Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Horseradish flower bolt callus culture method capable of effectively inhibiting browning

A technology of callus and culture method, which is applied in the field of culture of wasabi flower callus, and can solve the problems of death, browning of culture materials, etc.

Active Publication Date: 2020-06-16
CHENGDU UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the wasabi flower moss cells contain more isothiocyanate compounds and other easily oxidized substances, severe browning often occurs during the callus induction and mass proliferation culture process, resulting in a large number of death of culture materials. This is also a major problem in the current large-scale production of wasabi active ingredients using biotechnology
There are no reports of related solutions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A kind of method for cultivating the wasabi flower callus that can effectively inhibit the generation of browning provided by the present embodiment comprises the following steps:

[0017] (1) Selection and disinfection of explants: select the flower moss with a length of 15 cm under the terminal bud of wasabi flower moss as an explant, put it on the ultra-clean workbench, and sterilize it with 0.1% mercuric chloride for 6 minutes. Rinse 5 times with cefoperazone sulbactam sodium sterile water with a concentration of 60mg / L, then blot the moisture on the surface with sterile filter paper;

[0018] (2) Callus induction culture: cut the sterilized horseradish moss into 1.5cm length, and then quickly insert the improved MS+6-BA1.0mg / L+2,4-D3.0mg / L+tyrosine In the callus induction medium of 75g / L+glucose 15g / L+sorbose 10g / L+agar 5.0g / L, cultivate under the condition that the culture temperature is 20°C, the light is 6h every day and the light intensity is 400lx. The iron s...

Embodiment 2

[0023] A kind of method for cultivating the wasabi flower callus that can effectively inhibit the generation of browning provided by the present embodiment comprises the following steps:

[0024] (1) Selection and disinfection of explants: select the flower moss with a length of 10 cm under the terminal bud of wasabi flower moss as an explant, put it on the ultra-clean workbench, and sterilize it with 0.1% mercuric chloride for 4 minutes. Rinse 3 times with cefoperazone sulbactam sodium sterile water with a concentration of 40 mg / L, then blot the surface moisture with sterile filter paper;

[0025] (2) Callus induction culture: cut the sterilized horseradish moss into 1cm length, and then quickly insert the improved MS+6-BA0.5mg / L+2,4-D2.0mg / L+tyrosine 50g In the callus induction medium of / L+glucose 10g / L+sorbose 5g / L+agar 4.5g / L, the culture was carried out under the condition that the culture temperature was 18°C, the light was 4h per day, and the light intensity was 300lx....

Embodiment 3

[0030] A kind of method for cultivating the wasabi flower callus that can effectively inhibit the generation of browning provided by the present embodiment comprises the following steps:

[0031] (1) Selection and disinfection of explants: select the flower moss with a length of 20 cm under the terminal bud of wasabi flower moss as an explant, put it on the ultra-clean workbench, and sterilize it with 0.1% mercuric chloride for 8 minutes. Rinse 6 times with cefoperazone sulbactam sodium sterile water with a concentration of 80mg / L, then blot the moisture on the surface with sterile filter paper;

[0032] (2) Callus induction culture: cut the sterilized horseradish moss into 2cm length, and then quickly insert the improved MS+6-BA1.5mg / L+2,4-D4.0mg / L+tyrosine 100g In the callus induction medium of / L+glucose 20g / L+sorbose 15g / L+agar 5.5g / L, culture is carried out under the condition of culture temperature of 22°C, light of 8h per day and light intensity of 500lx, the improved M...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a horseradish flower bolt callus culture method capable of effectively inhibiting browning. According to the method, a material flushed and disinfected through sterile water containing Cefoperazone and Sulbactam is innovatively utilized aiming at situations that horseradish flower bolt explants usually seriously brown during induction of calluses and vast enrichment cultureso as to cause death of a large number of cultures, the induction of the calluses and constituents of an enrichment culture medium are improved, and meanwhile, a step for carrying out preprocessing by virtue of a water solution containing cefotaxime sodium is increased before the enrichment culture of the calluses of horseradishes. According to the method, the problem that the horseradish flowerbolt explants usually seriously brown during the induction of the calluses and the enrichment culture is effectively solved, and a new way is provided for production of effective components of horseradish plants; and meanwhile, a certain theoretical foundation is provided for deeper execution of biotechnology researches of the horseradish plants.

Description

technical field [0001] The invention relates to a method for cultivating wasabi callus, in particular to a method for cultivating wasabi callus capable of effectively inhibiting browning. Background technique [0002] Wasabi japonica (Wasabi japonica Matsum) is a perennial semi-shade herbaceous plant belonging to the Brassicaceae family. [0003] The whole plant of wasabi contains a kind of myrosinin substance. When the plant cells are ground, the substance is hydrolyzed under the action of myrosinase to form isothiocyanate compounds, which have the functions of sterilization, antiseptic, diuresis and blood cleansing. , anti-infection, anti-cancer and other medicinal activities, this product is favored by more and more people, and is internationally recognized as an extremely rare and precious medicinal and edible homologous plant, known as "green gold". However, because the growth and development of wasabi plants are extremely demanding on the environment, it is only suita...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/001
Inventor 王跃华吴桐宇吴嘉琪陈芳马涛刘真珍刘崧任魏博坤何佳懋许班高健
Owner CHENGDU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com