Poneurosis stent and preparation method and application thereof

A membrane scaffold and solution technology, applied in medical science, tissue regeneration, surgery, etc., can solve problems affecting the physical and mental health of patients

Inactive Publication Date: 2020-10-27
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method is the choice after other treatment methods fail, and it belongs to the last step of clinical treatment. Due to the characteristics of easy fibrosis an...

Method used

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  • Poneurosis stent and preparation method and application thereof
  • Poneurosis stent and preparation method and application thereof
  • Poneurosis stent and preparation method and application thereof

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preparation example Construction

[0051]In the first aspect, the present invention provides a method for preparing an aponeurotic scaffold, comprising the following steps: breaking the aponeurotic cells, then breaking the nucleus, removing deoxyribonucleic acid by DNase, and finally preparing the aponeurotic scaffold by slicing.

[0052] The aponeurosis of the present invention is derived from animal aponeurosis tissue, such as porcine aponeurosis tissue, sheep aponeurosis tissue and bovine mucosal tissue. The preparation method of the aponeurosis support of the present invention uses animal aponeurosis tissue as a raw material, first the aponeurotic cells are broken, and the cell membrane is removed; then the nucleus is broken, the nuclear membrane and most of the nucleic acid are removed; and the residual deoxyribonucleic acid is removed by DNase, Avoid exogenous deoxyribonucleic acid or protein contact with the wound to cause immune rejection; finally slice the aponeurosis to obtain aponeurotic scaffold with...

Embodiment 1

[0092] A preparation method of aponeurosis support, comprising the following steps:

[0093] Select fresh pig limbs, and remove the pig's aponeurosis tissue under aseptic conditions, such as figure 1 As shown in the picture on the left (native), cut it into a size of about 2cm*1cm, put it in a 15mL sterile centrifuge tube, rinse with heparin PBS buffer for 30 minutes, and shake it in a laboratory shaker with the speed set at 80 rpm, and the temperature was set at 4 °C. The oscillating liquids are as follows: 2% TritonX-100 solution for 24 hours, sterile PBS solution for 30 minutes, and 0.25% SDS solution for 24 hours. After shaking, rinse with sterile PBS solution for 30 minutes, digest with 1 mg / mL DNase at 37°C for 1 hour, and rinse with sterile PBS solution for 30 minutes. Place in a cryostat, set the slice thickness to 200 microns, and cut the tissue into 200 microns thick slices. Rinse with sterile PBS solution for 10 minutes to prepare the aponeurotic scaffold, such...

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Abstract

The invention provides an aponeurosis stent and a preparation method and an application thereof. The preparation method of the aponeurosis stent comprises the following steps: rupturing aponeurosis cells, rupturing nuclei, removing deoxyribonucleic acid by a DNA enzyme, and finally performing slicing to prepare the aponeurosis stent. The preparation method is simple and quick, low in cost and involves no harmful chemical substances. The aponeurosis stent has good bioactivity, no immunologic rejection and good flexibility, and can effectively prevent cells from penetrating and avoids tendon adhesion.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to an aponeurotic support and its preparation method and application. Background technique [0002] With the development of social mechanization and the popularization of sports competition, the incidence of tendon injury is increasing year by year, causing a huge economic and social burden. According to statistics, tendon diseases account for 19.2% of skeletal musculoskeletal diseases, as high as 30-40% of flexor tendon injuries occur tendon adhesions, and about 9 million operations per year are caused by tendon adhesions. Tendon adhesions manifest as the loss of the sliding function of the tendon and the limitation of the movement function of the adjacent joints, which seriously affects the quality of life and working ability of patients. In clinical practice, tendon adhesions are often treated with release surgery, supplemented by biomaterials or drug treatment, but the tre...

Claims

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Application Information

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IPC IPC(8): A61L31/14A61L31/00
CPCA61L31/005A61L31/14A61L2430/40
Inventor 范存义崔昊旻余雅玲文根陈帅
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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