Open type tissue culture rooting method for actinidia arguta

A soft jujube kiwifruit and open technology is applied in the open tissue culture rooting field of soft jujube kiwifruit to achieve the effects of reduced workload, good appearance and reduced cost

Active Publication Date: 2020-09-11
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports about the open culture of Actinidia jujube at the rooting stage

Method used

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  • Open type tissue culture rooting method for actinidia arguta
  • Open type tissue culture rooting method for actinidia arguta
  • Open type tissue culture rooting method for actinidia arguta

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Add 30 mL of 1 / 4MS sugar-free solid medium with a thickness of 2 cm in a culture bottle, add 9 mg of tetrachloroisophthalonitrile and 0.3 μg of auxin IBA, and set aside;

[0028] (2) 5 strains of Actinidia jujuba tissue culture seedlings were inoculated into the medium containing bacteriostatic agent in step (1) and cultivated. The cultivation temperature was 22° C., and light was given. The light intensity was 1800 lx, and the light time was 12 hours per day. 20 days.

Embodiment 2

[0030] (1) Add 30 mL of 1 / 4MS sugar-free solid medium with a thickness of 2 cm in a culture bottle, add 6 mg of tetrachloroisophthalonitrile and 1.5 μg of auxin IBA, and set aside;

[0031] (2) 6 strains of Actinidia jujuba tissue culture seedlings were inoculated into the medium containing bacteriostatic agent in step (1) and cultivated, the cultivation temperature was 20°C, and light was given, the light intensity was 1600lx, and the light time was 14h every day, and cultivated 22 days.

Embodiment 3

[0033] (1) Add 30 mL of 1 / 4MS sugar-free solid medium with a thickness of 2.5 cm in a culture bottle, add 15 mg of tetrachloroisophthalonitrile and 3 μg of auxin IBA, and set aside;

[0034] (2) Inoculate 4 strains of Actinidia jujuba tissue culture seedlings into the medium containing bacteriostatic agent in step (1) and cultivate them. The cultivation temperature is 24°C, and light is given. The light intensity is 2000lx, and the light time is 10h every day. 18 days.

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Abstract

The invention discloses an open type tissue culture rooting method for actinidia arguta. The method comprises the following steps: adding a bacteriostatic agent into a culture medium for later use; and inoculating actinidia arguta tissue culture seedlings into the culture medium containing the bacteriostatic agent for culturing. According to the method disclosed by the invention, the bacteriostatic agent tetrachloroisophthalonitrile is added into the culture medium, and the auxin concentration required by open type tissue culture rooting of the actinidia arguta and the adding concentration ofthe tetrachloroisophthalonitrile in the culture medium are limited, so that the technical aim of open type tissue culture rooting of the actinidia arguta is achieved. Therefore, when the actinidia arguta is subjected to tissue culture rooting, a good rooting effect can be achieved without a strict sterile operating environment, high-pressure sterilization and an ultra-clean workbench, so that theoperation can be simplified, the workload is reduced, and the cost of tissue culture industrialized seedling culture is obviously reduced.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to an open tissue culture and rooting method for Actinidia jujube. Background technique [0002] At present, the tissue culture technology of Actinidia jujube has matured day by day, but the traditional tissue culture requires strict environmental conditions, and the operation is more complicated, which undoubtedly increases the cost. The open tissue culture is to add bacteriostatic agents to the culture medium, so that the plant tissue culture can be separated from the strict aseptic operating environment, without the need for high pressure sterilization and ultra-clean workbench, and the plant can be carried out in a natural and open environment with bacteria. Advanced tissue culture reduces environmental requirements, reduces operating links, and significantly reduces costs in terms of equipment, venues, and energy. Open tissue culture has been widely used ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 李昌禹秦红艳范书田张宝香赵梓皓杨义明赵滢许培磊王月刘迎雪路文鹏谭天媛
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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