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Point diffraction digital holographic microscopy device and method based on polarization grating

A technology of digital holographic microscopy and polarization grating, which is applied in the direction of optical devices, measuring devices, and material analysis through optical means. It can solve the problems of fringe contrast that cannot guarantee high interference patterns, achieve high precision, and overcome stability. , the effect of suppressing coherent noise

Active Publication Date: 2020-08-21
陕西范纳尔光电科技有限公司
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Problems solved by technology

However, it should be noted that the existing point-diffraction phase microscopy technology forms reference light by filtering the object light wave, so the light intensity of the reference light is related to the sample to be tested, and it cannot be guaranteed that the interference patterns of all samples are consistent. high fringe contrast

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  • Point diffraction digital holographic microscopy device and method based on polarization grating
  • Point diffraction digital holographic microscopy device and method based on polarization grating
  • Point diffraction digital holographic microscopy device and method based on polarization grating

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Embodiment

[0074] In the experiment, laser 1 is a LQB crystal solid-state laser (λ=532nm, 1875M-532-(10-50)-5V, radium blue laser, Wuhan, China), the exit spot diameter: 4mm; wavelength range: 532nm±5nm (half Height full width 10nm); linear polarization and the polarization direction is the horizontal direction; price: 150RMB. The magnification of the microscope objective 4 is 20X, and the numerical aperture NA=0.4 (plan objective lens, 20×LWD, Nanjing Yingxing, Nanjing, China). The first thin lens 5 focal length f 1 =150mm (AC254-150-A, ThorlabsInc., USA); the focal length of the second thin lens 8 is f 2 =50mm (GCL-010652, Daheng Optoelectronics, Beijing, China). The pinhole diameter of the pinhole filter is 50 μm (GC0-01020, Daheng Optoelectronics, Beijing, China); the period of the grating 7 is Λ=6.3 μm (#12-677, Edmund Optics, Shenzhen, China). Because (1 / Λ=1 / 6.3=0.16μm -1 )>(2ν max =2 / (0.61λ / NA) / M=0.12μm -1 , M is the magnification of the objective lens), so the device can se...

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Abstract

The invention discloses a point diffraction digital holographic microscopy device and method based on a polarization grating. The point diffraction digital holographic microscopy device comprises a laser, a light intensity control unit, a microobjective, a first thin lens, a reflecting mirror, the grating, a second thin lens, a pinhole filter, a polarizing film and a CMOS camera. The invention further discloses the point diffraction digital holographic microscopy method based on the polarization grating. The point diffraction digital holographic microscopy device and method have the advantagesof being high in stability, capable of achieving real-time amplitude / phase imaging and the like, and can be widely applied to the fields of biomedical imaging, industrial detection and the like.

Description

technical field [0001] The invention belongs to the technical field of microscopic imaging, and in particular relates to a point-diffraction digital holographic microscopic device and method based on a polarization grating. Background technique [0002] Since the appearance of the optical microscope in the 17th century, optical microscopy technology has become an important means for people to observe the microscopic world. However, the traditional optical microscope can only obtain the intensity information of the observed sample, but it is powerless for transparent samples. In order to overcome the shortcomings of low imaging contrast of traditional optical microscopes, fluorescence microscopy uses chemical dyes or fluorescent proteins to specifically label cells, which can achieve high-contrast and selective imaging of specific cell structures or functional groups. Therefore, there is a growing desire to develop an imaging technique that can observe cells in their natural...

Claims

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Application Information

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IPC IPC(8): G01B9/04G01B11/24G01N21/45
CPCG01B9/04G01B11/2441G01N21/453
Inventor 郜鹏温凯郑娟娟马英刘立新王宇张美玲
Owner 陕西范纳尔光电科技有限公司
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