Golden clematis seedling breeding medium and preparation method thereof, and golden clematis seedling breeding method

A technology of culture medium and induction medium, which is applied in the direction of botany equipment and methods, gardening methods, gardening tools/equipment, etc., can solve the problem of high cost, achieve good supporting effect, shorten the cultivation time, and achieve the effect of excellent cultivation effect

Active Publication Date: 2021-02-19
浙江省中药研究所有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cultivation of clematis at present still has the defect that the cost is relatively high

Method used

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  • Golden clematis seedling breeding medium and preparation method thereof, and golden clematis seedling breeding method
  • Golden clematis seedling breeding medium and preparation method thereof, and golden clematis seedling breeding method
  • Golden clematis seedling breeding medium and preparation method thereof, and golden clematis seedling breeding method

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0027] The present invention also provides a preparation method based on the medium described in the above technical scheme, comprising the following steps:

[0028] The raw materials were mixed, stirred at 100° C. for more than 10 minutes, and sterilized at high temperature and high pressure to obtain the culture medium. In the present invention, the stirring time is 10-25 minutes, more preferably 10 minutes. In the present invention, the high temperature and high pressure sterilization conditions are 121°C, 204.9kPa, 20min. In the present invention, the agitation is preferably sufficient agitation. In the present invention, the setting of the agitation conditions can realize the mixing of each component in the medium, fully gelatinize the medium, and ensure that the components of the medium are uniform. , in a transparent, solidified state.

[0029] The present invention also provides a method for breeding clematis seedlings based on the medium described in the above techn...

Embodiment 1

[0037] Set up 6 temperature gradients (20°C, 40°C, 60°C, 80°C, 90°C, 100°C), and set 3 repetitions for each gradient, using MS medium as the basal medium, adding NAA3mg / L, 6-BA1 .5 mg / L, sucrose 20 / L, starch 50g / L, keep the water bath at constant temperature for 20min under the above-mentioned different temperature conditions, fully stir, and then divide the culture medium solution into tissue culture bottles (about 50ml per bottle). Sterilize under high temperature and high pressure, and observe the solidification after cooling.

[0038] After thorough stirring, the aqueous medium solution gradually changed from pure white to light yellow. After cooling in a constant temperature water bath for 20 minutes, the pH values ​​of the culture medium solutions at five temperature gradients were all between 6.9 and 7.1. It can be seen from observation that the aqueous starch solution heated to 80°C is white and semi-solidified, and the aqueous starch solution heated to 100°C is trans...

Embodiment 2

[0043] Under different time gradients (5min, 10min, 15min, 20min, 25min), each gradient was repeated three times, and the composition of the medium was the same as in Example 1. The medium solution was boiled and fully stirred, and then heated at 121°C for 20min. After autoclaving, observe the solidification after cooling.

[0044] After cooling, the pH of the culture medium was measured to be between 6.9 and 7.1. Except that the 3 repetitions of boiling for 5 minutes were in a semi-solidified state, all other treatments were solidified. The setting of 10 minutes of boiling time in the present invention can ensure that the culture medium is in a good solidification state. The results of the coagulation state of the medium under different boiling times are shown in Table 2.

[0045] Table 2 The coagulation state of the medium under different boiling times

[0046]

[0047]

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Abstract

The present invention relates to an anoectochilus formosanus seedling propagation culture medium, a preparation method of the culture medium and an anoectochilus formosanus seedling propagation method, and belongs to the technical field of anoectochilus formosanus propagation. In the culture medium disclosed by the invention, an anoectochilus formosanus seedling induction culture medium takes MS as a basic culture medium, and further comprises 2.0-4.5 mg / L of NAA, 0.5-3.0 mg / L of 6-BA, 20-40 g / L of sucrose, 1-4 g / L of activated carbon and 32-50 g / L of starch; an anoectochilus formosanus strongseedling culture medium takes 1 / 2 MS as the basic culture medium, and further comprises 0.5-4.0 mg / L of the NAA, 20-40 g / L of the sucrose, 1-4 g / L of the activated carbon and 32-50 g / L of the starch.The culture medium disclosed by the invention not only can provide rich nutrient substances, but also can take the good supporting effect for anoectochilus formosanus, thereby shortening the culturetime, improving the seedling quality and reducing the production cost.

Description

technical field [0001] The invention relates to the technical field of clematis breeding, in particular to a clematis seedling breeding medium, a preparation method thereof, and a clematis seedling breeding method. Background technique [0002] Golden clematis, orchidaceae, genus Sclerophyllum, rhizomes are prostrate, fleshy, with nodes, and root on the nodes. Clematis is born in evergreen broad-leaved forests or damp places in valleys at an altitude of 50-1600 meters. Produced in Zhejiang, Jiangxi, Fujian and other provinces and regions in China. Auranthus clematis tastes sweet and flat, has the effects of clearing heat and cooling blood, detoxifying and reducing swelling, moistening lung and relieving cough, used for hemoptysis, cough, phlegm and dyspnea, astringent pain in urination, thirst, chyluria, convulsions in children, aphtha, heart disease, etc. Diseases, poisonous snake bites also have definite curative effect. The cultivation of clematis still has the defect ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008
Inventor 沈宇峰沈晓霞孙岚孙健
Owner 浙江省中药研究所有限公司
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