46results about How to "Cumbersome steps" patented technology

The invention relates to a method for extracting and purifying anthocyanins from blueberry pomaces. The method comprises the following steps of: mashing blueberries, separating pulps from pomaces, sequentially carrying out enzymolysis, separation, ultra-filtrated impurity removal, nanofiltration concentration and macroporous resin purification on the obtained object, recovering ethanol and an intercepting liquid through nanofiltration, and carrying out freeze drying on the intercepting liquid so as to obtain claret-colored blueberry anthocyanin powder, wherein the anthocyanins content of the blueberry anthocyanin powder is 50-90%. According to the invention, the added value of blueberry resources is improved; the process is not only simple, but also low in operating cost, therefore, the process is suitable for the large-scale production of anthocyanins; the whole process adopts a physical method, no poisonous and harmful solvents are adopted, and the energy consumption is low, therefore, the method effectively protects the environment; and medium-temperature (25-55 DEG C) enzymolysis is adopted, thereby maximumly keeping that anthocyanins not destroyed.

The present application provides methods and compositions for determining accessibility of a DNA modifying agent in genomic DNA.

The invention discloses edible powder tea oil comprising the following components in percentage by weight: 15 to 35 percent of refined tea oil, 40 to 60 percent of maltodextrin, 20 to 40 percent of arabic gum, 0.5 to 2.5 percent of molecular distillation monoglyceride, 0.5 to 2.5 percent of sucrose ester and the balance of water. By adopting a physical pressing method, oil in the tea seeds is extracted; by low-temperature refining, suitable emulsification and homogenization, a convenient and economical spraying drying method is successfully applied to the preparation of the powder tea oil; the process is simple and feasible; the preparation conditions are easily controlled; the edible powder tea oil particles which are obtained by preparation are uniform and similar to spherical shape; the particle size is within 10 to 40 Mum; the refined tea oil content in the powder is 100 to 300mg/g; the micro-encapsulation efficiency reaches over 90 percent; the solubility and the thermal stability are good; the defect of limited application due to the insolubility of the tea oil in water is avoided; and the edible powder tea oil can be widely applied to food, medicine, cosmetics and other industries.

The invention relates to a method for extracting purified anthocyanin from purple sweet potatoes. The method comprises the following steps of cleaning the purple sweet potatoes, dicing, rough grinding and fine grinding into pulp, extracting at a constant temperature, carrying out solid-liquid separation to remove most of starch and the like, removing macromolecules in a solution by an ultrafiltration membrane, and preliminarily purifying; then further purifying filtrate by macroporous resins, eluting with deionized water and ethanol sequentially to obtain eluate, namely a purified solution of anthocyanin, concentrating the purified solution by a nanofiltration membrane, recovering ethanol, and vacuum freeze-drying to obtain anthocyanin powder. The content of anthocyanin is 50%-98%. The extraction agent used by the invention is water, pollution-free chemical reagents are used in the extraction process, the membrane and macroporous resins are adopted in the crude extraction and purified extraction and the ethanol is recyclable, thereby reducing pollution; anthocyanin is not destroyed to the maximum extent by medium-temperature extraction, the starch removal by a disc centrifuge, and other processes and anthocyanin is not oxidized via a vacuum freeze-drying method.

The invention relates to an extraction method of total DNA of microorganisms from a soil sample. The extraction method particularly includes following steps: (1) adding a DNA extraction buffer solution to the soil sample and performing repetitive freeze-thawing in liquid nitrogen and hot water bath; (2) adding a protease K, performing a water bath treatment process at 37 DEG C for 30 min; (3) adding a sodium dodecyl solution, performing a water bath treatment process at 65 DEG C for 30 min; (4) performing centrifugation to remove precipitations and collect a supernate; (5) performing extraction with a mixed solution containing phenol, chloroform and isoamylol and performing centrifugation to collect a supernate; (6) performing extraction with a mixed solution containing chloroform and isoamylol and performing centrifugation to collect a supernate; (7) adding isopropanol to the supernate in the step (6) to performing precipitation and collecting a precipitation after centrifugation; (8) adding ethanol for washing the precipitation and drying the precipitation at the room temperature; (9) adding a TE buffer solution for dissolving the precipitation to obtain a DNA crude extraction; and (10) purifying the DNA crude extraction through an adsorption column, packaging the purified DNA crude extraction and storing the purified DNA crude extraction at -20 DEG C. The extraction method is especially effective when being used in samples from deep soil and dry sand which are less in biomass and in a clayed soil sample. The DNA extraction can be directly used in subsequent molecular biological detection.

