Tissue culture and rapid propagation seedling raising method for large-purple-leaf variegata-provided haworthia cooperi plant

A technology of rapid propagation of seedlings, tissue culture and rapid propagation, which is applied in botany equipment and methods, planting substrates, gardening methods, etc., can solve the problems of breeding germs and pests, and achieve the effects of increased air permeability, healthy color, and soil grabbing Effect

Inactive Publication Date: 2021-01-29
重庆绿莱生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Organic matter also breeds germs and pests

Method used

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  • Tissue culture and rapid propagation seedling raising method for large-purple-leaf variegata-provided haworthia cooperi plant
  • Tissue culture and rapid propagation seedling raising method for large-purple-leaf variegata-provided haworthia cooperi plant
  • Tissue culture and rapid propagation seedling raising method for large-purple-leaf variegata-provided haworthia cooperi plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The tissue culture rapid propagation seedling raising method of embodiment 1 Daziyulujin plant

[0034] S1: Obtaining the aseptic mutant plants of Daziyulu:

[0035]Through the large-scale tissue culture and rapid propagation of ordinary Daziyulu, from a large number of sterile seedlings produced, the leaves are obviously changed from green to yellow or red, and the discoloration is uniform, pulled from the tip of the leaf to the The base of the leaf, the color penetrates the leaf surface and the back of the leaf, and the stable mutant plant - Daziyulujin, is used as the female parent to establish a sterile and rapid propagation system for Daziyulujin plants;

[0036] S2: Establishment of the rapid propagation system of Daziyulujin plants:

[0037] S21: Cutting of Daziyulujin: On the ultra-clean workbench, carefully peel off the outermost 3 to 5 leaves of Daziyulujin along the base with a sterile scalpel blade and tweezers. When peeling off the leaf base must have gro...

Embodiment 2

[0044] The tissue culture rapid propagation seedling raising method of embodiment 2 Daziyulujin plants

[0045] The difference from the above example is that the tissue culture of Daziyulujin can also use the remaining four leaves in the center of the plant after peeling off the leaves, and leave four or more leaves without peeling off, and insert the improved MS strong seedling medium together with the base of the plant After 28 days, the center of the plant with the base in the strong seedling medium grows new leaves, which become sub-adult seedlings, and the sub-adult seedlings are transferred to the improved MS rooting medium for cultivation; after 28 days, the sub-adult seedlings are It can be cultivated into tissue culture seedlings of finished Daziyulujin.

[0046] Repeat the above steps with the tissue cultured seedlings obtained in the above-mentioned embodiment 1 or embodiment 2, and perform cyclic operation to obtain cultivated seedlings through cultivation and mana...

Embodiment 3

[0047] Example 3 Effects of Different Cutting Methods on the Proliferation Mode of Daziyulujin

[0048] T1 adopts the method of embodiment 1 to cut,

[0049] T2 adopts no cutting, the whole plant is inserted, and the ramets are multiplied.

[0050] T3 cut off the leaves and put them into the culture medium,

[0051] Table 1 below shows the effect of different cutting methods on the multiplication mode of Daziyulujin.

[0052] Table 1 Comparison of the effects of different cutting methods on the multiplication mode of Daziyulujin

[0053]

[0054] As can be seen from the above table 1: under the same culture conditions, the proliferation rate of T1 (the present invention) is 5 to 7 times that of T2, reaching the highest 28 times, which greatly improves the proliferation efficiency; and the callus is only 5% , the leaves with callus can be discarded to avoid the variation caused by callus re-differentiation into seedlings, the results are shown in figure 1 (A); T2 has no ...

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Abstract

The invention discloses a tissue culture and rapid propagation seedling raising method for a large-purple-leaf variegata-provided haworthia cooperi plant. The method comprises the following steps of selecting large-purple-leaf haworthia cooperi sterile variant plants to establish a rapid propagation system through an artificial screening method; and by improving a proliferation culture medium, a strong seedling culture medium and a rooting culture medium and optimizing a culture medium formula and maintenance management, obtaining the healthier, more beautiful and more stable plants. The proliferation efficiency of the large-purple-leaf variegata-provided haworthia cooperi propagated by the method is high, the germination rate and rooting rate of the variant plants are three times those ofa conventional culture medium, finished culture seedlings are strong and bright in color, the propagation coefficient and propagation speed are effectively increased by improving the culture medium,the transplanting survival rate of tissue culture seedlings is effectively increased by 23% by improving the culture medium, in addition, the transplanting hardening survival rate of the tissue culture seedlings can be improved to 80 to 93%, the seedling plants cultured by the method are consistent in shape and easy to operate and facilitate industrial production, and rare variant plants can be moderately produced in batch in a short time and put into the market.

Description

technical field [0001] The invention belongs to the field of plant group cultivation of seedlings, and in particular relates to a method for rapid propagation of seedlings by tissue culture of succulent succulents of the genus Twelve Volumes of Liliaceae. Background technique [0002] In recent years, succulents have attracted more and more attention in the market, with various varieties, grades and prices. Twelve volumes of Liliaceae belong to a category of succulents, which have high ornamental and collection value. Many species are rare and expensive, and the reproduction rate is very low. However, the market demand is large, resulting in high prices for rare varieties, especially imported varieties, especially mutant strains. At present, the number of succulent plant variants on the market is extremely rare, and the price is extremely expensive. The regularized variants cost tens of thousands, and some even hundreds of thousands of plants. Therefore, a large number of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G24/42A01G24/28A01G24/22A01G24/20A01G24/12A01G24/30
CPCA01H4/001A01H4/005A01H4/008A01G24/12A01G24/20A01G24/22A01G24/28A01G24/30A01G24/42
Inventor 郭芳琴黄丽丹
Owner 重庆绿莱生物科技有限公司
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