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Urechis unicinctus polypeptide with angiotensin converting enzyme inhibitory activity and application thereof

A technology of angiotensin and monocyclic stinger, which is applied in the field of biomedicine, can solve the problems of no record and report of ACE inhibitory peptides, and achieve the effect of increasing eNOS activity and increasing the content of bradykinin

Inactive Publication Date: 2021-03-09
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, there is no record and report about the ACE inhibitory peptide derived from the monocyclic thorn in the prior art

Method used

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  • Urechis unicinctus polypeptide with angiotensin converting enzyme inhibitory activity and application thereof
  • Urechis unicinctus polypeptide with angiotensin converting enzyme inhibitory activity and application thereof
  • Urechis unicinctus polypeptide with angiotensin converting enzyme inhibitory activity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Effect of the monocyclic echinococcoid polypeptide of the present invention on blood pressure regulatory factors in vascular endothelial cells

[0031] 1. Experimental materials and instruments

[0032] 1.1 Experimental materials

[0033] F2: Ile-Val-Lys-His-Phe

[0034] F7: Ile-Val-Met-Arg-Ile

[0035] Human umbilical vein endothelial cells (HUVEC) were provided by the Cell Bank of Shanghai Institute of Biological Sciences, Chinese Academy of Sciences.

[0036] 1.2 Main reagents see Table 1

[0037] Table 1 Main Reagents

[0038]

[0039]

[0040] 1.3 Experimental Instrument Participation Table 2

[0041] Table 2 Experimental Instruments

[0042]

[0043] 2 Experimental methods

[0044] 2.1 Peptide inhibition mode

[0045] According to the methods reported in previous studies (see [Forghani B, Zarei M, Ebrahimpour A, et al. Purification and characterization of angiotensin converting enzyme-inhibitory peptides derived from Stichopus horrens: S...

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Abstract

The invention provides urechis unicinctus polypeptide with angiotensin converting enzyme inhibitory activity and application thereof. The urechis unicinctus polypeptide comprises a first polypeptide and / or a second polypeptide, wherein the amino acid sequence of the first polypeptide is as shown in SEQ ID NO. 1; and the amino acid sequence of the second polypeptide is as shown in SEQ ID NO. 2. Theurechis unicinctus polypeptide F2 and the urechis unicinctus polypeptide F7 have no inhibiting effect on proliferation of HUVEC cells, can antagonize the effect of noradrenaline (NE) on inhibiting NOrelease in the cells, and can inhibit the effect of promoting cell release ET-1 caused by the NE, so that the purpose of reducing blood pressure is achieved. According to the urechis unicinctus polypeptide, the activity of ACE is competitively inhibited, the content of bradykinin is increased, and the phosphorylation effect of endothelial nitric oxide synthase (eNOS) is influenced, so that the eNOS activity is improved, more NO is released, ET-1 is inhibited, and the effect of reducing blood pressure is achieved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a monocyclic echinacea polypeptide with angiotensin-converting enzyme inhibitory activity and application thereof. Background technique [0002] Hypertension is a condition in which the pressure in blood vessels continues to rise. This disease greatly increases the risk of heart, brain, kidney and other organs, and also greatly increases the risk of other diseases. [0003] Angiotensin-converting enzyme plays an important role in regulating blood pressure by converting angiotensin I to the octapeptide angiotensin II, which has a strong vasoconstrictive effect, and degrades bradykinin through vasodilation. ACE inhibitory peptides are also called angiotensin-converting enzyme inhibitory peptides, which can competitively bind to the two active functional domains of ACE, inhibit ACE activity, and inhibit the conversion of angiotensin I to angiotensin II. At present, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61K38/08A61P9/12
CPCC07K7/06A61P9/12A61K38/00
Inventor 孙坤来初雪梅陈荫李佩佩王斌赵玉勤王玉梅张译文
Owner ZHEJIANG OCEAN UNIV
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