature is 30-35°C, anaerobic or facultative anaerobic, the strain is straight or curved rod-shaped, single, sometimes in pairs or chains, the optimum pH is 6.5 Left and right, belong to homofermentative lactic acid bacteria. The difference between this bacterium and other lactic acid bacteria is that the number of viable bacteria of this bacterium is relatively high, and it can produce a large amount of acid, so that the pH value of the water is stable and does not rise, and the acidic substances produced by it can degrade heavy metals; because this bacterium is anaerobic Bacteria (facultative aerobic), can produce unique lactobacillus in the process of reproduction, and lactobacillus is a kind of biological preser...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/38C12R1/125
CPCC12N1/20C12N1/38
Inventor 张楠叶水文
Owner 南昌诺汇医药科技有限公司
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