Medicine for preventing and/or treating brain tumor and application of medicine

A drug and brain tumor technology, applied in the field of biomedicine, can solve problems such as inability to improve survival, limited efficacy of brain tumor treatment, unsatisfactory clinical trial results, etc., and achieve the effect of improving infection efficiency and efficacy

Pending Publication Date: 2020-09-04
BLUE ELEGANT BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Due to the limited efficacy of the three traditional therapies for brain tumors, the results of clinical trials of existing immunotherapies, such as targeted therapy and immune checkpoint inhibitors, are unsatisfactory and cannot improve survival

Method used

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  • Medicine for preventing and/or treating brain tumor and application of medicine
  • Medicine for preventing and/or treating brain tumor and application of medicine
  • Medicine for preventing and/or treating brain tumor and application of medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] This embodiment provides a method for freezing and resuscitating Plasmodium parasite blood, and the specific operations are as follows:

[0097] After the Plasmodium-infected blood is put into the warehouse, the Plasmodium blood is quickly subpackaged according to the requirements, and the subpackaged Plasmodium blood is labeled and stored in liquid nitrogen.

[0098] (1) Cryopreservation of Plasmodium:

[0099] (1) Preparation of experimental materials:

[0100] Plasmodium: Peripheral blood of malaria patients without other legal blood-borne infectious diseases, or blood of Plasmodium parasites cultured in the laboratory;

[0101] Reagents: sodium chloride injection, 10% NaCl solution, 3.5% NaCl solution, RPMI1640 medium, 28% glycerin freezing solution (mix glycerin 28g, sorbitol 3g, and sodium chloride injection and dilute to 100mL volume);

[0102] Consumables: Cryotubes, 50mL centrifuge tubes, 15mL centrifuge tubes, 250mL medium bottles, Pasteur tubes, printing l...

Embodiment 2

[0118] This embodiment provides a method for freezing, resuscitating and inoculating Plasmodium sporozoites. The specific operations are as follows:

[0119] (1) Blood feeding and sporozoite acquisition by Anopheles mosquitoes

[0120] (1) Anopheles preparation: starve more than 300 Anopheles 24 hours in advance;

[0121] (2) Collect insect blood: detect the density of malaria parasites and gametocytes in volunteers infected with malaria, collect 2 mL of peripheral blood from volunteers intravenously, put it into a thermos cup immediately, and transport it to the mosquito room within 2 hours;

[0122] (3) Blood feeding of Anopheles mosquitoes: Adjust the temperature of the mosquito room to 26°C before blood feeding, prepare the film blood feeding system, add the collected peripheral blood to the film blood feeding system, and feed Anopheles mosquitoes for 30 minutes;

[0123] (4) Breeding of Anopheles mosquitoes: absorb unsatisfied Anopheles mosquitoes, write labels, put Anop...

Embodiment 3

[0144] This example explores the therapeutic effect of Plasmodium infection on tumor-bearing mice in a subcutaneous brain tumor inoculation model.

[0145] (1) Experimental materials and reagents:

[0146] Animals: C57BL / 6 mice, female, 6-8 weeks old, from Shanghai Slack Experimental Animal Co., Ltd.; Plasmodium: mouse Plasmodium yoelii (P.yoelii 17XNL, MRA-593, Py), from Free gift from Malaria Research and Reference Reagent Resource Center (MR4); microscope: Olympus microscope CX31; vernier caliper: electronic digital display oil-scaling caliper, item number 678040, purchased from Kaifeng Tools; Giemsa dye powder: purchased from Sigma-Aldrich Company; Pathological grade glass slide: Cat. No. 10127101P-G, purchased from Jiangsu Shitai Experimental Equipment Co., Ltd.; cedar oil: China Shanghai Specimen Model Factory; PBS buffer: SH30256.01, purchased from Hyclone Company; normal saline: purchased from Chenxin Pharmaceutical Co., Ltd.

[0147] (2) Experimental steps

[0148]...

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Abstract

The invention relates to a medicine for preventing and / or treating brain tumor and application of the medicine. The medicine comprises plasmodium. The medicine has a remarkable prevention and / or treatment effect on the brain tumor, can inhibit growth of the brain tumor and prolong the life of a patient, has no obvious toxic or side effect and is high in safety. The medicine enriches the treatmentmeans of the brain tumor, and due to the specificity and sensitivity of brain tissues, chemotherapeutic drugs cannot penetrate through the blood-brain barrier easily and cannot play an anti-tumor effect. The medicine breaks through the blood-brain barrier by mainly adjusting the immune function of the organism and inflammation related factors and comprehensively activating the immune capacity, growth of the brain tumor is inhibited, and therefore, the life is prolonged.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a medicine for preventing and / or treating brain tumors and its application. Background technique [0002] Brain tumors are clinically common tumors that seriously threaten human health and survival. Brain tumors are divided into primary brain tumors and metastatic brain tumors. Metastatic brain tumors are more common than primary brain tumors. Among the metastatic brain tumors, lung cancer is the most common, followed by breast cancer and melanoma, accounting for about 67-80% of the total brain metastases. The most common primary brain tumors are glioma, meningioma, pituitary adenoma, and nerve sheath tumor, among which glioma accounts for 40% to 50% of primary tumors. Accounts for 75% of malignant primary brain tumors. According to the grading system formulated by WHO, gliomas are divided into grade 1 (the lowest degree of malignancy and the best prognosis) to...

Claims

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Application Information

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IPC IPC(8): A61K35/68A61P35/00A61P35/04
CPCA61K35/68A61P35/00A61P35/04Y02A50/30
Inventor 陶铸丁文婷程智鹏冯银芳秦莉陈小平
Owner BLUE ELEGANT BIOTECH CO LTD
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