Golden clematis seedling breeding medium and preparation method thereof, and golden clematis seedling breeding method
A technology of culture medium and induction medium, which is applied in the direction of botany equipment and methods, gardening methods, gardening tools/equipment, etc., can solve the problem of high cost, achieve good supporting effect, shorten the cultivation time, and achieve the effect of excellent cultivation effect
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[0027] The present invention also provides a preparation method based on the medium described in the above technical scheme, comprising the following steps:
[0028] The raw materials were mixed, stirred at 100° C. for more than 10 minutes, and sterilized at high temperature and high pressure to obtain the culture medium. In the present invention, the stirring time is 10-25 minutes, more preferably 10 minutes. In the present invention, the high temperature and high pressure sterilization conditions are 121°C, 204.9kPa, 20min. In the present invention, the agitation is preferably sufficient agitation. In the present invention, the setting of the agitation conditions can realize the mixing of each component in the medium, fully gelatinize the medium, and ensure that the components of the medium are uniform. , in a transparent, solidified state.
[0029] The present invention also provides a method for breeding clematis seedlings based on the medium described in the above techn...
Embodiment 1
[0037] Set up 6 temperature gradients (20°C, 40°C, 60°C, 80°C, 90°C, 100°C), and set 3 repetitions for each gradient, using MS medium as the basal medium, adding NAA3mg / L, 6-BA1 .5 mg / L, sucrose 20 / L, starch 50g / L, keep the water bath at constant temperature for 20min under the above-mentioned different temperature conditions, fully stir, and then divide the culture medium solution into tissue culture bottles (about 50ml per bottle). Sterilize under high temperature and high pressure, and observe the solidification after cooling.
[0038] After thorough stirring, the aqueous medium solution gradually changed from pure white to light yellow. After cooling in a constant temperature water bath for 20 minutes, the pH values of the culture medium solutions at five temperature gradients were all between 6.9 and 7.1. It can be seen from observation that the aqueous starch solution heated to 80°C is white and semi-solidified, and the aqueous starch solution heated to 100°C is trans...
Embodiment 2
[0043] Under different time gradients (5min, 10min, 15min, 20min, 25min), each gradient was repeated three times, and the composition of the medium was the same as in Example 1. The medium solution was boiled and fully stirred, and then heated at 121°C for 20min. After autoclaving, observe the solidification after cooling.
[0044] After cooling, the pH of the culture medium was measured to be between 6.9 and 7.1. Except that the 3 repetitions of boiling for 5 minutes were in a semi-solidified state, all other treatments were solidified. The setting of 10 minutes of boiling time in the present invention can ensure that the culture medium is in a good solidification state. The results of the coagulation state of the medium under different boiling times are shown in Table 2.
[0045] Table 2 The coagulation state of the medium under different boiling times
[0046]
[0047]
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