Method for pretreating hair sample by using dry-wet mixing method

A technology of sample pretreatment and mixing method, applied in the preparation of test samples, etc., can solve problems such as inability to transform elements, and achieve good applicability, simplified measurement process, and good stability

Inactive Publication Date: 2020-12-08
安徽新生医学科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The organic matter dissolution method requires a specific organic solvent, which cannot effectively transform trace elements in the hair sample into inorganic matter

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The present invention proposes a method for pretreatment of hair samples by wet-dry mixing method, comprising:

[0029] 1. Reagents and materials

[0030] Hair, nitric acid (analytical pure), hydrogen peroxide (analytical pure), first grade deionized water, neutral detergent.

[0031] 2. Instruments and equipment

[0032] Fume hood, temperature-controlled electric heating plate, ultrasonic cleaning machine, electronic balance (accuracy 0.0001g), blast drying oven, pipette, 25ml volumetric flask, watch glass.

[0033] Specific steps are as follows:

[0034] Step 1), hair sample washing

[0035] Take the occipital hair 1cm away from the scalp, cut about 0.5g of hair about 3cm long (discarded at the far end), put it into a numbered 50ml beaker, add 30ml of washing liquid containing 5% neutral detergent into the beaker, and then put Wash in an ultrasonic cleaner for 5 minutes, multiple samples can be carried out at the same time, pour off the washing liquid, repeat the ...

Embodiment 2

[0051] The present invention proposes a method for pretreatment of hair samples by wet-dry mixing method, comprising:

[0052] 1. Reagents and materials

[0053] Hair, nitric acid (analytical pure), hydrogen peroxide (analytical pure), first grade deionized water, neutral detergent.

[0054] 2. Instruments and equipment

[0055] Fume hood, temperature-controlled graphite furnace with holes, ultrasonic cleaning machine, electronic balance (accuracy 0.0001g), blast drying oven, pipette, 10ml glass graduated tube (supporting graphite furnace).

[0056] Specific steps are as follows:

[0057] Step 1), hair sample washing

[0058]Take a hair sample Take the occipital hair 1cm away from the scalp, cut about 0.2g of hair about 3cm long (discarded at the far end), put it into a numbered 10ml graduated tube, add 10ml of 5% neutral detergent to the graduated tube Washing solution, and then put it into an ultrasonic cleaner for 5 minutes to clean multiple samples at the same time, po...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for pretreating a hair sample by using a dry-wet mixing method, which comprises the following steps: step 1, cleaning the hair sample, step 2, drying the hair sample,step 3, weighing the hair sample, step 4, digesting the hair sample and removing acid, and step 5, fixing the volume. In the invention, a low-temperature normal-pressure steam seasoning method is adopted for digesting the hair sample. The method can reduce the consumption of the sample and the acid liquor, reduce the sample transfer process and accelerate the large sample treatment process while ensuring the experiment requirements, and has the advantages of simplified process, stable process control, simple operation and wide applicability.

Description

technical field [0001] The invention relates to the technical field of essential oil preparation, in particular to a hair sample pretreatment method using a dry-wet mixing method. Background technique [0002] The health status of the human body and the research on trace elements in the human body are getting more and more attention, and the trace elements in the hair are relatively stable. Determining the content of trace elements in the hair is an effective way to evaluate the status of the elements in the human body. The determination methods of trace elements include electrochemical methods , spectrometry, mass spectrometry, etc., including polarographic analysis, anodic stripping voltammetry, spectrophotometry, molecular luminescence analysis, atomic absorption spectrometry, atomic emission spectrometry, atomic fluorescence spectrometry, neutron activation, inductance Coupled plasma mass spectrometry, etc., all of these methods require pretreatment of the sample. [00...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/34G01N1/44
CPCG01N1/28G01N1/34G01N1/44
Inventor 胡习超胡习文巫新春
Owner 安徽新生医学科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap