Methods for detecting cancers, and detection reagent

A cancer and reagent technology, applied in the field of cancer detection and detection reagents, can solve problems such as unclear disease effects, and achieve high-precision results

Pending Publication Date: 2020-04-21
TOSOH CORP
View PDF23 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the effect of using this protein as an assay object to detect diseases is unclear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods for detecting cancers, and detection reagent
  • Methods for detecting cancers, and detection reagent
  • Methods for detecting cancers, and detection reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] Preparation of monoclonal antibody

[0107] Five types of monoclonal antibodies that recognize the GDF15 propeptide were produced by a known method (DNA immunization: JP-A-2013-061321).

Embodiment 2

[0108] Epitope Analysis of Various Monoclonal Antibodies

[0109] The antigen recognition site of each antibody produced in Example 1 was identified from cell culture supernatants expressing the complete GDF15 propeptide (iGDPP) and N-terminal deleted GDF15 propeptide fragment (dNT-GDPP) variants.

[0110] In order to perform expression evaluation and purification procedures of various recombinant GDPPs, a FLAG tag and a StrepII-tag were further inserted at the 5' end, and a coding sequence consisting of 7 amino acids on the C-terminal side of BNP (brain natriuretic peptide) was inserted at the 3' end. A plasmid capable of expressing secreted iGDPP and four types of dNT-GDPP, which is an oligonucleotide of the BNC peptide (JP-A-2009-240300). The structures of various recombinant GDPPs produced are shown in figure 1 , the specific preparation method is shown below.

[0111] It should be noted that, if figure 1 As shown, in the amino acid sequence of SEQ ID NO: 2, iGDPP co...

Embodiment 3

[0128] Preparation of GDF15 Propeptide Determination Reagent

[0129]Using the anti-GDPP monoclonal antibody prepared in Example 1, two kinds of GDPP measurement reagents were prepared by changing the combination of antibodies. One is a combination of an antibody that recognizes the N-terminal region of GDPP (TS-GDPP02) and an antibody that recognizes the C-terminal region (TS-GDPP04), and detects intact GDPP (iGDPP). Another detects both iGDPP and dNT-GDPP by a combination between antibodies recognizing the C-terminal region (TS-GDPP04 and TS-GDPP08). The value detected by the latter was taken as total GDPP (tGDPP). The specific preparation method is described below.

[0130] (1) Make the anti-GDF15 propeptide monoclonal antibody (TS-GDPP02 and 08) physically adsorb on the water-insoluble ferrite carrier at room temperature day and night in the form of 100ng / carrier, and then buffer it with 100mM Tris containing 1%BSA solution (pH 8.0) for blocking at 40°C for 4 hours to ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Login to view more

Abstract

The present invention provides methods for detecting cancers simply and with high precision, and a reagent that can be used for the methods. According to the present invention, a cancer (excluding a castration-resistant prostate cancer) is detected by measuring the intact growth differentiation factor (GDF15) propeptide level, the GDF15 propeptide fragment level, or the total of the intact GDF15 propetide level and the GDF15 propetide fragment level in a sample. The abovementioned methods for detecting cancer include methods for detecting gastric cancer, pancreatic cancer, colorectal cancer, lung cancer, breast cancer or esophageal cancer, or methods for differentiating and detecting non-small cell lung cancer and small cell lung cancer. In addition, antibodies that specifically recognizeGDF15 propeptides are included in a reagent for detecting cancers.

Description

technical field [0001] The present invention relates to a propeptide of a Growth and Differentiation Factor 15 (Growth and Differentiation Factor 15, hereinafter also referred to as "GDF15") protein in blood, its decomposition product, and a cancer detection method and detection reagent for measuring them. Background technique [0002] Tumor markers for detecting cancer generally include those shown in Table 1. However, the positive rates of all markers in the early stage of cancer are low, and there are many problems such as false positives in benign tumors and inflammation, and non-detection of highly malignant cancers. Therefore, the discovery of tumor markers and the development of assays capable of detecting these cancers with high accuracy are expected. [0003] [Table 1] [0004] [0005] Growth differentiation factor 15 (GDF15) is the same as macrophage inhibitory factor 1 (Macrophage Inhibitory Cytokine 1: MIC-1), nonsteroidal anti-inflammatory drug-activated g...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68C07K14/475C07K16/22G01N27/62G01N33/574C12P21/08
CPCC07K14/475C07K16/22G01N33/53G01N33/574G01N33/57488G01N2333/495G01N33/6848
Inventor 明庭昇平大竹则久
Owner TOSOH CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap