Procalcitonin and C-reactive protein combined detection kit
A technology of reacting protein and procalcitonin, which is applied in the biological field, can solve problems such as short reaction time, incomplete reaction, and poor precision, and achieve the effect of saving materials and improving precision
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Embodiment 1
[0051] A method for preparing procalcitonin and C-reactive protein joint detection kit reagent strips, comprising the following steps:
[0052] S1. Screening antibodies and antigens: Purchase CRP detection antibodies, CRP antigens, streptavidin and chicken IgY and perform pairing experiments on them to screen out CRP detection antibodies, CRP antigens, and streptavidin with high specificity and good affinity Chicken IgY and chicken IgY, adjust CRP detection antibody, streptavidin, chicken IgY and CRP antigen to 0.01-5mg / mL, 1-10mg / mL, 0.01-5mg / mL, 0.01-5mg respectively with coating diluent / mL;
[0053] S2. Coating with antibodies and antigens: Prepare the nitrocellulose membrane 5 and correctly place it on the film sticking equipment, and stick the nitrocellulose membrane 5 on the pad card 4 through the film sticking equipment. Spray the chicken IgY adjusted with the coating diluent on the nitrocellulose membrane 5 with a spraying equipment to form a control line, and spray ...
Embodiment 2
[0056] A combined detection kit for procalcitonin and C-reactive protein, the pH of reaction buffer 1 is 7.4, and the components are: 10mM PBS, 200mM NaCl, 0.01% NaN 3 , 0.1% Tween 20, 0.01% IGEPAL CA-630, 1ug / mL fluorescently labeled CRP antibody complex, 0.03ug / mL fluorescently labeled Anti-chicken IgY antibody complex; The first liquid in 7.4 was prepared by freeze-drying. The components of the first liquid were: 10mM PBS, 0.05% BSA, 0.05% Sucrose, 0.01ug / mL biotin-labeled PCT antibody complex, 0.01ug / mL fluorescently-labeled Anti-chickenIgY antibody complex; the second probe 3 is obtained by freeze-drying the second liquid with a pH of 7.4, and the components of the second liquid are: 10mM PBS, 0.05% BSA, 0.05% Sucrose, 2ug / mL fluorescence The labeled PCT antibody complex; the reagent strip includes a pad card 4, a nitrocellulose membrane 5 laid on the pad card 4, a sample pad 6 sequentially arranged on the nitrocellulose membrane 5, a quality control line 7, a A detectio...
Embodiment 3
[0058] A combined detection kit for procalcitonin and C-reactive protein, the pH of reaction buffer 1 is 7.4, and the components are: 10mM PBS, 400mM NaCl, 0.25% NaN 3 , 1.5% Tween 20, 0.25% IGEPAL CA-630, 5ug / mL fluorescently labeled CRP antibody complex, 1.5ug / mL fluorescently labeled Anti-chicken IgY antibody complex; The first liquid in 7.4 was prepared by freeze-drying. The components of the first liquid were: 10mM PBS, 5% BSA, 2.5% Sucrose, 5ug / mL biotin-labeled PCT antibody complex, 5ug / mL fluorescently-labeled Anti- chicken IgY antibody complex; the second probe 3 is obtained by freeze-drying the second liquid with a pH of 7.4, and the components of the second liquid are: 10mMPBS, 5% BSA, 2.5% Sucrose, 11ug / mL fluorescently labeled PCT antibody complex; the reagent strip includes a pad card 4, a nitrocellulose membrane 5 laid on the pad card 4, a sample pad 6 sequentially arranged on the nitrocellulose membrane 5, a quality control line 7, and a first detection Line 8...
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