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YAP1 gene modified mesenchymal stem cells and preparation method thereof

A technology of stromal stem cells and gene modification, applied in the field of YAP1 gene-modified mesenchymal stem cells and their preparation, can solve the problems of long time and slow proliferation rate, and achieve the effect of promoting proliferation and increasing yield

Inactive Publication Date: 2020-04-28
ZHEJIANG UNIV
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

The proliferation rate of mesenchymal stem cells without genetic modification is slow in vitro, and it takes a long time to expand to the order of clinical application

Method used

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  • YAP1 gene modified mesenchymal stem cells and preparation method thereof
  • YAP1 gene modified mesenchymal stem cells and preparation method thereof
  • YAP1 gene modified mesenchymal stem cells and preparation method thereof

Examples

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Embodiment Construction

[0027] The source of the mesenchymal stem cells used in the present invention is explained: various human tissues used in the present invention are clinical waste or isolated human tissues. For example, the placenta in the examples was obtained from the Obstetrics and Gynecology Ward of the First Affiliated Hospital of Zhejiang University School of Medicine, and all protocols for human tissue and cell processing were approved by the Hospital Research Ethics Committee (ethics number: 2013-272). The technical solution of the present invention does not involve the specific operation of the process of obtaining human tissue.

[0028] Unless otherwise specified, the biochemical reagents used in the examples are all commercially available reagents, and the technical means used in the examples are conventional means well known to those skilled in the art.

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Abstract

The invention relates to the technical field of biology, and aims to provide YAP1 gene modified mesenchymal stem cells and a preparation method thereof. The mesenchymal stem cells are primary mesenchymal stem cells modified with overexpressed YAP1 genes, wherein the YAP1 genes are from YAP1 lentiviral vectors or YAP1 plasmid vectors, and the primary mesenchymal stem cells are from any of the following human tissue of placenta, umbilical cords or adipose tissue. The obtained YAP1 gene modified mesenchymal stem cells do not influence the phenotype and the disintegration capacity of MSC; throughthe mesenchymal stem cells modified with overexpressed YAP1 genes, proliferation of the mesenchymal stem cells can be notably promoted, and the yield of cells can be further increased; and therefore,massive mesenchymal stem cells can be quickly obtained for clinical stem cell transplantation treatment.

Description

[0001] field of invention [0002] The invention belongs to the field of biotechnology, and in particular relates to a YAP1 gene-modified mesenchymal stem cell and a preparation method thereof. Background technique [0003] In regenerative medicine and clinical therapy, mesenchymal stem cells (MSCs) have become the most promising substitute for embryonic stem cells due to their self-renewal and multilineage differentiation capabilities. In recent years, successive preclinical and clinical studies have proved that mesenchymal stem cells have obvious therapeutic effects on immune diseases, myocardial injury, liver disease, lung injury, kidney disease and diabetes. MSCs can be isolated from a variety of tissues, such as: bone marrow, placenta, adipose tissue, synovial tissue, lung tissue, umbilical cord blood, peripheral blood, etc. However, previous studies have shown that regardless of the source of mesenchymal stem cells, the optimal number of cells for a single implantation ...

Claims

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Application Information

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IPC IPC(8): C12N5/10C12N15/867C12N15/85C12N5/0775
CPCC12N5/0667C12N5/0668C12N15/86C12N15/85C12N2740/15043C12N2800/107C12N2509/00C12N2510/00C12N2740/16043C12N5/0605
Inventor 曹红翠冯旭东俞炯潘巧玲杨金凤
Owner ZHEJIANG UNIV
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