Monoclonal antibody capable of aiming at SARS-CoV-2 spike protein non RBD (Receptor Binding Domain), and application of monoclonal antibody

A monoclonal antibody and spike protein technology, which is applied in the fields of molecular biology and cellular immunology, can solve problems such as the listing of therapeutic drugs and vaccines, and the specificity of new coronaviruses, and achieve weakened virus toxicity, strong specificity, and high potency Effect
CN111961133AActive Publication Date: 2020-11-20潍坊市第二人民医院
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
潍坊市第二人民医院
Publication Date
2020-11-20

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Abstract

The invention discloses a monoclonal antibody capable of aiming at a SARS-CoV-2 spike protein non RBD (Receptor Binding Domain), and application of the monoclonal antibody. The monoclonal antibody isin specific binding with the SARS-CoV-2 spike protein non RBD and comprises complementarity-determining regions, including CDRH1, a CDRH2 and a CDRH3, of a variable region of heavy chain, and the complementarity-determining regions, including a CDRL1, a CDRL2 and a CDRL3, of a variable region of light chain. The monoclonal antibody capable of aiming at the SARS-CoV-2 spike protein non RBD has hightiter and high specificity, can realize efficient expression, can carry out the specific binding with regions, except the spike protein RBD, on the surface of the SARS-CoV-2, is used for qualitatively and quantitatively detecting the SARS-CoV-2, can neutralize certain SARS-CoV-2 virulence and weaken the SARS-CoV-2 virulence, and performs a function of preventing and / or treating SARS-CoV-2 pneumonia.
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Description

technical field

[0001] The invention relates to the technical fields of cellular immunology and molecular biology, in particular to a monoclonal antibody against the non-RBD region of the spike protein of the new coronavirus SARS-CoV-2 and its application. Background technique

[0002] The new coronavirus SARS-CoV-2, which belongs to the Coronaviridae family (Coronaviridae), the betacoronavirus genus (Coronavirus) of the Orthocoronavirinae subfamily (Orthocoronavirinae), the coronavirus associated with severe acute respiratory syndrome (SARS-CoV) and the Middle East Respiratory Syndrome-associated coronavirus (MERS-CoV) close relatives can cause severe pneumonia symptoms. Common clinical symptoms after human infection with the new coronavirus mainly include fever, fatigue, dry cough as the main respiratory symptom, and gradually dyspnea, acute respiratory distress in severe cases, metabolic acidosis that is difficult to correct, and coagulation dysfunction, etc.

[0003] Th...

Claims

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