Fluorescent probe for detecting hypochlorous acid/sodium hypochlorite as well as preparation method and application thereof

A technology for detecting hypochlorous acid and sodium hypochlorite, applied in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of broken samples and difficult detection, etc., and achieve simple synthesis process and high sensitivity sexual and selective effects

Active Publication Date: 2020-10-02
TAIYUAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] For the traditional detection of HClO / ClO - The existence of the method will cause the damage of the sample to be tested and it is difficult to apply to HClO / ClO in vivo - The technical problem of detection, the invention provides a kind of fluorescent probe that detects hypochlorous acid / sodium hypochlorite and preparation method and application

Method used

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  • Fluorescent probe for detecting hypochlorous acid/sodium hypochlorite as well as preparation method and application thereof
  • Fluorescent probe for detecting hypochlorous acid/sodium hypochlorite as well as preparation method and application thereof
  • Fluorescent probe for detecting hypochlorous acid/sodium hypochlorite as well as preparation method and application thereof

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Embodiment 1

[0035] A fluorescent probe for rapid detection of hypochlorous acid / sodium hypochlorite, the fluorescent probe is 9-(10-ethyl-10H-phenothiazine)-3,6-bis(diethylamino)xanthenium chloride, The structural formula is as formula (1):

[0036]

[0037] The preparation method of the novel fluorescent probe detected in the above-mentioned present embodiment, its synthetic route is as follows: figure 1 shown, including the following steps:

[0038] (1) 10-ethyl-10H-phenothiazine whose synthetic structural formula is shown in formula (2):

[0039]

[0040] The reaction equation is shown in formula (1)

[0041]

[0042] The specific process is: a mixture of phenothiazine (2.0g, 0.010mol) and DMF (30mL) was stirred and dissolved completely under N2, then NaH (1.2g, 0.050mol) was slowly added within 30min, and then 1-bromo Ethane (0.88 mL, 0.012 mol). The mixture was heated at 80 °C for 3 h (TCL). When complete, the solution was poured into 100 g of ice water and collected thro...

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Abstract

The invention belongs to the technical field, and particularly relates to a fluorescent probe for detecting hypochlorous acid / sodium hypochlorite as well as a preparation method and application thereof. The preparation method comprises the following steps: reacting 10H-phenothiazine with bromoethane to obtain 10-ethyl-10H-phenothiazine; carrying out a Vilsmeier reaction on the 10-ethyl-10H-phenothiazine to obtain 3-formyl-10-ethyl-10H-phenothiazine; reacting 3-diethylamino phenol with the 3-formyl-10-ethyl-10H-phenothiazine to obtain 9-(10-ethyl-10H-phenothiazine)-3,6-bis(diethylamino) xanthylium chloride, namely the fluorescent probe for detecting hypochlorous acid / sodium hypochlorite disclosed by the invention. The fluorescent probe is used for detecting hypochlorous acid / sodium hypochlorite in living cells, the hypochlorous acid / sodium hypochlorite fluorescent probe is mixed with a phosphate buffer solution and a DMF buffer solution and then added into a to-be-detected solution to obtain a mixed solution, and whether hypochlorous acid / sodium hypochlorite exists or not is detected through the change of 596 nm fluorescence intensity.

Description

technical field [0001] The invention belongs to the technical field, and in particular relates to a fluorescent probe for detecting hypochlorous acid / sodium hypochlorite, a preparation method and application thereof. Background technique [0002] Reactive oxygen species (ROS) are closely related to various physiological and pathological processes. Extensive studies have shown that an imbalance between reactive oxygen species and antioxidant defense systems in organisms leads to oxidative stress, which may increase the incidence of cancer. As an important organelle to provide energy for the body, mitochondria are the main source of cellular reactive oxygen species, including hydrogen peroxide (H 2 o 2 ), superoxide anion (O 2- ), hydroxyl radicals (·OH), hypochlorous acid (HClO), etc. As an important biological weakly acidic substance, HClO (pK a =7.53) Partially dissociates into ClO at physiological pH - . There is increasing evidence that HClO / ClO - Plays a leading ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D417/04C09K11/06G01N21/64
CPCC07D417/04C09K11/06C09K2211/1037C09K2211/1088G01N21/6402G01N21/6428G01N21/6458
Inventor 赵云薛园园董金龙
Owner TAIYUAN NORMAL UNIV
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