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A culture medium, Euglena technology, applied in cell culture medium, culture process, tissue culture, etc., can solve the problems of algal cell rupture, reduction of pre-concentration efficiency, algal cell precipitation, etc.
Active Publication Date: 2020-06-26
YOUGE TIANCHENG BIOTECH YIWU CO LTD
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At present, the flocculation method is more economical, but the traditional algae cell culture medium can only adjust the pH to above 10.5 to cause algal cell flocculation, and then algal cell precipitation to achieve the purpose of pre-concentration. Cells rupture due to excessive osmotic pressure, or destroy the active substances of algae cells, which reduces the efficiency of pre-concentration; and excessive pH affects sewage discharge standards and requires more acid feed to restore the original pH value, thereby increasing processing cost
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Embodiment 1
[0049] A kind of euglena culture medium, comprises the component of following concentration: 1.8g / L NH 4 Cl, 0.6g / L KH 2 PO 4 , 1.2g / LMgSO 4 ·7H 2 O, 0.02g / L CaCl 2 2H 2 O, 0.55 μg / L Na 2 EDTA·2H 2 O, 2μg / L Fe 2 (SO 4 ) 3 , 0.05μg / LCuSO 4 ·5H 2 O, 0.4 μg / L ZnSO 4 ·7H 2 O, 1.3μg / L Co(NH 3 )·H 2 O, 1.8 μg / L MnCl2 4H 2 O, 0.01μg / L Vitamin B 1 and 0.0005μg / L Vitamin B 12 .
[0050] Example 1 The main ion composition table of the euglena culture medium is shown in Table 1, and the main metal ion is Mg 2+ and Ca 2+ .
[0051] Table 1 The main ionic components of euglena medium
[0052]
Embodiment 2
[0054] A kind of euglena culture medium, comprises the component of following concentration: 1.5g / L NH 4 Cl, 0.6g / L KH 2 PO 4 , 1.2g / LMgSO 4 ·7H 2 O, 0.02g / L CaCl 2 2H 2 O, 0.55 μg / L Na 2 EDTA·2H 2 O, 2μg / L Fe 2 (SO 4 ) 3 , 0.05μg / LCuSO 4 ·5H 2 O, 0.4 μg / L ZnSO 4 ·7H 2 O, 1.2μg / L Co(NH 3 )·H 2 O, 1.8 μg / L MnCl 2 4H 2 O, 0.01 μg / L vitamin B1 and 0.0005 μg / L vitamin B12.
Embodiment 3
[0056] A euglena medium comprising components at the following concentrations: 2.7g / L NH 4 Cl, 2.4g / L KH 2 PO 4 , 2.4g / LMgSO 4 ·7H 2 O, 0.10g / L CaCl 2 2H 2 O, 0.78 μg / L Na 2 EDTA·2H 2 O, 4μg / L Fe 2 (SO 4 ) 3 , 0.08μg / LCuSO 4 ·5H 2 O, 0.7μg / L ZnSO 4 ·7H 2 O, 1.5μg / L Co(NH 3 )·H 2 O, 2.2 μg / L MnCl 2 4H 2 O, 0.09 μg / L vitamin B1 and 0.0015 μg / L vitamin B12.
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Abstract
The invention belongs to the technical field of biology, and particularly relates to a euglena culture medium and an application thereof. The euglena culture medium provided by the invention comprisesthe following components: NH4Cl, KH2PO4, MgSO4.7H2O, CaCl2.2H2O, Na2EDTA.2H2O, Fe2(SO4)3, CuSO4.5H2O, ZnSO4.7H2O, Co(NH3).H2O, MnCl2.4H2O, vitamin B1 and vitamin B12. The pH value is adjusted on thebasis of the euglena culture medium provided by the invention, and the aim of pre-concentrating euglena cells is achieved. According to the invention, efficiency of concentrating euglena cells can beimproved, rupture of the euglena cells is less, and thus harvesting cost can be reduced.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an euglena culture medium and an application thereof. Background technique [0002] As a single-cell, cell-wall-free microalgae with flagella, Euglena is widely used in carbon emission reduction, food additives, natural pigments, unsaturated fatty acids, and biofuels. However, the diameter of the cells of Euglena is about Under autotrophic conditions, the biomass is only within the range of 0.5-2g / L. Therefore, it is difficult to harvest the algal cells. If the algal cells are directly concentrated by a centrifuge, the production cost will be increased. Therefore, a method for pre-concentrating the euglena cells is currently needed to reduce the production cost. [0003] At present, the methods for pre-concentrating microalgae include electrolysis, air flotation, gravity precipitation, ultrafiltration membrane filtration and flocculation. At present, the flocculation me...
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