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Extraction method of grape seed extract with high procyanidine content

A proanthocyanidin and extraction method technology, applied in the direction of organic chemistry, can solve the problems of low extraction efficiency and waste, and achieve the effect of improving extraction capacity, increasing yield, and good extraction effect

Inactive Publication Date: 2020-05-29
佛山市鼎科科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the current grape seed extraction method, the extraction efficiency is low, and there are still some proanthocyanidins in the extraction residue, resulting in a certain waste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Extract grape seed extract from grape seeds.

[0023] A method for extracting grape seed extract with high proanthocyanidin content, comprising the steps of:

[0024] Step S10: Select fresh Muscat grapes, manually remove the skin and flesh of the grapes, and keep the grape seeds, then use a soft brush and clean water to remove the residues and other impurities on the surface of the grape seeds, and finally use a fan to clean the grape seeds. Air dry and blow away other impurities;

[0025] Step S20: Add absolute ethanol to the grape seeds obtained in step S10 until the grape seeds are completely submerged, grind the grape seeds for 4 minutes, pour the grape seeds and the liquid produced by grinding into an oil press for pressing and store the pressed The extracted grape oil, that is, the liquid squeezed out of the oil press, the squeezed grape seeds are taken out and rinsed and filtered with distilled water, the filtrate and grape oil are combined, and th...

Embodiment 2

[0032] Embodiment 2: Extraction efficiency test of the extraction method.

[0033] With the grape seed extract in embodiment one (the extract that obtains in step S60) as experimental group 1, make control group and other experimental groups according to the condition of table 1 again, detect the proanthocyanidins of the grape seed extract that each group obtains again content.

[0034] Table 1, the difference table of the processing parameters between the control group and the experimental group.

[0035] Experimental group 1 Experimental group 2 Experimental group 3 Control group 4 Control group 5 Ultrasonic Assisted Treatment + + - - - microwave assisted processing + - - - - Press treatment + + + - + Leaching treatment + + + + -

[0036] Detection method of proanthocyanidin content:

[0037] Accurately weigh a certain quality of proanthocyanidin standard substance, dissolve it with petroleum ether and set the ...

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Abstract

The invention provides an extraction method of a grape seed extract with high procyanidine content. Ethanol squeezing method is adopted fristly, after squeezing, digestion is carried out under the assistance of ultrasonic waves and microwaves; finally, a leach liquor and a grape oil are combined and purified, the extraction efficiency is improved through two times of extraction, meanwhile, the extraction capacity of the secondary extraction is enhanced through double-source ultrasonic treatment, the extraction efficiency is further improved, and the grape seed extract extraction method has theadvantage of being high in extraction efficiency.

Description

technical field [0001] The invention belongs to the technical field of plant component extraction, and in particular relates to a method for extracting grape seed extract with high proanthocyanidin content. Background technique [0002] Proanthocyanidins, referred to as PC, is a general term for a large class of polyphenolic compounds that widely exist in plants. Its oligomers: oligomeric proanthocyanidins (OPC) are internationally recognized as effective natural antioxidants for scavenging free radicals in the human body. Typically grape seed extract or French maritime pine bark extract. Proanthocyanidins (grape seed extract) is a new type of high-efficiency antioxidant with strong in vivo activity. Experiments have proved that the anti-free radical oxidation ability of oligomeric proanthocyanidins OPC is 50 times that of vitamin E and 20 times that of vitamin C, and it can be absorbed quickly and completely. It can reach the highest blood concentration in 20 minutes after...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 张璇余欢罗晓滢范燕雯
Owner 佛山市鼎科科技发展有限公司
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