Medicine for preventing and/or treating brain tumor and application of medicine
A drug and brain tumor technology, applied in the field of biomedicine, can solve problems such as inability to improve survival, limited efficacy of brain tumor treatment, unsatisfactory clinical trial results, etc., and achieve the effect of improving infection efficiency and efficacy
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Embodiment 1
[0096] This embodiment provides a method for freezing and resuscitating Plasmodium parasite blood, and the specific operations are as follows:
[0097] After the Plasmodium-infected blood is put into the warehouse, the Plasmodium blood is quickly subpackaged according to the requirements, and the subpackaged Plasmodium blood is labeled and stored in liquid nitrogen.
[0098] (1) Cryopreservation of Plasmodium:
[0099] (1) Preparation of experimental materials:
[0100] Plasmodium: Peripheral blood of malaria patients without other legal blood-borne infectious diseases, or blood of Plasmodium parasites cultured in the laboratory;
[0101] Reagents: sodium chloride injection, 10% NaCl solution, 3.5% NaCl solution, RPMI1640 medium, 28% glycerin freezing solution (mix glycerin 28g, sorbitol 3g, and sodium chloride injection and dilute to 100mL volume);
[0102] Consumables: Cryotubes, 50mL centrifuge tubes, 15mL centrifuge tubes, 250mL medium bottles, Pasteur tubes, printing l...
Embodiment 2
[0118] This embodiment provides a method for freezing, resuscitating and inoculating Plasmodium sporozoites. The specific operations are as follows:
[0119] (1) Blood feeding and sporozoite acquisition by Anopheles mosquitoes
[0120] (1) Anopheles preparation: starve more than 300 Anopheles 24 hours in advance;
[0121] (2) Collect insect blood: detect the density of malaria parasites and gametocytes in volunteers infected with malaria, collect 2 mL of peripheral blood from volunteers intravenously, put it into a thermos cup immediately, and transport it to the mosquito room within 2 hours;
[0122] (3) Blood feeding of Anopheles mosquitoes: Adjust the temperature of the mosquito room to 26°C before blood feeding, prepare the film blood feeding system, add the collected peripheral blood to the film blood feeding system, and feed Anopheles mosquitoes for 30 minutes;
[0123] (4) Breeding of Anopheles mosquitoes: absorb unsatisfied Anopheles mosquitoes, write labels, put Anop...
Embodiment 3
[0144] This example explores the therapeutic effect of Plasmodium infection on tumor-bearing mice in a subcutaneous brain tumor inoculation model.
[0145] (1) Experimental materials and reagents:
[0146] Animals: C57BL / 6 mice, female, 6-8 weeks old, from Shanghai Slack Experimental Animal Co., Ltd.; Plasmodium: mouse Plasmodium yoelii (P.yoelii 17XNL, MRA-593, Py), from Free gift from Malaria Research and Reference Reagent Resource Center (MR4); microscope: Olympus microscope CX31; vernier caliper: electronic digital display oil-scaling caliper, item number 678040, purchased from Kaifeng Tools; Giemsa dye powder: purchased from Sigma-Aldrich Company; Pathological grade glass slide: Cat. No. 10127101P-G, purchased from Jiangsu Shitai Experimental Equipment Co., Ltd.; cedar oil: China Shanghai Specimen Model Factory; PBS buffer: SH30256.01, purchased from Hyclone Company; normal saline: purchased from Chenxin Pharmaceutical Co., Ltd.
[0147] (2) Experimental steps
[0148]...
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