Preparation method of gelatin-collagen mixture and preparation method of hydrogel material
A mixture and collagen technology, applied in the field of medical biomaterials, can solve the problem of difficult mixing of collagen and gelatin, and achieve the effect of enhanced mechanical properties and adjustable viscoelastic properties
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[0053] Due to the different melting temperatures of gelatin and collagen, how to effectively mix collagen and gelatin and use the two to build composite materials has always been a difficult point in the industry. In order to solve the above problems, the inventor found through research that neutral collagen solution can be assembled at 30°C to obtain collagen hydrogel, while gelatin can be swelled in cold water and dissolved in hot water at 30°C. The invention proposes a method for effectively and uniformly mixing gelatin and collagen to prepare a gelatin-collagen mixture. see figure 1 As shown, in one embodiment of the preparation method of the gelatin-collagen mixture provided by the present invention, the preparation method of the gelatin-collagen mixture comprises the following steps:
[0054] Step S10, mixing collagen with weak acid at 2-10°C to prepare an acidic collagen solution;
[0055] There are many types of collagen, and the common types are type I, type II, typ...
Embodiment 1
[0076] The preparation of embodiment 1 gelatin-collagen mixture
[0077] (1) At a temperature of 5°C, mix natural type I collagen (bovine Achilles tendon collagen) with acetic acid with a molar concentration of 0.5 mol / L to prepare an acidic collagen solution with a collagen mass concentration of 2 mg / mL;
[0078] (2) At a temperature of 5° C., using a dialysis membrane with a molecular weight cut-off of 10,000, the acidic collagen solution is dialyzed with a phosphate buffer until a neutral collagen solution is obtained;
[0079] (3) Place the neutral collagen solution in a low-temperature centrifuge, and centrifuge at a speed of 8000r / min for 10 minutes at a temperature of 5°C to remove air bubbles in the neutral collagen solution. The rotor of the centrifuge is a fixed-angle rotor. The angle is 30°;
[0080] (4) Put the block gelatin into the grinding jar, put the grinding jar into liquid nitrogen and freeze for 20 minutes, then take out the grinding jar and put it into th...
Embodiment 2
[0082] The preparation of embodiment 2 gelatin-collagen mixture
[0083] (1) At a temperature of 2°C, mix natural type I collagen (bovine Achilles tendon collagen) with acetic acid with a molar concentration of 0.1 mol / L to prepare an acidic collagen solution with a collagen concentration of 1 mg / mL;
[0084] (2) At a temperature of 2°C, using a dialysis membrane with a molecular weight cut-off of 8000, the acidic collagen solution is dialyzed with a phosphate buffer until a neutral collagen solution is obtained;
[0085] (3) Place the neutral collagen solution in a low-temperature centrifuge, and centrifuge at a speed of 7000r / min for 12 minutes at a temperature of 2°C to remove air bubbles in the neutral collagen solution. The rotor of the centrifuge is a fixed-angle rotor. The angle is 45°;
[0086] (4) Put the block gelatin into the grinding jar, put the grinding jar into liquid nitrogen and freeze for 15 minutes, then take out the grinding jar and put it into the grinder...
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