Procalcitonin and C-reactive protein combined detection kit

A technology of reacting protein and procalcitonin, which is applied in the biological field, can solve problems such as short reaction time, incomplete reaction, and poor precision, and achieve the effect of saving materials and improving precision

Active Publication Date: 2020-08-14
巴迪泰(广西)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] When the sample to be tested flows through the binding pad, the antigen in the sample is complexed with the coated fluorescent-labeled PCT antibody complex, biotin-labeled PCT antibody complex, fluorescent-labeled CRP antibody complex and quality control The reaction time of the substance is short and the reaction may be incomplete, resulting in poor precision; and the optimal storage condition for the reactio...

Method used

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  • Procalcitonin and C-reactive protein combined detection kit
  • Procalcitonin and C-reactive protein combined detection kit
  • Procalcitonin and C-reactive protein combined detection kit

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Experimental program
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Effect test

Embodiment 1

[0051] A method for preparing procalcitonin and C-reactive protein joint detection kit reagent strips, comprising the following steps:

[0052] S1. Screening antibodies and antigens: Purchase CRP detection antibodies, CRP antigens, streptavidin and chicken IgY and perform pairing experiments on them to screen out CRP detection antibodies, CRP antigens, and streptavidin with high specificity and good affinity Chicken IgY and chicken IgY, adjust CRP detection antibody, streptavidin, chicken IgY and CRP antigen to 0.01-5mg / mL, 1-10mg / mL, 0.01-5mg / mL, 0.01-5mg respectively with coating diluent / mL;

[0053] S2. Coating with antibodies and antigens: Prepare the nitrocellulose membrane 5 and correctly place it on the film sticking equipment, and stick the nitrocellulose membrane 5 on the pad card 4 through the film sticking equipment. Spray the chicken IgY adjusted with the coating diluent on the nitrocellulose membrane 5 with a spraying equipment to form a control line, and spray ...

Embodiment 2

[0056] A combined detection kit for procalcitonin and C-reactive protein, the pH of reaction buffer 1 is 7.4, and the components are: 10mM PBS, 200mM NaCl, 0.01% NaN 3 , 0.1% Tween 20, 0.01% IGEPAL CA-630, 1ug / mL fluorescently labeled CRP antibody complex, 0.03ug / mL fluorescently labeled Anti-chicken IgY antibody complex; The first liquid in 7.4 was prepared by freeze-drying. The components of the first liquid were: 10mM PBS, 0.05% BSA, 0.05% Sucrose, 0.01ug / mL biotin-labeled PCT antibody complex, 0.01ug / mL fluorescently-labeled Anti-chickenIgY antibody complex; the second probe 3 is obtained by freeze-drying the second liquid with a pH of 7.4, and the components of the second liquid are: 10mM PBS, 0.05% BSA, 0.05% Sucrose, 2ug / mL fluorescence The labeled PCT antibody complex; the reagent strip includes a pad card 4, a nitrocellulose membrane 5 laid on the pad card 4, a sample pad 6 sequentially arranged on the nitrocellulose membrane 5, a quality control line 7, a A detectio...

Embodiment 3

[0058] A combined detection kit for procalcitonin and C-reactive protein, the pH of reaction buffer 1 is 7.4, and the components are: 10mM PBS, 400mM NaCl, 0.25% NaN 3 , 1.5% Tween 20, 0.25% IGEPAL CA-630, 5ug / mL fluorescently labeled CRP antibody complex, 1.5ug / mL fluorescently labeled Anti-chicken IgY antibody complex; The first liquid in 7.4 was prepared by freeze-drying. The components of the first liquid were: 10mM PBS, 5% BSA, 2.5% Sucrose, 5ug / mL biotin-labeled PCT antibody complex, 5ug / mL fluorescently-labeled Anti- chicken IgY antibody complex; the second probe 3 is obtained by freeze-drying the second liquid with a pH of 7.4, and the components of the second liquid are: 10mMPBS, 5% BSA, 2.5% Sucrose, 11ug / mL fluorescently labeled PCT antibody complex; the reagent strip includes a pad card 4, a nitrocellulose membrane 5 laid on the pad card 4, a sample pad 6 sequentially arranged on the nitrocellulose membrane 5, a quality control line 7, and a first detection Line 8...

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Abstract

The invention discloses a procalcitonin and C-reactive protein combined detection kit. The kit comprises a kit body, a refrigeration mechanism, a control system, a reagent strip, a first detection object, a second detection object and a reaction buffer solution, the box body is divided into a first box body, a second box body and a third box body which are sequentially arranged in the length direction, the reagent strip, the first detection object and the second detection object are all stored in the first box body, the reaction buffer solution is stored in the third box body, the first detection object contains a biotin labeled PCT antibody complex and a fluorescently labeled Anti-chicken IgY antibody complex, the second detection object contains a fluorescently-labeled PCT antibody complex, and the reaction buffer solution contains a fluorescently-labeled CRP antibody complex and the fluorescently-labeled Anti-chicken IgY antibody complex. The precision of simultaneous detection of PCT and CRP is improved, and the stability of the detection reagent product is improved.

Description

technical field [0001] The present invention relates to the field of biotechnology. More specifically, the present invention relates to a combined detection kit for procalcitonin and C-reactive protein. Background technique [0002] The combined detection of procalcitonin and C-reactive protein (PCT / CRP) has certain clinical value for the comprehensive judgment of inflammation. [0003] The existing procalcitonin and C-reactive protein combined detection kit includes a box body, a reaction buffer arranged in the box body, and a reagent strip, wherein the reagent strip includes a pad card, a nitrocellulose membrane laid on the pad card, The sample pad, conjugation pad, quality control line, first detection line, second detection line and absorption pad arranged on the nitrocellulose membrane in sequence, the conjugation pad is coated with fluorescently labeled PCT antibody complex, biotin Labeled PCT antibody complexes, fluorescently labeled CRP antibody complexes and fluor...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/74G01N33/58G01N33/558
CPCG01N33/6893G01N33/74G01N33/582G01N33/58G01N33/558G01N2333/4737G01N2333/585G01N2800/7095
Inventor 金永敏王怀忠李登红梁健嘉李哉珉崔义烈王洪涛
Owner 巴迪泰(广西)生物科技有限公司
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