Diode array detector based on multistage signal calibration and calibration method

Abstract

The invention relates to a detection technology of a liquid chromatography system. The invention relates to a diode array detector, in particular to a diode array detector based on multistage signal calibration and a calibration method. Dark current calibration is realized by utilizing automatic control of the optical path switching mechanism and the blackening array unit, and an internal reference function of the signal collection circuit is utilized. According to the invention, the collected spectral signals are calibrated in real time, the problems of high noise and drift signals of a diodearray detector instrument and low accuracy of a measurement result are solved, real-time calibration of the spectral signals of the instrument is realized, and the accuracy of the detection result isimproved.

Description

technical field [0001] The invention relates to the detection technology of a liquid chromatography system, in particular to a diode array detector and a calibration method based on multilevel signal calibration. Background technique [0002] For liquid chromatography analysis, most of the measured substances have ultraviolet absorption, so the light absorption ultraviolet detector based on Lambert Beer's law is one of the most widely used selective detectors in liquid chromatography systems. From the perspective of the optical path structure, according to the relative position of the spectroscopic device and the sample cell, this type of detector can be divided into ultraviolet-visible detectors and diode array detectors. The former first splits the compound light, and irradiates the sample with monochromatic light to obtain the absorbance information of the sample at a certain wavelength; the latter uses the compound light to irradiate the sample, and splits the transmitte...

AI Technical Summary

Problems solved by technology

[0008] Aiming at the problems existing in the above-mentioned prior art, the present invention discloses a diode array detector and a calibration method based on multi-level signal calibration. The present invention utilizes the automatic control of the optical path switch mechanism and the blackening array unit to realize dark current calibration and utilizes The internal reference function of the signal acquisition circuit performs real-time calibration of the collected spectral signals, which solves the problems of high noise and drift signals of the diode array detector instrument and low accuracy of measurement results, and realizes real-time calibration of the spectral signals of the instrument , which improves the accuracy of the detection results

Images