Preparation method of gelatin-collagen mixture and preparation method of hydrogel material

A mixture and collagen technology, applied in the field of medical biomaterials, can solve the problem of difficult mixing of collagen and gelatin, and achieve the effect of enhanced mechanical properties and adjustable viscoelastic properties

Pending Publication Date: 2020-06-12
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The main purpose of the present invention is to propose a preparation method of gelatin-collagen mixture and a preparation method of hydrogel material, aiming at solving the problem that it is difficult to effectively mix collagen and gelatin

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  • Preparation method of gelatin-collagen mixture and preparation method of hydrogel material
  • Preparation method of gelatin-collagen mixture and preparation method of hydrogel material
  • Preparation method of gelatin-collagen mixture and preparation method of hydrogel material

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preparation example Construction

[0053] Due to the different melting temperatures of gelatin and collagen, how to effectively mix collagen and gelatin and use the two to build composite materials has always been a difficult point in the industry. In order to solve the above problems, the inventor found through research that neutral collagen solution can be assembled at 30°C to obtain collagen hydrogel, while gelatin can be swelled in cold water and dissolved in hot water at 30°C. The invention proposes a method for effectively and uniformly mixing gelatin and collagen to prepare a gelatin-collagen mixture. see figure 1 As shown, in one embodiment of the preparation method of the gelatin-collagen mixture provided by the present invention, the preparation method of the gelatin-collagen mixture comprises the following steps:

[0054] Step S10, mixing collagen with weak acid at 2-10°C to prepare an acidic collagen solution;

[0055] There are many types of collagen, and the common types are type I, type II, typ...

Embodiment 1

[0076] The preparation of embodiment 1 gelatin-collagen mixture

[0077] (1) At a temperature of 5°C, mix natural type I collagen (bovine Achilles tendon collagen) with acetic acid with a molar concentration of 0.5 mol / L to prepare an acidic collagen solution with a collagen mass concentration of 2 mg / mL;

[0078] (2) At a temperature of 5° C., using a dialysis membrane with a molecular weight cut-off of 10,000, the acidic collagen solution is dialyzed with a phosphate buffer until a neutral collagen solution is obtained;

[0079] (3) Place the neutral collagen solution in a low-temperature centrifuge, and centrifuge at a speed of 8000r / min for 10 minutes at a temperature of 5°C to remove air bubbles in the neutral collagen solution. The rotor of the centrifuge is a fixed-angle rotor. The angle is 30°;

[0080] (4) Put the block gelatin into the grinding jar, put the grinding jar into liquid nitrogen and freeze for 20 minutes, then take out the grinding jar and put it into th...

Embodiment 2

[0082] The preparation of embodiment 2 gelatin-collagen mixture

[0083] (1) At a temperature of 2°C, mix natural type I collagen (bovine Achilles tendon collagen) with acetic acid with a molar concentration of 0.1 mol / L to prepare an acidic collagen solution with a collagen concentration of 1 mg / mL;

[0084] (2) At a temperature of 2°C, using a dialysis membrane with a molecular weight cut-off of 8000, the acidic collagen solution is dialyzed with a phosphate buffer until a neutral collagen solution is obtained;

[0085] (3) Place the neutral collagen solution in a low-temperature centrifuge, and centrifuge at a speed of 7000r / min for 12 minutes at a temperature of 2°C to remove air bubbles in the neutral collagen solution. The rotor of the centrifuge is a fixed-angle rotor. The angle is 45°;

[0086] (4) Put the block gelatin into the grinding jar, put the grinding jar into liquid nitrogen and freeze for 15 minutes, then take out the grinding jar and put it into the grinder...

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Abstract

The invention discloses a preparation method of a gelatin-collagen mixture and a preparation method of a hydrogel material. The preparation method of the gelatin-collagen mixture comprises the following steps: mixing collagen with a weak acid at 2-10 DEG C to prepare an acidic collagen solution; adjusting the acidic collagen solution to be neutral at 2-10 DEG C to obtain a neutral collagen solution; carrying out pre-freezing treatment on gelatin, and then grinding the gelatin into powder to prepare gelatin powder; and mixing the gelatin powder with the neutral collagen solution at 2-10 DEG C to prepare the gelatin-collagen mixture. The cold water swelling property of gelatin is utilized, the gelatin powder is dispersed into the neutral collagen solution, and physical blending is carried out in a low-temperature environment to prepare the uniform gelatin-collagen mixture, so uniform mixing of gelatin and a neutral collagen raw material is realized for the first time, the method is simple, convenient and efficient, and the gelatin-collagen mixture prepared by the method has self-assembly performance and can be used for preparing the hydrogel material through self-assembly.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to a method for preparing a gelatin-collagen mixture and a method for preparing a hydrogel material. Background technique [0002] Collagen, also known as collagen, is a white opaque, unbranched and insoluble fibrous protein, which is a very important natural polymer resource. It has a wide range of sources and mainly exists in animal skin, cartilage, bone, ligaments and blood vessels. It plays an important role in supporting, protecting, combining and forming interface partitions. Because collagen has good biocompatibility, low antigenicity, degradability, ability to promote cell proliferation, hemostatic performance, moisture retention, anti-aging and other properties, it has broad application prospects in various industries, but simple Collagen has disadvantages such as poor solubility and poor mechanical properties. [0003] Gelatin is obtained by the hydrolysis ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/00C08J3/075C08L89/00
CPCC08J3/005C08J3/075C08J2389/00C08J2489/00
Inventor 何浪汪海波未本美吴溪林熊志进黄荣瑞朱北荣曹杨
Owner WUHAN POLYTECHNIC UNIVERSITY
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