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Enteric microorganism for diagnosing cardiac valve calcification, primer group and application thereof

A technology of intestinal microbes and heart valves, which is applied in the diagnosis of intestinal microbes for heart valve calcification, primer sets and application fields, can solve problems such as diseases that are easily overlooked by people, patients with valvular stenosis, heart failure, etc., and achieve a simple and fast diagnosis process , High diagnostic specificity, non-invasive cost effect

Pending Publication Date: 2020-04-17
NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] Currently, the means for diagnosing valve calcification mainly include echocardiography and cardiac CT. Severe valvular stenosis and heart failure have occurred; in addition, the current uneven distribution of medical resources and the lack of medical resources, patients need to wait in line for a long time to see a doctor or complete expensive imaging examinations, due to the pressure of the current fast pace of life , it is difficult for ordinary people to spare a lot of time for health screening, which makes the disease easy to be ignored by people

Method used

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  • Enteric microorganism for diagnosing cardiac valve calcification, primer group and application thereof
  • Enteric microorganism for diagnosing cardiac valve calcification, primer group and application thereof
  • Enteric microorganism for diagnosing cardiac valve calcification, primer group and application thereof

Examples

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Embodiment

[0038] This example was carried out in Nanfang Hospital from April 2017 to February 2018. The feces of the healthy control group and the patients with heart valve calcification were collected respectively. The collected feces were mid-section feces, and the collection volume was 200 mg, which was stored at -80°C In the refrigerator. The subjects whose samples were collected had not taken antibiotics or probiotic products 30 days before the sample collection, had not suffered from high blood pressure, had never taken drugs that may interfere with sugar and lipid metabolism, and had not combined diseases that affected the intestinal microecology.

[0039] In this example, bacterial genomic DNA in feces was extracted using a bacterial genome total DNA extraction kit, the concentration of DNA was measured by a spectrophotometer, and the integrity of the DNA was detected by electrophoresis, followed by ddH 2 0 DNA was diluted to 10 ng / μL for construction of 16S amplified library. ...

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Abstract

The invention relates to the technical field of auxiliary diagnosis of diseases, and discloses an enteric microorganism for diagnosing cardiac valve calcification, which comprises one or more of Verrucomicrobiaceae or Prevotella or Bacteroides or Veillonella. The invention further discloses an application of the enteric microorganism for diagnosing cardiac valve calcification in preparation of a kit for diagnosing the cardiac valve calcification. The invention also discloses an application of the enteric microorganism for diagnosing cardiac valve calcification in preparing a chip for diagnosing cardiac valve calcification. The invention also discloses a primer group for detecting the enteric microorganisms. According to the invention, the level change of Verrucomicrobiaceae or Prevotella or Bacteroides or Veillonella in intestinal flora in excrement can be used for diagnosing the cardiac valve calcification; rapid diagnosis of the cardiac valve calcification is achieved, diagnosis is easy, convenient and rapid, invasion is avoided, cost is low, diagnosis specificity is high, sensitivity is high, an application object is suitable for large-scale crowd screening, and final monitoringcan be achieved for individuals.

Description

technical field [0001] The invention relates to the technical field of aided diagnosis of diseases, in particular to intestinal microorganisms, primer sets and applications for diagnosing heart valve calcification. Background technique [0002] With the development of population aging process, the incidence of heart valve calcification is increasing year by year, seriously endangering people's health. Heart valve calcification mainly refers to a kind of valve aging, calcification and degenerative changes with age, also known as degenerative heart valve disease. Some scholars believe that valvular calcification is a kind of advanced atherosclerosis. From the perspective of anatomy, it can also be considered that the heart valve is the distal end of the blood vessel. Atherosclerosis and valvular calcification have a very similar pathophysiological basis. Coronary heart disease is a coronary atherosclerotic disease, and patients with pathophysiological basis of coronary heart ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/04C12N15/11C12R1/01
CPCC12Q1/689G01N2800/323Y02A50/30
Inventor 曾庆春许顶立刘祖恒李加英詹琼谭燕赖文岩白煜佳张振
Owner NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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