Method for extracting and purifying anthocyanins from blueberry pomaces

A technology of blueberry pomace and anthocyanin, applied in chemical instruments and methods, organic chemistry, food science, etc., can solve the problems of high price and lack of anthocyanin, achieve good environmental friendliness, increase added value, The effect of low energy

Inactive Publication Date: 2013-09-11
LIM KUM KEE FOODSTUFF CO LTD ANHUI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the situation of a large amount of anthocyanins lacking and high prices, the present invention provides a method for extracting and purifying anthocyanins from blueberry pomace

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The specific operation steps for extracting and purifying anthocyanins from blueberry pomace are as follows:

[0033] (1) Remove the leaves and stems from the blueberries, and then wash them with clean water to remove impurities such as mud and sand; beat them with a beater to obtain blueberry pulp;

[0034] (2) Separation of pulp and residue: the blueberry pulp is separated from the blueberry pulp by using a pulp and residue separator with a filter cloth of 60 mesh or a centrifuge with a filter bag of 60 mesh;

[0035] (3) Enzymolysis: Add 2 times the volume of deionized water to the blueberry pulp, adjust the pH of the blueberry pulp solution to 2.5, and the temperature is 25°C; add 0.01% blueberry pulp mass (m / m) to the blueberry pulp solution Pectinase, mix evenly, and enzymolyze for 2 hours until no pectin is detected in the blueberry pomace solution. The pectin in the blueberry pomace solution was detected by absolute ethanol or 95% ethanol. The specific operati...

Embodiment 2

[0042]Embodiment 2 The specific operation steps of extracting and purifying anthocyanins from blueberry pomace are as follows:

[0043] (1) Beating: remove leaves and stems from blueberries, and then wash with water to remove impurities such as mud and sand; beat with a beater to get blueberry pulp;

[0044] (2) Separation of pulp and residue: the blueberry pulp is separated from the blueberry pulp by a pulp and residue separator with a filter cloth of 300 mesh;

[0045] (3) Enzymolysis: Add 20 times the volume of deionized water to the blueberry pulp, adjust the pH of the blueberry pulp solution to 4.5, and the temperature is 55°C; add 0.3% blueberry pulp mass (m / m) to the blueberry pulp solution Pectinase, mixed evenly, enzymatically hydrolyzed for 6 hours, until no pectin was detected in the blueberry pomace solution. The pectin in the blueberry pomace solution was detected by absolute ethanol or 95% ethanol. The specific operation is to take 2.5 ml of blueberry pomace so...

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PUM

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Abstract

The invention relates to a method for extracting and purifying anthocyanins from blueberry pomaces. The method comprises the following steps of: mashing blueberries, separating pulps from pomaces, sequentially carrying out enzymolysis, separation, ultra-filtrated impurity removal, nanofiltration concentration and macroporous resin purification on the obtained object, recovering ethanol and an intercepting liquid through nanofiltration, and carrying out freeze drying on the intercepting liquid so as to obtain claret-colored blueberry anthocyanin powder, wherein the anthocyanins content of the blueberry anthocyanin powder is 50-90%. According to the invention, the added value of blueberry resources is improved; the process is not only simple, but also low in operating cost, therefore, the process is suitable for the large-scale production of anthocyanins; the whole process adopts a physical method, no poisonous and harmful solvents are adopted, and the energy consumption is low, therefore, the method effectively protects the environment; and medium-temperature (25-55 DEG C) enzymolysis is adopted, thereby maximumly keeping that anthocyanins not destroyed.

Description

technical field [0001] The invention belongs to the technical field of extraction and purification of natural pigments, and in particular relates to an extraction and purification method of anthocyanins. Background technique [0002] Blueberry, also known as blue plum, is an angiosperm. The fruit is blue in color, beautiful, pleasing to the eye, blue, with fine flesh and very small seeds. In addition to conventional sugar, acid and VC, blueberry fruit is rich in V E , V A , V B , SOD, protein, anthocyanins, dietary fiber and K, Fe, Zn, Ca and other mineral elements. It is worth mentioning that its peel is rich in anthocyanins. Blueberries not only have good nutritional and health effects, but also have the functions of preventing brain nerve aging, strengthening the heart, anti-cancer, softening blood vessels, and enhancing immunity. [0003] Anthocyanin, also known as anthocyanin, is a kind of water-soluble natural pigment widely present in plants in nature, and belon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C07D311/62
CPCC09B61/00A23L5/43A23L33/105
Inventor 孙汉巨张曼莉杨帆王晓莉王宵李航林明传
Owner LIM KUM KEE FOODSTUFF CO LTD ANHUI
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