InDel marker associated with growth traits of pinctada martensii, detection primer group and development method

A technology of Pinctada martensii, growth traits, applied in biochemical equipment and methods, microbial determination/inspection, bioinformatics, etc., can solve problems such as insufficient utilization of discovery, and achieve the effect of reducing workload

Active Publication Date: 2020-09-01
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In addition, there are still a large number of studies that screen differentially expressed genes through transcriptome sequencing, and analyze the molecular genetic basis of trait formation based on gene expression, but have not yet found that SNPs or InDels in differential genes are fully utilized and associated with traits. report

Method used

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  • InDel marker associated with growth traits of pinctada martensii, detection primer group and development method
  • InDel marker associated with growth traits of pinctada martensii, detection primer group and development method
  • InDel marker associated with growth traits of pinctada martensii, detection primer group and development method

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Experimental program
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Embodiment

[0033]100 individuals were randomly selected from the Shenzhen population of Pinctada martensii, and the attachments on the shells were cleaned. Measure and record the shell length, height, width and body weight of each individual one by one. Then sort the 100 individuals by size according to the shape of the hinge line, take 20% of the individuals ranked before and after each as samples, the top 20% of individuals as the maximum value group (L), and the last 20% of individuals as the minimum value group(s). The mantle tissue was dissected and stored in liquid nitrogen for RNA extraction.

[0034] The fixed tissues of 3 individuals were taken from the L and S groups respectively, the total RNA was extracted, and the transcriptome was sequenced and assembled. Align CleanReads with the reference genome to obtain position information on the reference genome or gene.

[0035] The Pearson Correlation Coefficient r (Pearson Correlation Coefficient) was used as the evaluation inde...

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Abstract

The invention discloses an InDel marker associated with growth traits of pinctada martensii, a detection primer group and a development method. The InDel marker is prepared by inserting or deleting asequence TNNN between the 103rd base and the 104th base of a sequence shown as SEQ ID NO.1 from the 5' end, and the NNN represents unknown bases and the number of the bases. According to the development method, InDel is creatively screened from differential expression genes and is associated with traits, and a molecular marker SNP441 associated with the traits is developed. The detection primer group designed by using the molecular marker SNP441 can be used for genotyping the molecular marker SNP441 of pinctada martensii to be detected, screening out dominant growth individuals as backup parents for breeding, and realizing molecular marker-assisted breeding.

Description

Technical field: [0001] The invention belongs to the technical field of aquatic animal genetics and molecular marker assisted breeding, and specifically relates to an InDel marker associated with the growth traits of Pinctada martensii, a detection primer set and a development method. Background technique: [0002] Pinctada martensii is the main species of pearl culture in my country and the world. Since the successful artificial breeding of Pinctada martensii in my country in 1965, the seawater pearl industry has developed rapidly and has become one of the pillar industries of mariculture in Guangdong, Guangxi and some coastal areas of Hainan. In the past 10 years, the quality and output of seawater pearls in my country have declined significantly, the international competitiveness has weakened, and the economic benefits of breeding have declined. The reason, in addition to the deterioration of the breeding environment and the backward breeding technology, is another impor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12Q1/6809C12N15/11G16B20/20G16B25/20G16B25/10G16B35/20
CPCC12Q1/6888C12Q1/6858C12Q1/6809G16B20/20G16B25/20G16B25/10G16B35/20C12Q2600/124C12Q2600/156C12Q2531/113C12Q2563/107
Inventor 何毛贤张华刘文广石禹
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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