A fluorescent probe for detecting superoxide anion free radicals in peroxisomes and its application

A technology of peroxisome and superoxide anion is applied in the field of detection and analysis to achieve the effects of good selectivity, low cytotoxicity and sensitive detection

Active Publication Date: 2021-08-17
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, currently used to detect O in peroxisomes 2 ·- tool has not been reported yet

Method used

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  • A fluorescent probe for detecting superoxide anion free radicals in peroxisomes and its application
  • A fluorescent probe for detecting superoxide anion free radicals in peroxisomes and its application
  • A fluorescent probe for detecting superoxide anion free radicals in peroxisomes and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0042] A fluorescent probe, the fluorescent probe has the following structure:

[0043]

[0044] Effect verification:

[0045] Optical Properties of Examples of the Invention. Join O 2 ·- After that, the probe (TCP) showed an absorption peak at about 370 nm. ( figure 1 A). like figure 1 As shown in B, under single-photon 370nm excitation, the fluorescence intensity of TCP at 495nm is weak, while TCP with 20μMO 2 ·- After incubation together, the fluorescence intensity of the probes at 495nm was significantly enhanced, and the fluorescence intensity was 4 times of the initial fluorescence intensity. like figure 1 As shown in C, the fluorescence intensity of TCP at 495 nm is enhanced by more than 8 times under two-photon 800 nm excitation.

[0046] Experiments of the embodiments of the present invention in cells. We first used 0.1 μg / mL 2-Me (to make cells oxidatively stress produce O 2 ·- ) to incubate the PC12 cells for 15 min, then add TCP (20 μM) and incubate...

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Abstract

The invention provides a fluorescent probe for detecting superoxide anion free radicals in peroxisomes and an application thereof, belonging to the technical field of detection and analysis. The present invention designs a kind of with caffeic acid as O 2 ·‑ The recognition group and fluorophore, the fluorescent probe for detecting superoxide anion free radical with small peptide (QSKL) as the targeting group of peroxisome, its structural formula is as follows: This fluorescent probe is especially suitable for The detection of superoxide anion free radicals at the level of cell structure, especially at the level of subcellular structure (such as peroxisome) has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of detection and analysis, in particular to a fluorescent probe for detecting superoxide anion free radicals in peroxisomes and an application thereof. Background technique [0002] The information disclosed in the Background of the Invention is only intended to increase the understanding of the general background of the invention, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes the prior art that is already known to those skilled in the art. [0003] Depression is a global mental disease with high prevalence, relapse rate, mortality rate and disability rate, which seriously endangers human health. Unfortunately, the current understanding of the pathophysiology of depression is still very imperfect. In order to effectively prevent and treat depression, we must have a thorough understanding of the occurrence and development of depression. Tw...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/083C09K11/06G01N33/68G01N21/64
CPCC07K5/0806C09K11/06C09K2211/1007G01N21/6428G01N33/68G01N2410/00
Inventor 唐波丁琪李平王昕
Owner SHANDONG NORMAL UNIV
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