Application of member 7 of solute carrier family 7 in the preparation of drugs for preventing and treating enterovirus 71 infection

An enterovirus, vector technology, applied in the field of biomedicine

Active Publication Date: 2021-07-02
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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Problems solved by technology

[0007] However, there is no report on the role of ABCC3 and SLC7A7 molecules in virus-infected host cells. In-depth research on these two molecules will not only improve the understanding of EV71 infection and pathogenic mechanisms, but also contribute to the prevention and treatment of EV71 infection. Provide new ideas and targets

Method used

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  • Application of member 7 of solute carrier family 7 in the preparation of drugs for preventing and treating enterovirus 71 infection
  • Application of member 7 of solute carrier family 7 in the preparation of drugs for preventing and treating enterovirus 71 infection
  • Application of member 7 of solute carrier family 7 in the preparation of drugs for preventing and treating enterovirus 71 infection

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Embodiment 1

[0062] 1 Design and synthesis of specific siRNA sequences for each transport-related membrane protein.

[0063] 1.1 For each target gene, search NCBI GeneBank to obtain the full sequence and mRNA sequence, use existing network resources and commonly used software to conduct biological analysis on AXIIR, and select the coding region as the target sequence for siRNA design. Refer to the siRNA design principle, and compare it with the human genome sequence through the blast function of the GeneBank database to ensure that there is no homology; exclude potential siRNAs that have 8 consecutive bases at the 5' end of the aitisense chain paired with other genes; exclude any consecutive 14 bases base-pairing potential siRNAs with other genes. The design software was used for pre-evaluation and determination, and three targets with the best kinetic parameters were selected to enter the subsequent experimental process. A total of three interference sequences were synthesized for each ge...

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Abstract

The invention relates to the technical field of biomedicine, in particular to a new target for resisting enterovirus 71 infection and its application. In the present invention, human colon cancer cells (Caco-2) are used as target cells, and RNA interference technology is used to down-regulate the expression of target cell transport-related membrane proteins to find host factors that can effectively inhibit EV71 from infecting human colon cancer cells, so as to achieve Road) for the purpose of blocking EV71 infection. The present invention finds through experiments that solute carrier family 7 member 7 (SLC7A7) plays an important role in EV71 infection of Caco‑2 cells, and downregulating the expression of SLC7A7 can significantly inhibit EV71 infection. The invention provides the application of SLC7A7 in the preparation of drugs for the prevention or treatment of enterovirus 71 infection, and provides clinical prevention and treatment for diseases such as hand, foot and mouth disease, meningitis, brainstem encephalitis or polio caused by EV71 infection. new targets and treatment options.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a new target and application of anti-enterovirus 71 infection. Background technique [0002] Enterovirus 71 (EV71) belongs to the Picornaviridae Enterovirus genus Human Enterovirus A, and is one of the main pathogens that cause Hand-foot-mouth disease (HFMD). Currently, hand, foot and mouth disease is breaking out and spreading in many parts of the world, especially in the Asia-Pacific region. In my country, since the outbreak of hand, foot and mouth disease in several major provinces and cities in 2008, the number of people infected and the death rate of the disease have remained high, with more than 1 million cases and nearly 1,000 deaths reported every year. The main patients with HFMD are infants and young children under 5 years old. The clinical manifestations are fever, herpes and other symptoms on the hands, feet, buttocks and oral mucosa; a small number of children c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61P31/14C12Q1/6883
CPCA61K45/00A61P31/14C12Q1/6883C12Q2600/158
Inventor 朱勇喆周勇涛施赟杰刘延刚戚中田赵平任浩
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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