The invention discloses a synthesis method of a vitamin D3 intermediate 7-ketocholesteryl acetate, and relates to 7-ketocholesteryl acetate. The method comprises the following steps: adding cholesteryl acetate to a mixed solvent, introducing oxygen as an oxidant, adding a main catalyst and a nonmetal cocatalyst, carrying out a catalytic allylic oxidation reaction, and purifying the obtained reaction product to obtain the vitamin D3 intermediate 7-ketocholesteryl acetate. No complex post-treatment processes are needed; a quaternary ammonium salt is adopted as the cocatalyst, so decomposition of peroxides is effectively promoted; tetrahydrofuran azeotropic water removal is adopted to remove water generated in the decomposition process of the peroxides, so the decomposition is complete, and the reaction is fast and mild; and he main catalyst and the cocatalyst are nonmetal catalysts respectively, and are cheap and easily available, the main catalyst can be reused 5 or more times, the conversion rate and the selectivity are respectively high, and the reaction yield reaches 85% or above, so the synthesis method has the advantages of high efficiency and low cost.

A self-standing liquid package bag provided with flat film valve is provided at a single use form by self-standing a liquid package bag with a film valve having a one-way function without being required to house and fix in another outer package vessel and obstructing the one-way function of the film valve. The self-standing liquid package bag of the invention is formed by protrudingly disposing a flat film valve having a one-way function, which is subjected to a wetting treatment at full inner faces in a pouring path and a portion adjacent to a fused part near to the pouring path, on an upper part or top part of at least one side edge of a bag body portion and disposing a self-standable bottom portion at bottom thereof.

The invention relates to a fiber composite material, and particularly relates to a fiber/long-carbon-chain nylon in-situ composite material and a preparation method. The fiber/long-carbon-chain nylon in-situ composite material comprises the components: 1-20wt% of high-performance fiber and 80-99wt% of long-carbon-chain nylon salt, preferably 5-16wt% of high-performance fiber and 84-95wt% of long-carbon-chain nylon salt, wherein the high-performance fiber is selected from at least one of aramid fiber, carbon fiber and fiberglass. The preparing process is simple; and the strength and toughness of the prepared fiber/long-carbon-chain nylon composite material are significantly improved as compared with those of the pure long-carbon-chain nylon prepared by the same process.

The invention relates to an improved animal fat processing technology, and provides a method for removing cholesterol from animal fat. The method comprises the following steps: 1, allowing animal fat I to contact with a fatty acid salt, and stirring to form a precipitate; and 2, separating the precipitate to obtain animal II, wherein the amount of the fatty acid sat is not lower than 5% (w/w) of the weight of the animal fat I.

The invention relates to a preparation method of a hydrophobic abrasion-proof film for an automotive glass. The preparation method is characterized by comprising the following steps: 1, cleaning and drying of a glass substrate, namely cleanly cleaning the glass substrate, then polishing by using cerium oxide polishing powder, then flushing residual cerium oxide by using deionized water, and finally drying a glass surface; 2, preparation of the film, namely after the glass surface is dried, firstly coating a film on the glass surface by using an SiO2 sol solution A to obtain an SiO2 film, drying at normal temperature, then coating a film on the surface of the SiO2 film by using a polyfunctional epoxide resin solution B to obtain a polyfunctional epoxide resin film, and then coating a film on the surface of the epoxide resin film by using a hydrophobing agent solution C to obtain a hydrophobing agent film; 3, curing processing: placing the glass coated with the film in the step 2 into a dustless environment, and standing at 100-150 DEG C for 1-2 hours to cure the film.

Methods and systems for cryogenic preservation of cells.

The invention relates to the field of chemical component purification, and in particular, relates to a 2-acetylpyrazine continuous recrystallization device and method and an application of the device. For solving the problems of low yield caused by low utilization rate of 2-acetylpyrazine and low production efficiency and safety and environmental protection risks caused by intermittent operation in a 2-acetylpyrazine recrystallization method in the prior art, the invention provides the 2-acetylpyrazine continuous recrystallization device and method and the application of the device. A crystallizer is provided with three feed ports, an inlet of a mixed solution containing 2-acetylpyrazine is located above a solvent, 2-acetylpyrazine is crystallized and falls back to the bottom of the crystallizer, and the situation that 2-acetylpyrazine crystals are doped with uncrystallized components, and the purity of 2-acetylpyrazine crystallized products is affected is avoided. Two discharge holes are formed in the upper part and the bottom of the crystallizer, so that solutions with different components can be obtained and are reused after being subjected to different treatments, and continuous recrystallization of 2-acetylpyrazine is realized.

