Fluorescent microsphere co-doped with multiple near-infrared-II fluorescent dyes, preparation method and applications thereof

A technology of fluorescent dyes and fluorescent microspheres, which is applied in the direction of luminescent materials, analytical materials, material inspection products, etc., can solve the problems of reducing detection sensitivity and accuracy, fluorescence quenching, and reducing fluorescence intensity, so as to avoid fluorescence quenching, Good stability, increase the effect of fluorescence intensity

Pending Publication Date: 2020-04-10
FUDAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the fluorescent dyes used contain heavy atoms, which are easy to cause fluorescence quenching, and embedding the same dye molecule with a planar configuration is easy to form H aggregation and J aggregation. The two aggregation states can significantly reduce the fluorescence intensity, thereby reducing detection. Sensitivity and Accuracy

Method used

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  • Fluorescent microsphere co-doped with multiple near-infrared-II fluorescent dyes, preparation method and applications thereof
  • Fluorescent microsphere co-doped with multiple near-infrared-II fluorescent dyes, preparation method and applications thereof
  • Fluorescent microsphere co-doped with multiple near-infrared-II fluorescent dyes, preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Example 1: Preparation of fluorescent microspheres co-doped with two near-infrared II region fluorescent dyes

[0074] Two kinds of near-infrared II region fluorescent dyes are co-doped with fluorescent microspheres, including carboxypolystyrene spheres and two near-infrared II region fluorescent dyes embedded in the carboxypolystyrene spheres.

[0075] The particle size of the carboxylated polystyrene spheres is 300 nm.

[0076] The two near-infrared II region fluorescent dyes include Fluorochrome A and Fluorochrome B.

[0077] Fluorescent dye A, the structure is as follows:

[0078]

[0079] Fluorescent dye B, the structure is as follows:

[0080]

[0081] The specific preparation steps of co-doped fluorescent microspheres with two kinds of near-infrared II fluorescent dyes are as follows:

[0082] 1. Disperse 30mg of mixed fluorescent dye (fluorescent dye A: fluorescent dye B = 3:7, 4:6, 5:5, 6:4 or 7:3, W / W) in 10mL tetrahydrofuran solution to form organic ...

Embodiment 2

[0109] Example 2: Application of two kinds of near-infrared II region fluorescent dyes co-doped with fluorescent microspheres

[0110] Two kinds of near-infrared region II fluorescent dyes co-doped with fluorescent microspheres prepared in Example 1 were applied to immunochromatographic test strips to detect procalcitonin (PCT) in whole blood.

[0111] 1. Separate the detection antibody PCT-Ab 1 Fluorescent probes prepared by co-doping fluorescent microspheres with two near-infrared II region fluorescent dyes and embedding a near-infrared II region fluorescent dye fluorescent microspheres

[0112]1) Take 100 mg of fluorescent microspheres embedded with 30 mg of mixed fluorescent dye (fluorescent dye A: fluorescent dye B = 6:4, W / W) in Example 1, fluorescent microspheres embedded with 30 mg of fluorescent dye A in Comparative Example 1, and Comparative Example 1 Fluorescent microspheres embedded with 30 mg of fluorescent dye B were centrifuged, redissolved in 18 mL of BBS buff...

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Abstract

The invention discloses a fluorescent microsphere co-doped with multiple near-infrared-II fluorescent dyes. The fluorescent microsphere comprises a macromolecule polymer microsphere and multiple near-infrared-II fluorescent dyes embedded in the macromolecule polymer microsphere. The invention also discloses a preparation method of the fluorescent microsphere. According to the invention, two or more near-infrared-II fluorescent dyes are embedded in a macromolecule polymer microsphere, and the ordered arrangement among molecules is reduced through different dye structures and mutual doping of molecular weights, so that the H aggregation and the J aggregation are further remarkably reduced, and the fluorescence intensity is enhanced so as to improve the detection sensitivity and the detectionaccuracy; and with the application of a fluorescent probe obtained by covalently coupling the fluorescent microsphere and a detection antibody in an immunodetection technology, the application prospect is wide.

Description

technical field [0001] The invention belongs to the technical field of nano-medicine biotechnology detection, and specifically relates to co-doped fluorescent microspheres with various near-infrared II region fluorescent dyes, a preparation method thereof, and an application in immunochromatographic detection. Background technique [0002] The fluorescent immunochromatographic test strip technology based on fluorescent microspheres is based on the specific recognition between antigens and antibodies, using fluorescent microspheres as signal labels, and establishing a one-to-one correspondence between the fluorescent signal and the concentration of the target analyte, so as to realize the It is a means to achieve accurate and rapid quantification of target analytes in samples, and is widely used in the field of clinical medicine. [0003] Fluorescent microspheres currently used in fluorescent immunochromatography test strip technology are mainly fluorescent microspheres prepa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/06G01N33/558G01N33/58
CPCC09K11/025C09K11/06G01N33/582G01N33/587G01N33/558C09K2211/1081C09K2211/1092C09K2211/1007C09K2211/1014C09K2211/1011
Inventor 李富友陈锐周晓波吴勇
Owner FUDAN UNIV
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