Method for constructing HPLC fingerprint spectrum of hair growth tablet

A technology of fingerprint spectrum and construction method, applied in the field of traditional Chinese medicine analysis, can solve the problems of complex construction method and cannot represent the quality level, etc., and achieve the effects of high extraction efficiency, perfect quality evaluation system and good peak shape.

Active Publication Date: 2021-01-12
GUANGXI NANNING BAIHUI PHARMA GRP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the prior art, in the construction method of the HPLC fingerprint of the traditional Chinese medicine preparation, the determination of the content of a certain significant component is often used, and the efficacy of the traditional Chinese medicine preparation is often the result of the joint action of multiple active components. Therefore, the content of a certain component Qualified cannot represent the overall quality level and has great limitations
The Chinese invention patent with the notification number CN103926351B discloses a quality detection method for Shengxuebao preparations and a method for constructing standard fingerprints. Shengxuebao was tested for quality, with paeoniflorin as the reference substance, and 7 characteristic peaks were respectively identified to achieve the purpose of detecting the contents of 7 kinds of medicines in the prescription. The exclusive characteristic peak of Astragalus membranaceus, the other three medicines correspond to the common peak, and the method of constructing the map is complicated, which is not suitable for traditional Chinese medicine preparations with multiple prescriptions

Method used

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  • Method for constructing HPLC fingerprint spectrum of hair growth tablet
  • Method for constructing HPLC fingerprint spectrum of hair growth tablet
  • Method for constructing HPLC fingerprint spectrum of hair growth tablet

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Construction of HPLC Fingerprint of Germinal Tablets

[0042] 1. Preparation of the test solution: take the hair growth tablets of this product, remove the coating, grind finely to obtain the test powder, take 0.5g of the test powder, accurately weigh it, place it in a stoppered conical flask, and accurately weigh it. Add 10mL of solvent, weigh it, ultrasonically treat it (power 300W, frequency 40kHz) for 30 minutes, let it cool, weigh it again, make up for the lost weight with solvent, shake well, pass through a 0.45μm filter membrane, and take the subsequent filtrate, namely have to;

[0043] 2. Preparation of reference substance solution: Weigh an appropriate amount of stilbene glucoside, daidzin, privetin, and paeoniflorin reference substances respectively, place them in the same volumetric flask, add solvent to make a mixture containing 50 μg of each of the above reference substances per mL Reference substance solution;

[0044] 3. The preparation of medicinal ma...

Embodiment 2

[0095] Construction of HPLC Fingerprint of Germinal Tablets

[0096] 1. Preparation of the test solution: take the hair growth tablets of this product, remove the coating, grind finely to obtain the test powder, take 0.5g of the test powder, accurately weigh it, place it in a stoppered conical flask, and accurately weigh it. Add 9mL of solvent, weigh it, sonicate (power 250W, frequency 35kHz) for 29 minutes, let it cool, weigh again, use solvent to make up for the lost weight, shake well, pass through a 0.45μm filter membrane, and take the subsequent filtrate, namely have to;

[0097] 2. Preparation of reference substance solution: Weigh an appropriate amount of stilbene glucoside, daidzin, privetin, and paeoniflorin reference substances respectively, place them in the same volumetric flask, and add solvent to make a mixture containing 52 μg of each of the above reference substances per mL. Reference substance solution;

[0098] 3. The preparation of medicinal material test ...

Embodiment 3

[0104] Construction of HPLC Fingerprint of Germinal Tablets

[0105] 1. Preparation of the test solution: take the hair growth tablets of this product, remove the coating, grind finely to obtain the test powder, take 0.5g of the test powder, accurately weigh it, place it in a stoppered conical flask, and accurately weigh it. Add 9.5mL of solvent, weigh it, ultrasonically treat it (power 270W, frequency 36kHz) for 28 minutes, let it cool, weigh it again, make up for the lost weight with solvent, shake well, pass through a 0.45μm filter membrane, and take the subsequent filtrate. instant;

[0106] 2. Preparation of reference substance solution: Weigh an appropriate amount of stilbene glucoside, daidzin, privetin, and paeoniflorin reference substances respectively, place them in the same volumetric flask, and add solvent to make a mixture containing 55 μg of each of the above reference substances per mL. Reference substance solution.

[0107] 3. The preparation of medicinal mat...

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Abstract

The invention discloses a method for constructing an HPLC (high performance liquid chromatography) fingerprint spectrum of a hair growth tablet, belongs to the technical field of traditional Chinese medicine analysis, and the method takes a hair growth tablet traditional Chinese medicine tablet prepared by taking traditional Chinese medicines including polygonum multiflorum, glossy privet fruit, eclipta, Rehmanniae Radix, Chinese yam, poria cocos, moutan bark, rhizoma alismatis, mulberry, radix ophiopogonis, black date and black soya bean as detection targets, obtains the high performance liquid chromatography fingerprint of the hair growth tablet by adopting a high performance liquid chromatography method. According to the fingerprint spectrum constructed by the method, 10 common characteristic peaks exist, 10 chemical components are determined and identified by utilizing a DAD (Digital Analog-Digital) detector and a UFLC-TRIPLETOF-DAD-MS/MS technical means. The quality control methodprovided by the invention can comprehensively and systematically detect the medicinal materials in the prescription, performs quality control on the whole process of the preparation production process and evaluates the quality of the final product, and has a practical application value.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine analysis, and in particular relates to a method for constructing an HPLC fingerprint of a hair growth tablet made from raw materials of traditional Chinese medicine. Background technique [0002] The hair growth tablet used in the present invention is a traditional Chinese medicine tablet made from Chinese medicine Polygonum multiflorum, Ligustrum lucidum, Eclipta chinensis, Rehmannia glutinosa, Chinese yam, Poria cocos, Moutan cortex, Alisma, mulberry, Ophiopogon japonicus, black dates, and black beans. Liver and kidney, nourishing qi and blood, hair growth and black hair function. It is used for premature graying and loss of hair caused by deficiency of liver and kidney, deficiency of Qi and blood; alopecia areata, total alopecia, seborrheic alopecia, and has broad clinical application prospects. The invention provides a method for constructing an HPLC fingerprint of a hair...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/72G01N30/8634G01N30/8686
Inventor 姚宏亮刘颖彭维周雪芸王永刚李硕俊苏薇薇黄丽巧
Owner GUANGXI NANNING BAIHUI PHARMA GRP
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