Preparation method of diluent for storing sheep semen at normal temperature

A technology of room temperature storage and diluent, which is applied in the field of sheep breeding, can solve the problems of prolonging the storage time of semen and the decline of sperm motility, and achieve the effect of prolonging the total survival time, prolonging the effective survival time, and simple configuration process

Inactive Publication Date: 2020-06-05
YANGZHOU UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to address the above-mentioned existing problems, to provide a method for preparing a diluent of sheep semen stored at room temperature, to provide the application effect of yolk in the diluent formula of sheep semen stored at room temp

Method used

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  • Preparation method of diluent for storing sheep semen at normal temperature
  • Preparation method of diluent for storing sheep semen at normal temperature
  • Preparation method of diluent for storing sheep semen at normal temperature

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Embodiment Construction

[0022] The present invention will be further described below in combination with specific embodiments.

[0023] Materials and Methods;

[0024] 1. Test reagents and consumables

[0025] Tris (A100826-0500), fructose (A100226-0500), citric acid (A100529-0250) and 0.22 μm filter membrane (F513142-0001) were purchased from Sangon Bioengineering (Shanghai) Co., Ltd.; penicillin sodium (S17030-100g ) and streptomycin sulfate (S17058-100g) were purchased from Yuanye Biology (Shanghai) Technology Co., Ltd.; electronic analytical balance (AB204-N) was purchased from Mettler Toledo Measuring Equipment (Shanghai) Co., Ltd.

[0026] 2. Specific operation process;

[0027] Preparation of sheep semen storage diluent at room temperature: First, fully dissolve 30.2-31.2g Tris, 15.9-16.9g citric acid, 19.5-20.5g fructose, 0.3-0.4g penicillin sodium, 0.6-0.7g streptomycin sulfate in 1000mL Bacteria in ultrapure water, and then use a 0.22 μm filter membrane to perform filter sterilization. A...

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Abstract

The invention relates to a preparation method of a diluent for storing sheep semen at a normal temperature. The preparation method comprises the following steps: 1, dissolving 30.2 g to 31.2 g of Tris, 15.9 g to 16.9 g of citric acid, 19.5 g to 20.5 g of fructose, 0.3 g to 0.4 g of penicillin sodium and 0.6 g to 0.7 g of streptomycin sulfate into 1000mL of sterilized ultrapure water to obtain a basic diluent, 2, when 95 mL of the basic diluent is taken, adding 5 mL of yolk into 95 mL of the basic diluent so that a first diluent is obtained, when 85mL of the basic diluent is taken, adding 15mLof yolk into the 85mL of the basic diluent to obtain a second diluent, when 80 mL of the basic diluent is taken, adding 20 mL of yolk into 80 mL of the basic diluent so that a third diluent is obtained, putting the first diluent, the second diluent and the third diluent into a refrigerator to be fully dissolved, then centrifuging for 20 minutes at the speed of 10,000 r/min and the temperature of 4DEG C, and taking out a supernatant after centrifuging, so as to obtain a yolk diluent with the concentration of 5%, a yolk diluent with the concentration of 15% and a yolk diluent with the concentration of 20%. Through the diluent provided by the invention, the semen preservation effect is improved.

Description

technical field [0001] The invention relates to a method for preparing a diluent of sheep semen stored at room temperature, and belongs to the technical field of sheep reproduction. Background technique [0002] Artificial insemination technology is a basic technology in livestock breeding, which can make full use of the semen of good breeding males, and is an important means to improve the reproductive efficiency and productivity of livestock. Artificial insemination technology can reduce breeding costs, prevent the spread of diseases, and achieve large-scale breeding and management. Semen preservation is a key link in artificial insemination technology. Semen preservation is usually divided into normal temperature preservation, cryopreservation and cryopreservation. In actual production, semen is mostly stored at room temperature, which has the advantages of simple processing and operation, suitable for popularization and application, and no special temperature control a...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 李拥军张柳明冯云奎马金亮王健
Owner YANGZHOU UNIV
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