The present disclosure describes a method, an apparatus, and storage medium for recognizing medical image. The method includes obtaining, by a device, a medical image. The device includes a memory storing instructions and a processor in communication with the memory. The method further includes determining, by the device, the medical image through a first recognition model to generate a lesion recognition result used for indicating whether the medical image comprises a lesion; and in response to the lesion recognition result indicating that the medical image comprises a lesion, recognizing, by the device, the medical image through a second recognition model to generate a lesion degree recognition result of the medical image used for indicating a degree of the lesion. Manual analysis and customization of a feature extraction solution are not required, so that the efficiency and accuracy of medical image recognition are improved.

The invention discloses a total synthesis method of optically pure tetrandrine, and belongs to the technical field of drug synthesis. The method comprises the following steps: (1) under the action ofa catalyst (1), carrying out intermolecular Ullmann reaction on a compound (1) and a compound (2) under alkaline and high-temperature conditions to synthesize a compound 3; (2) removing a hydroxyl protecting group from the compound (3) under an acidic condition to synthesize a compound (4); (3) carrying out intramolecular Ullmann reaction on the compound (4) under the action of a catalyst (2) under alkaline and high-temperature conditions to synthesize a compound (5), namely the optically pure tetrandrine. A convergent synthesis strategy is adopted, and only three steps are needed from the compound (1) to the synthesis of optically pure tetrandrine so that the reaction steps are greatly reduced, and the time and the material cost are saved; the yield can be as high as 28.7%-38.9%, and theyield is increased by dozens of times; the target product can be obtained on the gram scale, 1.3 g-1. 5 g of final optically pure product is synthesized, and the method has better industrialization potential.

The invention belongs to the field of metallurgy, and discloses a method for preparing a high-nickel type ternary precursor through ferronickel production conversion and application of the method. The method comprises the following steps of drying laterite-nickel ore, carrying out a pre-reduction reaction, and then carrying out deep reduction smelting, separation and refining to obtain a nickel-iron alloy; adding a sulfur-containing material into the ferro-nickel alloy for blowing, and then adding coke powder and quartz to obtain high-grade matte nickel; adding concentrated sulfuric acid into the high-grade matte nickel for reaction, conducting separation and pressure leaching, and obtaining nickel sulfate; and adding a cobalt source and a manganese source into the nickel sulfate, then adding a reducing agent, a precipitator, water and a complexing agent, and carrying out nucleation reaction and nuclear growth to prepare a high-nickel ternary precursor. According to the method for preparing the high-nickel type ternary precursor through the ferronickel production conversion and the application of the method, on the basis of an original RKEF process, the high-grade matte nickel is prepared by taking the ferronickel which is excessive in yield and low in price as an intermediate, adding the sulfur-containing material and adding a blowing device, and then the high-grade matte nickel is used for producing the nickel sulfate, so that the nickel recovery rate is further increased while the raw material supply pressure of the nickel sulfate is relieved to a great extent.

The invention provides a local voice network distribution method, which comprises the following steps that: network distribution control equipment receives a voice control instruction of a user, and the voice control instruction is an IOT intelligent equipment network distribution instruction; the distribution network control equipment comprises a local voice module and an IOT module; a local voice module of the distribution network control equipment performs voice analysis and communicates with an IOT module of the distribution network control equipment; the IOT module of the distribution network control equipment sends a broadcast packet to the outside through near field communication; according to the method provided by the invention, manual network distribution through a mobile phone APP is not needed, the steps are tedious, the learning cost is high, the problems of low manual network distribution speed, high failure rate and the like are solved, the field layout installation efficiency and the field network distribution speed are effectively improved, the user experience is improved, and the whole network distribution process is more convenient.

The invention relates to a novel refrigerator which solves the problem that a refrigerator of a traditional structure is tedious in step of pouring refrigerated beverages. The novel refrigerator has the advantages that beverages are convenient to pour, the opening frequency of a refrigerator door can be effectively reduced, and cold air loss in the refrigerator is avoided.

The invention discloses a template-free synthesis method of an eggshell-shaped silicon dioxide nanomaterial, belonging to the field of functional materials. The method comprises the following steps: dissolving a certain amount of HSiCl3 and tri-n-propylamine in CH2Cl2, and conducting stirring at room temperature for several hours; and evaporating a formed yellow solution to dryness, and calcining a remaining product in a muffle furnace to obtain eggshell-shaped SiO2. The invention aims to provide a rapid preparation method of hollow eggshell-shaped nanometer SiO2. According to the method, the hollow eggshell-shaped nanometer SiO2 can be formed only by calcining a product of a disproportionation reaction of small molecular silane in the presence of amine at a certain temperature. The method has the advantages of quickness and easiness in amplified synthesis, and is expected to be used for industrially preparing the hollow SiO2 nanomaterial.