Exosome preparation prepared from umbilical cord mesenchymal stem cells, and preparation method of exosome preparation
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of stem cells and exosomes, applied in the field of cell biology, can solve problems such as the influence of pH and salt concentration, cumbersome steps, and inappropriateness, and achieve the effects of excellent protein expression, increased centrifugation speed, and excellent particle size
Pending Publication Date: 2020-09-11
广州达康基因技术有限公司
View PDF5 Cites 10 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Many of the current various technologies have their drawbacks and are not suitable for clinical applications, including ultracentrifugation (time-consuming, unstable yield, low purity and damage to vesicles), density gradient centrifugation (complex steps...
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0047] Examination and characterization of isolated UC-MSC-derived exosomes. By morphology and plasticity (Figure 1), specific surface antigen (Ag) expression analysis ( figure 2 ) and pluripotent differentiation potential ( image 3 ) According to the criteria of the International Society for Cell Therapy to define mesenchymal stem cells, the source of exosomes was confirmed as MSC (Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop Dj, Horwitz E. Minimalcriteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8(4):315-7.).
[0048] Examination and characterization of isolated UC-MSC-derived exosomes. particle size ( Figure 4A ) and exosome protein expression analysis ( Figure 4B ) results meet the minimum requirements for extracellular vesicles defined by the International Society for Extracellular Vesicles ( J, Hill AF, Hoch...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention relates to an exosome preparation prepared from umbilical cord blood bone marrow mesenchymal stem cells, and a preparation method of the exosome preparation. The exosome is obtained froma cell culture containing at least 10<4> cells. When the cell culture reaches fusion, washing is performed in PBS, a KO-DMEM culture medium is added and the cell culture is put back into an incubatorcontaining carbon dioxide for culture, the cell culture is cultured for 2-20 days, and the exosome is prepared through the ultracentrifugation technology, size exclusion filtration, chemical precipitation, discontinuous density gradient, immunoaffinity, ultrafiltration and/or high performance liquid chromatography, and preferably through PEG precipitation. The exosome prepared by the method is stable, high in purity, and comprehensive in positive markers, and can be used for activating proliferation and regeneration, promoting wound healing, and other medical clinical uses.
Description
technical field [0001] The present invention relates to cell biology, and in particular to a preparation method for producing stable exosomes. Background technique [0002] Exosomes are cell-derived vesicles (Hong, C.S., Muller, L., Boyiadzis, M. and Whiteside, T.L., 2014. Isolation and characterization of CD34+ blast-derived exosomes inacute myeloid leukemia. PloS one, 9(8 ), p.e103310). They are found in biological fluids such as urine, plasma, and ascites. Exosomes are produced by inward budding of endosomal multivesicular bodies. The molecules transported by exosomes include proteins / glycoproteins expressed on the cell membrane as well as molecules and soluble factors present in the parental cell cytosol. Exosomes usually have a diameter of 40–100 nm and contain proteins, lipids, RNA and microRNAs (Zhang, Z., Wang, C., Li, T., Liu, Z. and Li, L., 2014. Comparison of ultracentrifugation and density gradient separation methods for isolating Tca8113 human tongue cancer ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more