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Method of extraction and flaking of body fluid cells for checking bladder cancer

An extraction method and body fluid cell technology, which are applied in the field of body fluid cell extraction and production, can solve the problems of insufficient turnaround time for inspection, short storage time of urine cells, low detection rate, etc., and solve the problem of insufficient turnaround time for inspection. , rich diagnostic information, and the effect of increasing the number of samples

Pending Publication Date: 2020-05-19
HUBEI TAIKANG MEDICAL EQUIP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, urine samples are collected for three days in clinical practice and tested three times in a row. The detection rate is low, and the number of exfoliated cells in the urine sample is small, which makes it difficult to diagnose. , cannot really benefit the public

Method used

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  • Method of extraction and flaking of body fluid cells for checking bladder cancer
  • Method of extraction and flaking of body fluid cells for checking bladder cancer
  • Method of extraction and flaking of body fluid cells for checking bladder cancer

Examples

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Comparison scheme
Effect test

Embodiment 1

[0196] Embodiment 1 provides a method for extracting body fluid cells for checking bladder cancer, the extraction method comprising: a sampling process and a treatment process;

[0197] The steps of the sampling process specifically include:

[0198] (1) Open the package, take out the collection cup, and collect 50mL of urine;

[0199] (2) Precipitate the urine for 30 minutes, remove 5mL of the supernatant, and pour 40mL of the middle urine into the specimen collection bottle;

[0200] (3) Tighten the cap of the urine collection liquid collection bottle, shake the bottom liquid until it is evenly mixed, and mark it to obtain the urine collection liquid;

[0201] The urine is the first morning urine;

[0202] The sample collection bottle contains 1mL of cell-protecting urine sample solution; the cell-protecting urine sample solution, in parts by weight, includes 50 parts of alcohol, 15 parts of bactericide, 5 parts of preservative, and 20 parts of deionized water ;

[0203]...

Embodiment 2

[0226] Embodiment 2 provides a method for extracting body fluid cells for checking bladder cancer, the extraction method includes: a sampling process and a processing process;

[0227] The steps of the sampling process specifically include:

[0228] (1) Open the package, take out the collection cup, and collect 500mL of urine;

[0229] (2) Precipitate the urine for 10 minutes, remove 280mL of the supernatant, and pour 200mL of the middle urine into the specimen collection bottle;

[0230] (3) Tighten the cap of the urine collection liquid collection bottle, shake the bottom liquid until it is evenly mixed, and mark it to obtain the urine collection liquid;

[0231] The urine is the first morning urine;

[0232] The sample collection bottle contains 35mL of cell-protecting urine sample solution; the cell-protecting urine sample solution, in parts by weight, includes 80 parts of alcohol, 15 parts of bactericide, 5 parts of preservative, and 20 parts of deionized water ;

[0...

Embodiment 3

[0248] Embodiment 3 provides a method for extracting body fluid cells for checking bladder cancer, the extraction method comprising: a sampling process and a processing process;

[0249] The steps of the sampling process specifically include:

[0250] (1) Open the package, take out the collection cup, and collect 300mL of urine;

[0251] (2) Precipitate the urine for 15 minutes, remove 200mL of the supernatant, and pour 90mL of the middle urine into the specimen collection bottle;

[0252] (3) Tighten the cap of the urine collection liquid collection bottle, shake the bottom liquid until it is evenly mixed, and mark it to obtain the urine collection liquid;

[0253] The urine is the first morning urine;

[0254] The sample collection bottle contains 10 mL of cell-protecting urine sample solution; the cell-protecting urine sample solution, in parts by weight, includes 60 parts of alcohol, 25 parts of bactericide, 10 parts of preservative, and 30 parts of deionized water ;

...

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Abstract

The invention relates to the field of cell pathology detection, in particular to a method of extraction and flaking of body fluid cells for checking bladder cancer. The extraction method comprises a sampling process and a treatment process. According to the method of extraction of body fluid cells for checking bladder cancer, urine sediment is broken and decomposed without damage, the problems that urine cells cannot be stored for a long time and the quantity of the cells is small due to the fact that the urine is autolyzed after leaving the human body for 2 hours are solved, the urine samplecan be stored for 7-10 days, the problem that the inspection turnover time is not enough is thoroughly solved, and the non-invasive detection can really benefit the public. The first morning urine with the most abundant diagnostic information and the most urinary sediments is adopted so that exfoliated cells can be completely dissociated from the urinary sediments, more than 5000 cells can be collected from the first morning urine and the sampling quantity, quality and detection success rate are greatly improved.

Description

technical field [0001] The invention relates to the field of cytopathological detection, in particular to a method for extracting and preparing slices of body fluid cells for checking bladder cancer. Background technique [0002] Bladder cancer refers to malignant tumors that occur on the bladder mucosa. It is the most common malignant tumor of the urinary system and one of the ten most common tumors in the whole body. It accounts for the first place in the incidence of urogenital tumors in my country, and ranks second in the West after prostate cancer. In 2012, the incidence rate of bladder cancer in the national tumor registration area was 6.61 / 100,000, ranking ninth in the incidence rate of malignant tumors. Bladder cancer can occur at any age, even in children. Its incidence rate increases with age, and the peak age is 50-70 years old. The incidence of bladder cancer in men is 3-4 times that of women. About 90% of bladder cancer patients initially present with hemat...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30A61B10/00A01N1/02
CPCG01N1/2813G01N1/30A61B10/007A01N1/0226
Inventor 肖国林于昆雪
Owner HUBEI TAIKANG MEDICAL EQUIP
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