Polyionic liquid modified nano material, preparation method thereof and application of material in enrichment of phosphorylated peptides

A technology of polyionic liquids and nanomaterials, applied in the enrichment of phosphorylated peptides, in the field of polyionic liquids modified nanomaterials, can solve the problems of enrichment of phosphorylated peptides, etc., to increase practicability and ease Separate, easy-to-handle effects

Active Publication Date: 2020-09-04
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing materials still cannot fully meet the needs of enriching phosphorylated peptides from complex samples in terms of specific selectivity, enrichment capacity, acid and alkali resistance stability, anti-interference and reusability in the enrichment process of phosphorylated peptides

Method used

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  • Polyionic liquid modified nano material, preparation method thereof and application of material in enrichment of phosphorylated peptides
  • Polyionic liquid modified nano material, preparation method thereof and application of material in enrichment of phosphorylated peptides
  • Polyionic liquid modified nano material, preparation method thereof and application of material in enrichment of phosphorylated peptides

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Experimental program
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Effect test

Embodiment 1

[0022] Embodiment 1: wherein E=nSiO 2 .

Embodiment 2

[0023] Embodiment 2: E=Fe wherein 3 o 4 @nSiO 2 .

Embodiment 3

[0024] Embodiment 3: where E=G@mSiO 2 .

[0025] Among the above three implementations, the difference lies in the difference of the base material E, and the preparation method is the same: firstly modify the vinyl monomer on the surface of the base, and then use 3-(3-(diethoxyphosphate) propyl)-1- Vinylimidazolium bromide and the vinyl group on the surface of the substrate are polymerized to obtain E-PPFIL modified with organic phosphonic acid groups; after acidification treatment, metal ions are immobilized on the organic phosphonic acid groups to obtain fixed metal ion Adsorbent E-PPFIL-Ti 4+ .

[0026] Its preparation method is as follows.

[0027] (1) Substrate material surface modification vinyl monomer:

[0028] 200 mg of base material was dispersed in 20 mL of anhydrous toluene. After ultrasonic dispersion, 220 μL of vinyltriethoxysilane and 22 μL of triethylamine were added thereto, and reacted at 110° C. overnight. Centrifuge or magnetically separate the solid m...

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Abstract

The invention discloses a polyionic liquid modified nano material, a preparation method thereof and application of the material in enrichment of phosphorylated peptide in a sample containing phosphorylated peptide. The preparation method comprises the following steps: (1) polymerizing a vinyl-modified substrate material, vinyl and organic phosphonate functionalized ionic liquid to obtain a polyorganic phosphonate ionic liquid functionalized material; and (2) acidifying and hydrolyzing the polyorganic phosphonate ionic liquid functionalized material, and then combining with metal ions to obtainthe affinity material of the polyorganic phosphonate functionalized ionic liquid. The functionalized material prepared by the method is easy to separate, has better hydrophilicity, metal ion immobilization capability, acid and alkali resistance, stability and good specific selectivity, and is suitable for enrichment and purification of phosphorylated peptides of complex biological samples. The material has a wide application prospect in the field of biomedicine.

Description

technical field [0001] The invention belongs to the field of functional materials, and specifically relates to a polyionic liquid modified nanometer material, and also relates to a preparation method of the material and its application in enriching phosphorylated peptides. Background technique [0002] The Human Genome Project launched in 1990 greatly promoted the development of life-related disciplines, leading to the introduction of the concept of proteomics in 1994. Proteomics is the study of protein structure characteristics, expression, modification and related functions. Protein phosphorylation is an important post-translational modification. Research on the mechanism of protein phosphorylation-dephosphorylation can provide valuable information for physiological processes, discovery of clinical markers, occurrence and development of diseases, and drug development and design . [0003] Although mass spectrometry has high sensitivity, it is difficult to directly use bi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/22B01J20/28B01J20/286B01J20/30C07K1/22
CPCB01J20/223
Inventor 王斌斌梁洪泽蒋雨菲梁炜达刘彬蒋银丽卢俐衡林嘉宇桑坤明黎思蕴
Owner NINGBO UNIV
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