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Application of LINC01419 as marker for diagnosing and treating hepatocellular carcinoma

A technology for hepatocellular carcinoma and drugs, applied in the field of long non-coding RNA, which can solve problems such as increasing the risk of HCC

Inactive Publication Date: 2020-08-04
湖南省科域生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

GSTP1 gene mutations in AG and GG genotypes increase the risk of HCC

Method used

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  • Application of LINC01419 as marker for diagnosing and treating hepatocellular carcinoma
  • Application of LINC01419 as marker for diagnosing and treating hepatocellular carcinoma
  • Application of LINC01419 as marker for diagnosing and treating hepatocellular carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1: lncRNA LINC01419 is a long non-coding RNA involved in the pathological process of HCC

[0066]Through the analysis of HCC-related microarray expression profile data (GSE40367 and GSE45267), it was found that lncRNA LINC01419 was the most significantly up-regulated long non-coding RNA ( Figure 2A and Figure 2B ); TCGA database further confirmed that LINC01419 was highly expressed in HCC and correlated with prognosis ( Figure 2C and Figure 2D ); Therefore, LINC01419 was selected as the long non-coding RNA in this study.

[0067] The lncATLAS website predicts that lncRNA LINC01419 is located in the nucleus ( Figure 2E ), through qRT-PCR detection, it was found that the mRNA of GAPDH, which is mainly located in the cytoplasm, basically exists in the cytoplasmic fraction, and the U6, which mainly exists in the nucleus, basically exists in the nuclear fraction, indicating that the nucleus and cytoplasm have been fully separated. The detection results of lo...

Embodiment 2

[0068] Example 2: LINC01419 is highly expressed in hepatocellular carcinoma

[0069] qRT-PCR detected the expression levels of lncRNA LINC01419 in HCC tissues and adjacent tissues respectively, and found that the expression level of lncRNA LINC01419 in HCC tissues was significantly higher than that in adjacent tissues (P Figure 3A .

[0070] qRT-PCR detected the expression level of lncRNALINC01419 in normal human hepatocytes and SMMC7721 cells respectively, and found that the expression level of lncRNALINC01419 in HCC (SMMC7721) cells was significantly higher than that in normal human hepatocytes (P Figure 3B ); in addition, from Figure 3B It can be seen that the LINC01419-shRNA group can significantly down-regulate the expression of lncRNA LINC01419. Among them, Blank group (without any treatment), LINC01419-cDNA group (transfected with LINC01419-cDNA lentivirus), LINC01419-shRNA group (transfected with LINC01419-shRNA lentivirus), NC group (Negative Control, NC) (transfecte...

Embodiment 3

[0071] Embodiment 3: Tumor formation experiment in nude mice

[0072] During the experiment, none of the mice died unexpectedly. The tumors of mice with subcutaneous tumors grew rapidly, and thick red tumor blood vessels were visible on the surface of the tumors. When the tumor mass was separated, it was found that the tumor tissue was fish-like, brittle and easy to bleed. Necrotic tissue can be seen, and the tumor and deep tissue are infiltrated and grown; the mice with orthotopic tumors failed after 42 days, and after the nude mice were sacrificed and the abdominal cavity was opened, it was found that there were uneven nodular tumor masses with rich blood vessels on the surface of the liver, and the tumor invaded most of the The liver, and invaded the lungs, mesentery and other parts, the macroscopic examination showed that the lungs were dark red, and the microscopic examination showed that the metastases were distributed in the surrounding lungs, mainly micrometastases in t...

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Abstract

The invention discloses a long-chain non-coding RNA marker for diagnosing and treating hepatocellular carcinoma. Specifically, the long-chain non-coding RNA marker is LINC01419. The invention discloses an application of LINC01419 in preparation of a product for diagnosing hepatocellular carcinoma and a pharmaceutical composition for treating hepatocellular carcinoma. The invention also discloses akit for diagnosing hepatocellular carcinoma and a pharmaceutical composition for treating hepatocellular carcinoma. The kit provided by the invention can be used as one of the means for early diagnosis of hepatocellular carcinoma. The pharmaceutical composition disclosed by the invention has a relatively good inhibition effect on hepatocellular carcinoma, and has important reference and practicalsignificance in treatment of hepatocellular carcinoma.

Description

technical field [0001] The present invention relates to a long-chain non-coding RNA related to hepatocellular carcinoma and application thereof, wherein the long-chain non-coding RNA is LINC01419. The invention belongs to the field of biomedicine, specifically to the technical field of molecular biology. Background technique [0002] Primary liver cancer (primary carcinoma of liver), referred to as liver cancer, is a malignant tumor that occurs in liver cells or intrahepatic bile duct epithelial cells, and it is also one of the most common malignant tumors in my country. In my country, more than 100,000 people die from liver cancer every year, and the number is increasing year by year. The diagnosis and treatment of liver cancer have become the focus of public health and clinical attention in our country. At present, researchers have not yet made a clear conclusion on the etiology and pathogenesis of liver cancer. The traditional concept is that hepatitis virus, aflatoxin,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11A61K45/00A61K31/7088A61P1/16A61P35/00A61P35/04
CPCA61K31/7088A61K45/00A61P1/16A61P35/00A61P35/04C12Q1/6886C12Q2600/136C12Q2600/158C12Q2600/178
Inventor 李丽丽刘佳利黄娅
Owner 湖南省科域生物医药科技有限公司
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