Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of sea cucumber powder in preparation of medicine for promoting skin wound healing

A technology for skin wounds and sea cucumber powder, which can be used in drug combinations, skin diseases, medical raw materials derived from echinoderms, etc., can solve problems such as lack of universality, and achieve the effect of regulating inflammatory response and promoting regeneration

Pending Publication Date: 2021-01-29
JIANGNAN UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One article analyzed the effect of sea cucumber polysaccharides on wound healing in mice, but only the apparent indicators such as visual observation and wound microscope magnification comparison results were not involved in the internal indicators
Another article analyzed the effect of sea cucumber collagen oligopeptides on the postoperative wound healing of diabetic model mice. The object is a diabetic model, which is not universal

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of sea cucumber powder in preparation of medicine for promoting skin wound healing
  • Application of sea cucumber powder in preparation of medicine for promoting skin wound healing
  • Application of sea cucumber powder in preparation of medicine for promoting skin wound healing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] Embodiment 1: the preparation of the enzymolysis solution of different kinds of enzymes

[0110] (1) Cut open the fresh sea cucumber from the abdomen, remove internal organs and gonads, soak in clean water for 3-5 minutes, and then wash the inner and outer surfaces of the sea cucumber body wall with running water;

[0111] (2) Drain the washed sea cucumber body wall, cut it into small pieces (1~2)cm×(1~2)cm and put it in a multifunctional blender, which does not exceed 80% of the volume of the container. Under the condition of water, stir at low speed for homogenization for 1 min;

[0112] (3) Take 160g of homogenate and add it to the fermentation bottle, then add 640g of water and 112000U protease, the enzymolysis temperature is 50-55°C, the pH is natural pH (6.5-7.5), and the enzymolysis time is 5h;

[0113] (4) Heat in a boiling water bath at 95-100°C for 15 minutes to inactivate the enzyme;

[0114] (5) Centrifuge at 10000rpm / min and 4°C for 20min, collect the sup...

Embodiment 2

[0119] Embodiment 2: the preparation of the enzymolysis solution of compound enzyme of different ratio

[0120] (1) Cut open the fresh sea cucumber from the abdomen, remove internal organs and gonads, soak in clean water for 3-5 minutes, and then wash the inner and outer surfaces of the sea cucumber body wall with running water;

[0121] (2) Drain the washed sea cucumber body wall, cut it into small pieces (1~2)cm×(1~2)cm and put it in a multifunctional blender, which does not exceed 80% of the volume of the container. Under the condition of water, stir at low speed for homogenization for 1 min;

[0122] (3) Take 160g of homogenate and add it to a fermentation bottle, then add 640g of water and a compound protease with a total enzyme amount of 112000U, the enzymolysis temperature is 50-55°C, the pH is natural pH (6.5-7.5), and the enzymolysis time is 5h .

[0123] (4) Heat in a boiling water bath at 95-100°C for 15 minutes to inactivate the enzyme;

[0124] (5) Centrifuge a...

Embodiment 3

[0129] Embodiment 3: the preparation of different molecular weight sea cucumber powder

[0130] (1) Cut open the fresh sea cucumber from the abdomen, remove internal organs and gonads, soak in clean water for 3-5 minutes, and then wash the inner and outer surfaces of the sea cucumber body wall with running water;

[0131] (2) Drain the washed sea cucumber body wall, cut it into small pieces (1~2)cm×(1~2)cm and put it in a multifunctional blender, which does not exceed 80% of the volume of the container. Under the condition of pure water, stir at low speed for homogenization for 1 min;

[0132] (3) Take 160g of homogenate and add it to the fermentation bottle, then add 640g of pure water and 112000U compound enzyme (67200U special enzyme for aquatic products + 44800U flavor protease), the enzymolysis temperature is 50~55℃, and the pH is natural pH (6.5~7.5) ;

[0133] (4) Heat in a boiling water bath at 95-100°C for 15 minutes to inactivate the enzyme;

[0134] (5) Centrifug...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of sea cucumber powder in preparation of a medicine for promoting skin wound healing, and belongs to the field of aquatic product utilization. The preparation method of the sea cucumber powder in the application comprises the following steps: (1) removing internal organs and gonads of sea cucumbers, and carrying out soaking and washing; (2) carrying out homogenizing; (3) adding water and a compound enzyme into the homogenate for enzymolysis, wherein the compounding ratio of the compound enzyme is that the ratio of the special enzyme for aquatic products to flavourzyme is (2-8): (2-8) (U); (4) carrying out enzyme deactivation and cooling; (5) carrying out centrifuging; (6) carrying out rotary evaporation and concentration; (7) carrying out storing and freezing; and (8) carrying out freeze-drying to obtain the sea cucumber powder. According to the invention, inflammatory response and oxidative stress degree of rats with skin wounds can be significantlyadjusted, regeneration of skin collagen fibers at the wound surfaces of the rats is promoted, secretion of VEGF, EGF and bFGF at the wound surfaces is promoted, and the application prospect in the field of wound surface healing is achieved.

Description

technical field [0001] The invention relates to the application of sea cucumber powder in the preparation of medicines for promoting skin wound healing, and belongs to the field of aquatic product utilization. Background technique [0002] Cutaneous trauma refers to the breakdown of the epithelial integrity of the skin and may be accompanied by disruption of the structure and function of the underlying normal tissue. The type and depth of trauma, the health status of the injured subject, the nutritional status and its tissue repair ability will all affect the speed of wound healing, and slow or improper wound healing will affect the quality of life of the injured subject, and cause bacterial infection and other diseases. risk of complications. Wound healing is a complex dynamic process, which will go through four stages of hemostasis, inflammation, proliferation and remodeling to complete the repair, which is inseparable from the interaction of many inflammatory factors and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/616A61P17/02A61P29/00
CPCA61K35/616A61P17/02A61P29/00
Inventor 周鹏胡锦华程敏君杨亭亭金跃杰张超越梁咏雪
Owner JIANGNAN UNIV