Construction method of animal and cell models for improving rosacea inflammation by artemisinin

Abstract

The invention discloses a construction method of animal and cell models for improving rosacea inflammation by artemisinin, and relates to the technical field of skin disease medicaments, in particularto a construction method of animal and cell models for improving rosacea inflammation by artemisinin. The method comprises the following steps of: (1) constructing an animal model for improving rosacea inflammation phenotype by artemisinin; and (2) constructing a cell model for improving rosacea inflammatory phenotype by artemisinin. The invention has the advantages that a potential new drug (artemisinin) for treating rosacea is discovered; the pathogenesis of the rosacea is explored; it is found that artemisinin can obviously improve LL37-induced rosacea-like inflammatory mouse skin lesion erythema, histopathological changes and increase of IL-1 beta, IL-6, TNF alpha and TLR2; meanwhile, artemisinin can reduce infiltration of skin lesion CD4 + T cells, macrophages and neutrophils and inhibit expression of immune cell related chemokines (CXCL10, CCL20, CCL2 and CXCL2) in skin lesion.

Description

technical field [0001] The invention relates to the technical field of medicaments for skin diseases, in particular to a method for constructing animal and cell models for artemisinin to improve rosacea inflammation. Background technique [0002] Rosacea is a chronic facial skin inflammatory disease, mainly manifested as recurrent paroxysmal flushing, persistent erythema, telangiectasia, papules and pustules, nasal hypertrophy and hyperplasia at the prominence of the face. Rosacea mainly occurs in light-skinned people, and epidemiological surveys show that the prevalence of rosacea varies from 2% to 22% in different countries. At present, the detailed pathogenesis of rosacea has not been fully elucidated, but many factors, including ultraviolet rays, diet, heat stimulation, microorganisms and stress state, are believed to induce or aggravate rosacea. Although rosacea does not threaten the life of patients, because the skin lesions are located on the face and are often accom...

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies in the prior art, the present invention provides a method for constructing artemisinin-improved rosacea inflammation animal and cell models, which solves the problem in the above-mentioned background technology that the therapeutic effect of artemisinin on rosacea is not yet clear and requires Establish a mouse model of rosacea for drug intervention to clarify the effect of artemisinin on the inflammatory phenotype and pathological changes of rosacea. Although artemisinin has anti-inflammatory and anti-angioproliferative effects, how to exert these effects in rosacea, specifically The mechanism is not yet clear, so it is necessary to detect cell-related proteins and cytokines at the cellular level through various technical means, and then study the mechanism of action of these molecules, target cells, etc., because it is a reverse research process, so In the early stage, it required a lot of manpower and material resources to carry out large-scale screening work

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