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Methods of modulating alk

A technology of reagents and polynucleotides, applied in biochemical equipment and methods, pharmaceutical formulations, recombinant DNA technology, etc.

Pending Publication Date: 2020-07-03
SOC DES PROD NESTLE SA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] While various methods are known to promote weight loss, subjects are at risk of regaining lost weight once they complete the weight loss intervention period

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0202] Example 1: Association between ALK gene variants and lean phenotype

[0203] group selection : This study involves a genetic association study in constitutionally thin (CT) individuals using data from the EGCUT biobank. Estonia, through the Estonian Genomics Center (EGCUT) at the University of Tartu, has established a biobank based on biological samples (DNA, RNA and plasma) from the general population and clinical data from 60,000 individuals. The lean phenotype was defined as those in the lowest 6% of age- and sex-adjusted BMI, and strict exclusion criteria included: pregnancy, postmenopausal or menstrual abnormalities, vegetarians, or known food intolerances (allergies) Subjects, according to DSMIV Subjects with eating disorders, drinking more than 10 drinks per week, suffering from serious chronic diseases (such as diabetes), excessive physical activity (more than 3 training sessions per week), smoking more than 10 drinks per week depression or psychiatric illn...

Embodiment 2

[0221] Example 2: In vivo function of ALK

[0222] fly race : Fly stocks were maintained on a standard diet with agar, sugar and yeast and were reared in a 25°C incubator on a 12 / 12 dark and night cycle. Actin-Gal4 from Bloomington, and w1118 and UAS-Alk IR (GD11446) from VDRC.

[0223] Triglyceride determination : 10 (4-7 days old) male flies were weighed 5 times and incubated on ice in 200 μl dHO 2 Homogenize in O, then sonicate for 10 s on ice using a probe sonicator. After sonication, add 800 μl of ice-cold dHO 2 O and mix well. 50 [mu]l of the mixture was used for the determination of triglycerides using the Roche triglyceride kit (11730711216) according to the manufacturer's instructions. Body weight was measured by analytical balance. Triglycerides were normalized to body weight.

[0224] result : To study ALK gene function in vivo, we used transgenic RNAi in Drosophila (Drosophila melanogaster). ALK shows high homology to the fly Alk gene. Using system...

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Abstract

An agent capable of decreasing the activity of ALK for use in supporting weight maintenance and / or treating or preventing obesity.

Description

technical field [0001] The present invention relates to agents capable of modulating the activity of ALK and the use of such agents in therapy, especially in supporting weight maintenance and treating obesity. The invention also relates to methods of identifying such agents. Background technique [0002] Obesity is a chronic metabolic disease that has reached epidemic proportions in many parts of the world. Obesity is a major risk factor for serious comorbidities such as type 2 diabetes, cardiovascular disease, dyslipidemia and certain types of cancer (World Health Organ. Tech. Rep. Ser. (2000) 894: i-xii, 1-253 (World Health Organization Technical Report Series, 2000, 894:i-xii, pp. 1-253)). [0003] Obesity refers to a condition in which an individual's body weight exceeds normal due to excessive accumulation of energy from carbohydrates, fats, etc. Extra body weight is usually retained under the skin or around internal organs in the form of fat. Empirical data show th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/573C12N15/113A61P3/04
CPCA61K31/573C12N15/1135A61P3/04C12N2310/11C12N2310/14C12N2310/141C12Q1/6876G01N33/5023G01N2333/912
Inventor N·格尔多夫A·瓦尔塞西亚J·哈格尔
Owner SOC DES PROD NESTLE SA