Novel assay for the diagnosis of nematode infections

A useful technique for roundworm infection, applied in the field of somatic cell lysate of Toxocara canis larvae, which can solve the problem of not being able to provide high specificity and high sensitivity at the same time

Pending Publication Date: 2021-01-05
EUROIMMUN MEDIZINISCHE LABORDIAGNOSTIKA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, current immunological Toxocara assays do not provide both high specificity and high sensitivity

Method used

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  • Novel assay for the diagnosis of nematode infections
  • Novel assay for the diagnosis of nematode infections
  • Novel assay for the diagnosis of nematode infections

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0081] The following experiments were performed to evaluate different Toxocara antigens in an indirect ELISA for the detection of specific anti-Tx IgG antibodies in human serum or plasma. The following antigens were analyzed a) for sensitivity, b) specificity for Toxocara negative patient samples and c) specificity for samples positive for other parasitic diseases.

[0082] Description of Toxocara species antigens tested

[0083] The following antigens were analyzed: 1) recombinant mTES-30 from Toxocara species, 2) somatic antigen from T. canis larvae, 3) excretory secreted (E / S) antigen from T. canis larvae and 4) T. canis larvae. Somatic antigen binding to recombinant mTES-30.

[0084] sample:

[0085] Group 1 (sensitivity group)

[0086] The sensitivity panel contained samples from 16 patients with confirmed Toxocara infection. Samples were serologically determined by ELISA and / or blot techniques ( figure 1 ).

[0087] Group 2 (specificity group)

[0088] This specif...

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Abstract

The present invention relates to a diagnostically useful carrier comprising (a) a peptide comprising the amino acid sequence set forth in SEQ ID NO:1 or a variant thereof, and (b) a somatic lysate ofToxocara canis larvae. Further the present invention relates to a kit, use, methods, compositions comprising in the diagnostically useful carrier of the invention. A method for diagnosing a Toxocara infection, comprising detecting in a sample of a patient the presence of an antibody to SEQ ID NO:1 and / or an antibody to a somatic lysate of Toxocara canis larvae, is also disclosed.

Description

technical field [0001] The present invention relates to a diagnostically useful vector comprising (a) a peptide comprising the amino acid sequence shown in SEQ ID NO: 1 or a variant thereof, and (b) a somatic cell lysate of Toxocara canis larvae. Furthermore, the present invention relates to kits, uses, methods, compositions comprising the diagnostically useful vector of the present invention. Background technique [0002] Toxocarasis is a helminth infection in humans caused by roundworms, Toxocara canis or Toxocara cati. This infection occurs upon ingestion of embryonated eggs containing fully developed infective larvae of Toxocara species. Larvae in the human gut penetrate the gut wall and migrate through blood vessels to the liver, muscles and lungs, and even into the eyes and brain. [0003] Toxocarasis is commonly referred to as visceral larva migrans (VLM). Other names or associated symptoms may be ocular larva migration (OLM), Weingarten's disease, Frimodt- Syndr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/543
CPCG01N33/6854G01N33/543G01N2333/4353G01N33/5085G01N2469/20C07K14/4354C07K16/18G01N33/6893G01N2800/26
Inventor B·蒙格K·斯特恩哈格J·谢弗C·普罗布斯特A·德尔伯格M·尤恩格C·麦司林
Owner EUROIMMUN MEDIZINISCHE LABORDIAGNOSTIKA
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