A kind of preparation method of momordin Ib
An aqueous solution, column chromatography technology, applied in the direction of steroids, organic chemistry, etc., can solve the problems of inability to meet the application, difficult to realize the industrial production of momordinIb, etc., and achieve the effect of achieving a large amount of acquisition and simple separation and purification operations.
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[0037] The invention provides a kind of preparation method of momordin Ib, comprising:
[0038] 1) Mixing the plant extract containing the triterpene saponin derivative of the structural unit of formula (I) or the plant extract of the triterpene saponin derivative of the structural unit of formula (I) with acid to obtain the extract,
[0039]
[0040] Among them, R 1 , R 2 , R 3 , R 4 independently selected from polysaccharide groups, five-carbon sugar groups or six-carbon sugar groups connected to each other by hydrogen, five-carbon sugars and / or six-carbon sugars, and R 1 , R 2 , R 3 , R 4 not simultaneously hydrogen;
[0041] 2) adjusting the pH of the extract in step 1) to 6.5-7.5 with alkali, and then separating by column chromatography to obtain a sample containing momordin Ib;
[0042] 3) The obtained sample containing momordin Ib is recrystallized to obtain the pure product of momordin Ib.
[0043] According to the present invention, the present invention w...
Embodiment 1
[0054] Take 10kg of dried light-leaved flower branches and leaves plant samples rich in triterpene saponin derivatives (including but not limited to compounds such as momordin Ic) with structural units of formula (I), cross 20 mesh sieves after crushing, add 30 liters of 0.05Mol / L Heat dilute sulfuric acid aqueous solution to reflux and extract 3 times (9, 1, 1 hour), filter the extract while it is hot, combine the 3 extracts and adjust the pH value to 7.0 with dilute NaOH lye, then put it on the medical activated carbon (1000g) column, Elute 30 column volumes with water and 40% ethanol aqueous solution, recover the ethanol in the obtained eluent and discard the impurities, then elute 10 column volumes with 85% ethanol aqueous solution, combine the eluents and recover ethanol , to obtain 43.4 g of a momordin Ib sample with a mass content of 42%. After dissolving 43.4g mass content 42% momordin Ib sample, mix sample with the alumina of mass ratio 1: 1, go up alumina (1085g) col...
Embodiment 2
[0058] Take 10kg of dry light-leaved flower branches and leaves plant samples rich in triterpene saponin derivatives (including but not limited to compounds such as momordin Ic) possessing the structural unit of formula (I), cross 20 mesh sieves after pulverizing, add 30 liters of 0.12Mol / L trifluoroacetic acid aqueous solution heated and refluxed for 3 times (9, 1, 1 hour), filtered the extract while it was hot, combined the 3 extracts and adjusted the pH value to 7.0 with dilute NaOH lye, then applied D101 macroporous resin (10kg ) column, eluted 30 column volumes with water and 40% ethanol aqueous solution successively, reclaimed ethanol in the eluent obtained and discarded this part of impurities, then eluted 40 column volumes with 50% ethanol aqueous solution, and combined the eluted parts Liquid and reclaim ethanol, obtain mass content 51% momordin Ib sample 46g. After 46g mass content 51% momordin Ib sample is dissolved, mix sample with the alumina of mass ratio 1: 1, ...
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