A method to improve oil production efficiency and carbon fixation rate of microalgae

A microalgae and high-efficiency technology, which is applied in the field of microalgae biotechnology and carbon emission reduction, can solve the problems that high oil content and high oil production rate are difficult to obtain at the same time, lipid production rate cannot be effectively improved, and lack of stimulation to increase lipid content and other problems , to achieve the effects of improving carbon sequestration efficiency, solving biomass yield and oil production efficiency, and increasing oil content

Active Publication Date: 2021-12-28
CHINA JILIANG UNIV
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  • Application Information

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Problems solved by technology

However, the researchers found that while nitrogen deficiency stimulated the increase of lipid content, it was often accompanied by stagnation of cell growth and reduction of biomass, which would lead to an ineffective increase in lipid yield.
Therefore, it is often difficult to obtain high oil content and high oil production rate at the same time

Method used

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  • A method to improve oil production efficiency and carbon fixation rate of microalgae
  • A method to improve oil production efficiency and carbon fixation rate of microalgae
  • A method to improve oil production efficiency and carbon fixation rate of microalgae

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Experimental program
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Effect test

Embodiment 1

[0025] (1) Sterilize the BG11 medium at 121°C for 30 minutes. After the medium is cooled to room temperature, divide it into 600mL round-bottomed glass bottles, inoculate microalgae, and carry out the first stage of cultivation;

[0026] BG11 medium consists of the following components: NaNO 3 1500mg / L, K 2 HPO 4 ·3H 2 O 40mg / L, MgSO 4 ·7H 2 O75mg / L, Na 2 CO 3 20mg / L, CaCl 2 27mg / L, citric acid monohydrate 6mg / L, ferric ammonium citrate 6mg / L, Na 2 EDTA1mg / L, trace element A 5 Solution 1mL, where, A 5 Consists of: H 3 BO 3 2.86mg / L, MnCl 2 4H 2 O1.81mg / L, ZnSO 4 ·7H 2 O 0.222mg / L, CuSO 4 ·5H 2 O 0.079mg / L, CoCl 2 ·6H 2 O 0.050mg / L, Na 2 MoO 4 2H 2 O 0.39mg / L;

[0027] In the present embodiment, the microalgae algae are passed through 60 CO-γ-ray mutagenesis and can tolerate a volume fraction of 60% CO 2 of Chlorella pyrenoidosa, this microalgae undergoes a high-concentration gradient CO 2 For domestication, the concentration of inoculated microalg...

Embodiment 2

[0032] In this embodiment, the microalgae species have not passed through 60 CO-γ-ray mutagenesis, but after high concentration gradient CO 2 For domesticated Chlorella pyrenoidosa, the concentration of inoculated microalgae is 450mg / L.

[0033] Other steps in this embodiment are the same as in Embodiment 1.

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Abstract

The invention discloses a method for improving the oil production efficiency and carbon fixation rate of microalgae, comprising the following steps: (1) inoculating microalgae in a culture medium, and performing the first stage of cultivation; (2) the first stage of cultivation After the end, a phosphorus source and an iron source are added to the culture medium to carry out the second stage of cultivation, wherein the microalgae is Chlorella pyrenoidosa, and the culture conditions of the whole culture process are: the volume fraction of 15 %CO 2 As a carbon source, the gas supply rate is 0.1 v / v / m; the temperature is 27°C, the pH is 6.0~8.0, the light is 8500Lx, and the light-dark cycle is 16:8. The method of the invention can improve the oil production efficiency and oil content of the microalgae, and at the same time greatly improve the carbon fixation efficiency of the microalgae.

Description

technical field [0001] The invention belongs to the fields of microalgae biotechnology and carbon emission reduction, and in particular relates to a method for improving microalgae oil production efficiency and carbon fixation rate. Background technique [0002] The massive emission of carbon dioxide is one of the main causes of the global greenhouse effect, and the reduction of carbon dioxide emission has become the key to the reduction of greenhouse gas emission in various countries. while controlling CO 2 Emissions focus on controlling CO from fossil fuel combustion 2 , thus controlling the CO produced during coal combustion 2 is currently conducting CO 2 The key to reducing emissions. Capture CO in numerous 2 Among the methods, bioresource carbon sequestration technology has become an international CO 2 It is a cutting-edge research hotspot in the field of emission reduction and new energy development, and has broad application prospects. At the same time, the dep...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/89
CPCC12P7/6436
Inventor 储菲菲
Owner CHINA JILIANG UNIV
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